LOCUS       .                       5794 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     rep_origin      487..709
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             757..1704
                     /label="Rep101(Ts)"
                     /note="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
     promoter        complement(3191..3295)
                     /label="AmpR promoter"
     CDS             3646..4458
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     primer_bind     5406..5422
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    5423..5479
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5492..5508)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5516..5532)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5540..5570)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5585..5606)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 gatccccgac agtaagacgg gtaagcctgt tgatgatacc gctgccttac tgggtgcatt
       61 agccagtctg aatgacctgt cacgggataa tccgaagtgg tcagactgga aaatcagagg
      121 gcaggaactg ctgaacagca aaaagtcaga tagcaccaca tagcagaccc gccataaaac
      181 gccctgagaa gcccgtgacg ggcttttctt gtattatggg tagtttcctt gcatgaatcc
      241 ataaaaggcg cctgtagtgc catttacccc cattcactgc cagagccgtg agcgcagcga
      301 actgaatgtc acgaaaaaga cagcgactca ggtgcctgat ggtcggagac aaaaggaata
      361 ttcagcgatt tgcccgagct tgcgagggtg ctacttaagc ctttagggtt ttaaggtctg
      421 ttttgtagag gagcaaacag cgtttgcgac atccttttgt aatactgcgg aactgactaa
      481 agtagtgagt tatacacagg gctgggatct attcttttta tcttttttta ttctttcttt
      541 attctataaa ttataaccac ttgaatataa acaaaaaaaa cacacaaagg tctagcggaa
      601 tttacagagg gtctagcaga atttacaagt tttccagcaa aggtctagca gaatttacag
      661 atacccacaa ctcaaaggaa aaggactagt aattatcatt gactagccca tctcaattgg
      721 tatagtgatt aaaatcacct agaccaattg agatgtatgt ctgaattagt tgttttcaaa
      781 gcaaatgaac tagcgattag tcgctatgac ttaacggagc atgaaaccaa gctaatttta
      841 tgctgtgtgg cactactcaa ccccacgatt gaaaacccta caaggaaaga acggacggta
      901 tcgttcactt ataaccaata cgttcagatg atgaacatca gtagggaaaa tgcttatggt
      961 gtattagcta aagcaaccag agagctgatg acgagaactg tggaaatcag gaatcctttg
     1021 gttaaaggct ttgagatttt ccagtggaca aactatgcca agttctcaag cgaaaaatta
     1081 gaattagttt ttagtgaaga gatattgcct tatcttttcc agttaaaaaa attcataaaa
     1141 tataatctgg aacatgttaa gtcttttgaa aacaaatact ctatgaggat ttatgagtgg
     1201 ttattaaaag aactaacaca aaagaaaact cacaaggcaa atatagagat tagccttgat
     1261 gaatttaagt tcatgttaat gcttgaaaat aactaccatg agtttaaaag gcttaaccaa
     1321 tgggttttga aaccaataag taaagattta aacacttaca gcaatatgaa attggtggtt
     1381 gataagcgag gccgcccgac tgatacgttg attttccaag ttgaactaga tagacaaatg
     1441 gatctcgtaa ccgaacttga gaacaaccag ataaaaatga atggtgacaa aataccaaca
     1501 accattacat cagattccta cctacataac ggactaagaa aaacactaca cgatgcttta
     1561 actgcaaaaa ttcagctcac cagttttgag gcaaaatttt tgagtgacat gcaaagtaag
     1621 tatgatctca atggttcgtt ctcatggctc acgcaaaaac aacgaaccac actagagaac
     1681 atactggcta aatacggaag gatctgaggt tcttatggct cttgtatcta tcagtgaagc
     1741 atcaagacta acaaacaaaa gtagaacaac tgttcaccgt tacatatcaa agggaaaact
     1801 gtccatatgc acagatgaaa acggtgtaaa aaagatagat acatcagagc ttttacgagt
     1861 ttttggtgca tttaaagctg ttcaccatga acagatcgac aatgtaacag atgaacagca
     1921 tgtaacacct aatagaacag gtgaaaccag taaaacaaag caactagaac atgaaattga
     1981 acacctgaga caacttgtta cagctcaaca gtcacacata gacagcctga aacaggcgat
     2041 gctgcttatc gaatcaaagc tgccgacaac acgggagcca gtgacgcctc ccgtggggaa
     2101 aaaatcatgg caattctgga agaaatagcg ctttcagccg gcaaaccggc tgaagccgga
     2161 tctgcgattc tgataacaaa ctagcaacac cagaacagcc cgtttgcggg cagcaaaacc
     2221 cgtacttttg gacgttccgg cggttttttg tggcgagtgg tgttcgggcg gtgcgcgcaa
     2281 gatccattat gttaaacggg cgagtttaca tctcaaaacc gcccgcttaa caccatcaga
     2341 aatcctcagc gcgattttaa gcaccaaccc ccccccgtaa cacccaaatc catactgaaa
     2401 gtggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcctcc
     2461 cgacaacaca gaccattccg tggcaaagca aaagttcaga atcaccaact ggtccaccta
     2521 caacaaagct ctcatcaacc gtggctccct cactttctgg ctggatgatg aggcgattca
     2581 ggcctggtat gagtcggcaa caccttcatc acgaggaagg ccccagcgct attctgatct
     2641 cgccatcacc accgttctgg tgattaaacg cgtattccgg ctgaccctgc gggctgcgca
     2701 gggttttatt gattccattt ttgccctgat gaacgttccg ttgcgctgcc cggattacac
     2761 cagtgtcagt aagcgggcaa agtcggttaa tgtcagtttc aaaacgtcca cccggggtga
     2821 aatcgcacac ctggtgattg attccaccgg gctgaaggtc tttggtgaag gcgaatggaa
     2881 agtcagaaag cacggcaaag agcgccgtcg tatctggcga aagttgcatc ttgctgtggg
     2941 gatcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3001 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3061 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3121 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3181 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3241 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3301 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     3361 aaaataggcg tatcacgagg ccctttcgtc ttcgaataaa tacctgtgac ggaagatcac
     3421 ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt
     3481 tggcgaaaat gagacgtgat acttctattc tactctgact gcaaaccctg cagggaaagc
     3541 cacgttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     3601 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     3661 cgggaaacgt cttgctcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     3721 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg
     3781 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     3841 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     3901 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca
     3961 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     4021 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     4081 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     4141 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataagctt
     4201 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     4261 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     4321 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     4381 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     4441 atgctcgatg agtttttcta atttttttaa ggcagttatt ggtgccctta aacgcctggt
     4501 tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgaa agcaaattcg
     4561 acccggtcgt cggttcaggg cagggtcgtt aaatagccgc ttatgtctat tgctggttta
     4621 ccggtttatt gactaccgga agcagtgtga ccgtgtgctt ctcaaatgcc tgaggccagt
     4681 ttgctcaggc tctccccgtg gaggtaataa ttgacgatat gatcatttat tctgcctccc
     4741 agagcctgat aaaaacggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga
     4801 ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc
     4861 gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac
     4921 gatcagcggt ccaatgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg
     4981 tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat
     5041 gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc
     5101 cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc
     5161 gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa
     5221 taccgcaagc gacaggccat gtttgacagc ttatcatcga taagctcatt cgccattcag
     5281 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     5341 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     5401 acgttgtaaa acgacggcca gtgaattcga gctcggtacc cggggatcct ctagagtcga
     5461 cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5521 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5581 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5641 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5701 gtattggcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     5761 ttcctaatgc aggagtcgca taagggagag cgtc
//
