LOCUS       62056_16440        7215 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      251..299
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      537..992
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1018..1122
                     /label=AmpR promoter
     misc_feature    1307..1686
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     rep_origin      2155..2746
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3034..3055
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        complement(3516..3618)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             4157..5158
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
ORIGIN
        1 catatcctca tatggtcagc atccgccgca gcttcgaagc gtatgtcgat gacatgaata
       61 tcattactgt tctgattcct gctgaacaaa aggaaatcat gacaccgccg gaattcgtcg
      121 acaagcttgg taccagatct gatatccctg cagggatcct ctagagtcga gctcaagcta
      181 gcttggtacg taccagatct gagatcccgg gttctagagg tcgaaattca cctcgaaagc
      241 aagctgataa accgatacaa ttaaaggctc cttttggagc cttttttttt ggagattttc
      301 aacgtgaaaa aattattatt cgcaattcca agctaattca cctcgaaagc aagctgataa
      361 accgatacaa ttaaaggctc cttttggagc cttttttttt ggagattttc aacgtgaaaa
      421 aattattatt cgcaattcca agctctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
      481 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg cagatcacgc
      541 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
      601 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      661 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
      721 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
      781 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
      841 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
      901 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
      961 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     1021 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1081 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1141 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1201 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgac
     1261 tgcaatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1321 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1381 gagcaactcg gtcgccgcat acactattct gcagaatgac ttggttgagt actcaccagt
     1441 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     1501 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1561 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1621 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1681 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1741 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1801 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1861 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1921 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1981 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     2041 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     2101 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2161 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2221 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2281 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     2341 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2401 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2461 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2521 cgaagtactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2581 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2641 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2701 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2761 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2821 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2881 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     2941 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     3001 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     3061 accccaggct ttacacttta tgcttccggc atattctcaa taaacccttt agggaaatag
     3121 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     3181 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     3241 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
     3301 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     3361 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctaaacggt ctggttatag
     3421 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     3481 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     3541 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     3601 cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta
