LOCUS       40924_28841       10820 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLucLRH-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10820)
  AUTHORS   Houten SM, Auwerx J.
  TITLE     In vivo imaging of nuclear receptor signaling
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10820)
  AUTHORS   Houten SM, Auwerx J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2004) Institut de Genetique et de Biologie 
            Moleculaire et Cellulaire, 1 rue Laurent Fries, Illkirch 67404, 
            France
REFERENCE   3  (bases 1 to 10820)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10820)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-APR-2004) Institut de Genetique et de Biologie Moleculaire et 
            Cellulaire, 1 rue Laurent Fries, Illkirch 67404, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..40)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      181..636
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        714..818
                     /label=AmpR promoter
     CDS             819..1676
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1850..2438
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2726..2747
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2762..2792
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2800..2816
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2824..2840
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2858..2876
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    2902..4124
                     /label=beta-globin insulator
                     /note="beta-globin insulator"
     misc_feature    4125..5334
                     /label=beta-globin insulator
                     /note="beta-globin insulator"
     protein_bind    5385..5479
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5510..5607
                     /label=LRH-1 binding site
                     /bound_moiety="LRH-1"
     regulatory      5626..6735
                     /label=Wnt-1 promoter
                     /note="Wnt-1 promoter"
                     /regulatory_class="promoter"
     CDS             6777..8426
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(8470..8591)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    8705..10793
                     /label=3' flanking sequence of Wnt-1
                     /note="3' flanking sequence of Wnt-1"
ORIGIN
        1 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
       61 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      121 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      181 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      241 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      301 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      361 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      481 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      601 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
      661 gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa
      721 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      781 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      841 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      901 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      961 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1021 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     1081 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1141 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1201 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1261 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1321 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1381 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1441 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1501 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     1561 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     1621 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1681 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1741 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1801 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1861 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1921 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1981 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2041 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2101 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2161 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2221 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2281 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2341 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2401 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2461 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2521 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2581 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     2641 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2701 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     2761 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     2821 acacaggaaa cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac
     2881 tcaagctatg catccaacgc gcgacgcgtc gactctagag ccccatcctc actgactccg
     2941 tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt cgggctggat
     3001 ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc ccacggaggt
     3061 gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac tgagggacat
     3121 ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag ggcttttcca
     3181 gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc tgcctgtccc
     3241 agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc tgcttgcatc
     3301 cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt ggcctttttg
     3361 tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat cttcttatcc
     3421 ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa cacctgcatg
     3481 tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc cgtgatgcac
     3541 tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc ccccttgata
     3601 ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat atgaggaggc
     3661 agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg ctgtggatgc
     3721 cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc tgggctgctg
     3781 gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc tcctttgcaa
     3841 cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa gctttttccc
     3901 cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga
     3961 tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg
     4021 ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag
