LOCUS       V014368                 7924 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014368
VERSION     V014368
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             66..800
                     /note="Isopentenyl phosphate kinase from Thermoplasma
                     acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC
                     15155 / AMRC-C165). Accession#: Q9HLX1"
                     /label="Isopentenyl phosphate kinase"
     CDS             801..818
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      865..913
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1151..1606
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1728..1832
                     /label="AmpR promoter"
     CDS             1833..2690
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2864..3455
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    3743..3764
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        complement(4225..4327)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             4866..5867
                     /label="repB"
                     /note="RepB replication protein"
ORIGIN
        1 catatgagtt atgcagtttg tagaatgcaa aaagtgaaat cagggggatc atggatcctt
       61 tcacaatgat gatccttaaa atcggcggct ctgttatcac agataaatct gcttaccgta
      121 cagctcgtac atacgctatc cgttctatcg ttaaagttct ttctggcatc gaagatcttg
      181 tttgcgttgt tcatggcggc ggctctttcg gccatatcaa agctatggaa ttcggccttc
      241 ctggccctaa aaaccctcgt tcttctatcg gctactctat cgttcatcgt gatatggaaa
      301 accttgatct tatggttatc gatgctatga tcgaaatggg catgcgtcct atctctgttc
      361 ctatctctgc tcttcgttat gacggcaggt tcgattacac accacttatc cgttacatcg
      421 atgctggctt cgttcctgtt tcttacggcg atgtttacat caaagatgaa cattcttacg
      481 gcatctactc tggcgatgat atcatggctg atatggctga acttcttaaa cctgatgttg
      541 ctgttttcct tacagatgtt gatggcatct actctaaaga tcctaaacgt aaccctgatg
      601 ctgttcttct tcgtgatatc gatacaaaca tcacattcga tcgtgttcaa aacgatgtta
      661 caggcggcat cggcaaaaaa ttcgaatcta tggttaaaat gaaatcttct gttaagaatg
      721 gcgtgtacct gatcaacggc aaccatcctg aacgtatcgg cgatatcggc aaagaatctt
      781 tcatcggcac agttatccgt catcatcatc atcatcacta acgcgttcta gaggtcgaaa
      841 ttcacctcga aagcaagctg ataaaccgat acaattaaag gctccttttg gagccttttt
      901 ttttggagat tttcaacgtg aaaaaattat tattcgcaat tccaagctaa ttcacctcga
      961 aagcaagctg ataaaccgat acaattaaag gctccttttg gagccttttt ttttggagat
     1021 tttcaacgtg aaaaaattat tattcgcaat tccaagctct gcctcgcgcg tttcggtgat
     1081 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     1141 gatgcagatc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     1201 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     1261 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     1321 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     1381 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     1441 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     1501 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     1561 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttgatc tgcgctcggt
     1621 cgttcggctg cggcgagcgg tatcagctca ctcaaagcgg taatacggtt atcccagaat
     1681 ccggggataa cgcaggaaag acaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1741 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1801 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     1861 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     1921 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     1981 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2041 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     2101 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2161 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2221 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     2281 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     2341 ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca
     2401 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     2461 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     2521 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     2581 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2641 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2701 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2761 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2821 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2881 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2941 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3001 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3061 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3121 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3181 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaagtactga
     3241 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3301 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3361 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3421 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3481 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3541 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3601 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3661 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     3721 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     3781 tacactttat gcttccggca tattctcaat aaacccttta gggaaatagg ccaggttttc