     3661 tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat
     3721 tcgaagacga aagggcatcg cgcgcgggaa attcccggga gagctcgata tcgcatgcgg
     3781 tacctctaga agaagcttgg agacaaggta aaggataaaa cagcacaatt ccaagaaaaa
     3841 cacgatttag aacctaaaaa gaacgaattt gaactaactc ataaccgaga ggtaaaaaaa
     3901 gaacgaagtc gagatcaggg aatgagttta taaaataaaa aaagcacctg aaaaggtgtc
     3961 tttttttgat ggttttgaac ttgttctttc ttatcttgat acatatagaa ataacgtcat
     4021 ttttatttta gttgctgaaa ggtgcgttga agtgttggta tgtatgtgtt ttaaagtatt
     4081 gaaaaccctt aaaattggtt gcacagaaaa accccatctg ttaaagttat aagtgactaa
     4141 acaaataact aaatagatgg gggtttcttt taatattatg tgtcctaata gtagcattta
     4201 ttcagatgaa aaatcaaggg ttttagtgga caagacaaaa agtggaaaag tgagaccatg
     4261 gagagaaaag aaaatcgcta atgttgatta ctttgaactt ctgcatattc ttgaatttaa
     4321 aaaggctgaa agagtaaaag attgtgctga aatattagag tataaacaaa atcgtgaaac
     4381 aggcgaaaga aagttgtatc gagtgtggtt ttgtaaatcc aggctttgtc caatgtgcaa
     4441 ctggaggaga gcaatgaaac atggcattca gtcacaaaag gttgttgctg aagttattaa
     4501 acaaaagcca acagttcgtt ggttgtttct cacattaaca gttaaaaatg tttatgatgg
     4561 cgaagaatta aataagagtt tgtcagatat ggctcaagga tttcgccgaa tgatgcaata
     4621 taaaaaaatt aataaaaatc ttgttggttt tatgcgtgca acggaagtga caataaataa
     4681 taaagataat tcttataatc agcacatgca tgtattggta tgtgtggaac caacttattt
     4741 taagaataca gaaaactacg tgaatcaaaa acaatggatt caattttgga aaaaggcaat
     4801 gaaattagac tatgatccaa atgtaaaagt tcaaatgatt cgaccgaaaa ataaatataa
     4861 atcggatata caatcggcaa ttgacgaaac tgcaaaatat cctgtaaagg atacggattt
     4921 tatgaccgat gatgaagaaa agaatttgaa acgtttgtct gatttggagg aaggtttaca
     4981 ccgtaaaagg ttaatctcct atggtggttt gttaaaagaa atacataaaa aattaaacct
     5041 tgatgacaca gaagaaggcg atttgattca tacagatgat gacgaaaaag ccgatgaaga
     5101 tggattttct attattgcaa tgtggaattg ggaacggaaa aattatttta ttaaagagta
     5161 gttcaacaaa cgggccagtt tgttgaagat tagatgctat aattgttatt aaaaggattg
     5221 aaggatgctt aggaagacga gttattaata gctgaataag aacggtgctc tccaaatatt
     5281 cttatttaga aaagcaaatc taaaattatc tgaaaaggga atgagaatag tgaatggacc
     5341 aataataatg actagagaag aaagaatgaa gattgttcat gaaattaagg aacgaatatt
     5401 ggataaatat ggggatgatg ttaaggctat tggtgtttat ggctctcttg gtcgtcagac
     5461 tgatgggccc tattcggata ttgagatgat gtgtgtcatg tcaacagagg aagcagagtt
     5521 cagccatgaa tggacaaccg gtgagtggaa ggtggaagtg aattttgata gcgaagagat
     5581 tctactagat tatgcatctc aggtggaatc agattggccg cttacacatg gtcaattttt
     5641 ctctattttg ccgatttatg attcaggtgg atacttagag aaagtgtatc aaactgctaa
     5701 atcggtagaa gcccaaacgt tccacgatgc gatttgtgcc cttatcgtag aagagctgtt
     5761 tgaatatgca ggcaaatggc gtaatattcg tgtgcaagga ccgacaacat ttctaccatc
     5821 cttgactgta caggtagcaa tggcaggtgc catgttgatt ggtctgcatc atcgcatctg
     5881 ttatacgacg agcgcttcgg tcttaactga agcagttaag caatcagatc ttccttcagg
     5941 ttatgaccat ctgtgccagt tcgtaatgtc tggtcaactt tccgactctg agaaacttct
     6001 ggaatcgcta gagaatttct ggaatgggat tcaggagtgg acagaacgac acggatatat
     6061 agtggatgtg tcaaaacgca taccattttg aacgatgacc tctaataatt gttaatcatg
     6121 ttggttacgt atttattaac ttctcctagt attagtaatt atcatggctg tcatggcgca
     6181 ttaacggaat aaagggtgtg cttaaatcgg gccattttgc gtaataagaa aaaggattaa
     6241 ttatgagcga attgaattaa taataaggta atagatttac attagaaaat gaaaggggat
     6301 tttatgcgtg agaatgttac agtctatccc ggcattgcca gtcggggata ttaaaaagag
     6361 tataggtttt tattgcgata aactaggttt cactttggtt caccatgaag atggattcgc
     6421 agttctaatg tgtaatgagg ttcggattca tctatgggag gcaagtgatg aaggctggcg
     6481 ctctcgtagt aatgattcac cggtttgtac aggtgcggag tcgtttattg ctggtactgc
     6541 tagttgccgc attgaagtag agggaattga tgaattatat caacatatta agcctttggg
     6601 cattttgcac cccaatacat cattaaaaga tcagtggtgg gatgaacgag actttgcagt
     6661 aattgatccc gacaacaatt tgattagctt ttttcaacaa ataaaaagct aaaatctatt
     6721 attaatctgt tcagcaatcg ggcgcgattg ctgaataaaa gatacgagag acctctcttg
     6781 tatctttttt attttgagtg gttttgtccg ttacactaga aaaccgaaag acaataaaaa
     6841 ttttattctt gctgagtctg gctttcggta agctagacaa aacggacaaa ataaaaattg
     6901 gcaagggttt aaaggtggag attttttgag tgatcttctc aaaaaatact acctgtccct
     6961 tgctgatttt taaacgagca cgagagcaaa accccccttt gctgaggtgg cagagggcag
     7021 gtttttttgt ttcttttttc tcgtaaaaaa aagaaaggtc ttaaaggttt tatggttttg
     7081 gtcggcactg ccgacagcct cgcagagcac acactttatg aatataaagt atagtgtgtt
     7141 atactttact tggaagtggt tgccggaaag agcgaaaatg cctcacattt gtgccaccta
     7201 aaaaggagcg attta
//