     3841 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     3901 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     3961 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc
     4021 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     4081 tacggtcttt aaaaaggccg taatatccag ctaaacggtc tggttatagg tacattgagc
     4141 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     4201 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     4261 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     4321 ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac
     4381 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cgaagacgaa
     4441 agggcatcgc gcgcggggaa ttcccgggag agctcgatat cgcatgcggt acctctagaa
     4501 gaagcttgga gacaaggtaa aggataaaac agcacaattc caagaaaaac acgatttaga
     4561 acctaaaaag aacgaatttg aactaactca taaccgagag gtaaaaaaag aacgaagtcg
     4621 agatcaggga atgagtttat aaaataaaaa aagcacctga aaaggtgtct ttttttgatg
     4681 gttttgaact tgttctttct tatcttgata catatagaaa taacgtcatt tttattttag
     4741 ttgctgaaag gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg aaaaccctta
     4801 aaattggttg cacagaaaaa ccccatctgt taaagttata agtgactaaa caaataacta
     4861 aatagatggg ggtttctttt aatattatgt gtcctaatag tagcatttat tcagatgaaa
     4921 aatcaagggt tttagtggac aagacaaaaa gtggaaaagt gagaccatgg agagaaaaga
     4981 aaatcgctaa tgttgattac tttgaacttc tgcatattct tgaatttaaa aaggctgaaa
     5041 gagtaaaaga ttgtgctgaa atattagagt ataaacaaaa tcgtgaaaca ggcgaaagaa
     5101 agttgtatcg agtgtggttt tgtaaatcca ggctttgtcc aatgtgcaac tggaggagag
     5161 caatgaaaca tggcattcag tcacaaaagg ttgttgctga agttattaaa caaaagccaa
     5221 cagttcgttg gttgtttctc acattaacag ttaaaaatgt ttatgatggc gaagaattaa
     5281 ataagagttt gtcagatatg gctcaaggat ttcgccgaat gatgcaatat aaaaaaatta
     5341 ataaaaatct tgttggtttt atgcgtgcaa cggaagtgac aataaataat aaagataatt
     5401 cttataatca gcacatgcat gtattggtat gtgtggaacc aacttatttt aagaatacag
     5461 aaaactacgt gaatcaaaaa caatggattc aattttggaa aaaggcaatg aaattagact
     5521 atgatccaaa tgtaaaagtt caaatgattc gaccgaaaaa taaatataaa tcggatatac
     5581 aatcggcaat tgacgaaact gcaaaatatc ctgtaaagga tacggatttt atgaccgatg
     5641 atgaagaaaa gaatttgaaa cgtttgtctg atttggagga aggtttacac cgtaaaaggt
     5701 taatctccta tggtggtttg ttaaaagaaa tacataaaaa attaaacctt gatgacacag
     5761 aagaaggcga tttgattcat acagatgatg acgaaaaagc cgatgaagat ggattttcta
     5821 ttattgcaat gtggaattgg gaacggaaaa attattttat taaagagtag ttcaacaaac
     5881 gggccagttt gttgaagatt agatgctata attgttatta aaaggattga aggatgctta
     5941 ggaagacgag ttattaatag ctgaataaga acggtgctct ccaaatattc ttatttagaa
     6001 aagcaaatct aaaattatct gaaaagggaa tgagaatagt gaatggacca ataataatga
     6061 ctagagaaga aagaatgaag attgttcatg aaattaagga acgaatattg gataaatatg
     6121 gggatgatgt taaggctatt ggtgtttatg gctctcttgg tcgtcagact gatgggccct
     6181 attcggatat tgagatgatg tgtgtcatgt caacagagga agcagagttc agccatgaat
     6241 ggacaaccgg tgagtggaag gtggaagtga attttgatag cgaagagatt ctactagatt
     6301 atgcatctca ggtggaatca gattggccgc ttacacatgg tcaatttttc tctattttgc
     6361 cgatttatga ttcaggtgga tacttagaga aagtgtatca aactgctaaa tcggtagaag
     6421 cccaaacgtt ccacgatgcg atttgtgccc ttatcgtaga agagctgttt gaatatgcag
     6481 gcaaatggcg taatattcgt gtgcaaggac cgacaacatt tctaccatcc ttgactgtac
     6541 aggtagcaat ggcaggtgcc atgttgattg gtctgcatca tcgcatctgt tatacgacga
     6601 gcgcttcggt cttaactgaa gcagttaagc aatcagatct tccttcaggt tatgaccatc
     6661 tgtgccagtt cgtaatgtct ggtcaacttt ccgactctga gaaacttctg gaatcgctag
     6721 agaatttctg gaatgggatt caggagtgga cagaacgaca cggatatata gtggatgtgt
     6781 caaaacgcat accattttga acgatgacct ctaataattg ttaatcatgt tggttacgta
     6841 tttattaact tctcctagta ttagtaatta tcatggctgt catggcgcat taacggaata
     6901 aagggtgtgc ttaaatcggg ccattttgcg taataagaaa aaggattaat tatgagcgaa
     6961 ttgaattaat aataaggtaa tagatttaca ttagaaaatg aaaggggatt ttatgcgtga
     7021 gaatgttaca gtctatcccg gcattgccag tcggggatat taaaaagagt ataggttttt
     7081 attgcgataa actaggtttc actttggttc accatgaaga tggattcgca gttctaatgt
     7141 gtaatgaggt tcggattcat ctatgggagg caagtgatga aggctggcgc tctcgtagta
     7201 atgattcacc ggtttgtaca ggtgcggagt cgtttattgc tggtactgct agttgccgca
     7261 ttgaagtaga gggaattgat gaattatatc aacatattaa gcctttgggc attttgcacc
     7321 ccaatacatc attaaaagat cagtggtggg atgaacgaga ctttgcagta attgatcccg
     7381 acaacaattt gattagcttt tttcaacaaa taaaaagcta aaatctatta ttaatctgtt
     7441 cagcaatcgg gcgcgattgc tgaataaaag atacgagaga cctctcttgt atctttttta
     7501 ttttgagtgg ttttgtccgt tacactagaa aaccgaaaga caataaaaat tttattcttg
     7561 ctgagtctgg ctttcggtaa gctagacaaa acggacaaaa taaaaattgg caagggttta
     7621 aaggtggaga ttttttgagt gatcttctca aaaaatacta cctgtccctt gctgattttt
     7681 aaacgagcac gagagcaaaa cccccctttg ctgaggtggc agagggcagg tttttttgtt
     7741 tcttttttct cgtaaaaaaa agaaaggtct taaaggtttt atggttttgg tcggcactgc
     7801 cgacagcctc gcagagcaca cactttatga atataaagta tagtgtgtta tactttactt
     7861 ggaagtggtt gccggaaaga gcgaaaatgc ctcacatttg tgccacctaa aaaggagcga
     7921 ttta
//