     4081 ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagag ccccatcctc
     4141 actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt
     4201 cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc
     4261 ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac
     4321 tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag
     4381 ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc
     4441 tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc
     4501 tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt
     4561 ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat
     4621 cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa
     4681 cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc
     4741 cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc
     4801 ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat
     4861 atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg
     4921 ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc
     4981 tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc
     5041 tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa
     5101 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
     5161 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
     5221 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
     5281 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagag
     5341 ttaactacgt acggccgcgg atccaagctt gcatgcctgc aggtcggagt actgtcctcc
     5401 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag
     5461 cggagtactg tcctccgagc ggagactcta gcgagcgccg gaggcggcca gagcaacctt
     5521 gatggcatgg gcccagagca accttgactg ccccagcggc cagagcaacc ttgatggcat
     5581 gggcccagag caaccttgac tgccccagcg gccgctcgac tcatcacgtc tcctgtcaca
     5641 gccccacctc cttcctgata cactgcgtta agtggtgact gtaacaaaat gacttcatgc
     5701 tctccctgtc ctgagccaaa ttacacaatt atttggaaag ggctcaaaat gttcttcgtt
     5761 agaagtttct ggatacacca atacacagga gcgtgcaccc tcagaacaca tgtacacttt
     5821 gacttaatct cacgggtgac acaccgacgc ttacactccc cctagcccac agaggcaaac
     5881 tgctgggcgc ttctgagttt ctcactgcca ccagctcggt ttgctcagcc tacccccgca
     5941 ccccgcgccc gggaatccct gaccacagct ccacccatgc tctgtctcct tcttttcctt
     6001 ctctgtccag ccgtcggggt tcctgggtga ggaagtgtct ccacggagtc gctggctaga
     6061 accacaactt tcatcctgcc attcagaata gggaagagaa gagaccacag cgtagggggg
     6121 acagaggaga cggacttcga gaggacagcc ccaccggcgc gtgtggggga ggcaatccag
     6181 gctgcaaaca ggttgtcccc agcgcattgt ccccgcgccc cctggcggat gctggtcccc
     6241 gacgggctcc ggacgcgcag aagagtgagg ccggcgcgcg tgggaggcca tcccaagggg
     6301 aggggtcggc ggccagtgca gacctggagg cggggccacc aggcaggggg cgggggtgag
     6361 ccccgacggt tagcctgtca gctctttgct cagaccggca agagccacag cttcgctcgc
     6421 cactcattgt ctgtggccct gaccagtgcg ccctggtgct tttagtgccg cccgggcccg
     6481 gaggggcagc ctcttctcac tgcagtcagc gccgcaacta taagaggcct ataagaggcg
     6541 gtgcctcccg cagtggctgc ttcagcccag cagccaggac agcgaaccat gctgcctgcg
     6601 gcccgcctcc agacttatta gagccagcct gggaactcgc atcactgccc tcaccgctgt
     6661 gtccagtccc accgtcgcgg acagcaacca cagtcgtcag aaccgcagca cagaaccagc
     6721 aaggccaggc aggcctcgcg atagcttggc attccggtac tgttggtaaa gccaccatgg
     6781 aagacgccaa aaacataaag aaaggcccgg cgccattcta tccgctggaa gatggaaccg
     6841 ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt
     6901 ttacagatgc acatatcgag gtggacatca cttacgctga gtacttcgaa atgtccgttc
     6961 ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca
     7021 gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag
     7081 ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg ggcatttcgc
     7141 agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa
     7201 agctcccaat catccaaaaa attattatca tggattctaa aacggattac cagggatttc
     7261 agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg
     7321 tgccagagtc cttcgatagg gacaagacaa ttgcactgat catgaactcc tctggatcta
     7381 ctggtctgcc taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg
     7441 ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc
     7501 cattccatca cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag
     7561 tcgtcttaat gtatagattt gaagaagagc tgtttctgag gagccttcag gattacaaga
     7621 ttcaaagtgc gctgctggtg ccaaccctat tctccttctt cgccaaaagc actctgattg
     7681 acaaatacga tttatctaat ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg
     7741 aagtcgggga agcggttgcc aagaggttcc atctgccagg tatcaggcaa ggatatgggc
     7801 tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg
     7861 tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc
     7921 tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg tccggttatg
     7981 taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag
     8041 acatagctta ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg aagtctctga
     8101 ttaagtacaa aggctatcag gtggctcccg ctgaattgga atccatcttg ctccaacacc
     8161 ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg
     8221 ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg
     8281 tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag
     8341 taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg
     8401 ccaagaaggg cggaaagatc gccgtgtaat tctagagtcg gggcggccgg ccgcttcgag
     8461 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     8521 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     8581 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     8641 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgt
     8701 cgattttggt cgcctctttg gccgagagtt cgtggactcc ggggagaagg ggcgggacct
     8761 acgcttcctc atgaaccttc acaacaacga ggcagggcga acggtacgtc ggtgtgtccg
     8821 gaaccaatgg caggggagat gtaagacagg tgcacgggga cagaggcaca gggaggggct
     8881 tcccgagaga gtgggactct aggagggaag acagagaaga ggtggtggtt gagggcaaag
     8941 aggttcctga gctgatgaca gaacagaaga gattagcagg ctatcaacac gtgggatgta
     9001 ttgagatggc tccatggcac acttttgaaa gataaaagtg acttgctggc gtggagcaga
     9061 gtctggccga atgtccctat ctcagcgggc cattttgcac ttcctctctc ccgagcttag
     9121 tcacacctgg accttggctg aagtttccac agcatcgacg tgacccgggt ggggtggggg
     9181 tggggaagta tgggtggtgg ttcgtgggat gttggctttg accttttctt ccctcctccc
     9241 ctcgtcccct cctcccccag accgtgttct ctgagatgcg ccaagagtgc aaatgccacg
     9301 ggatgtccgg ctcctgcacg gtgcgcacgt gttggatgcg gctgcccacg ctgcgcgctg
     9361 tgggcgacgt gctgcgcgac cgcttcgacg gcgcctcccg cgtcctttac ggcaaccgag
     9421 gcagcaaccg cgcctcgcgg gcggagctgc tgcgcctgga gcccgaagac cccgcgcaca
     9481 agcctccctc ccctcacgac ctcgtctact tcgagaaatc gcccaacttc tgcacgtaca
     9541 gtggccgcct gggcacagct ggcacagctg gacgagcttg caacagctcg tctcccgcgc
     9601 tggacggctg tgagctgctg tgctgtggcc gaggccaccg cacgcgcacg cagcgcgtca
     9661 cggagcgctg caactgcacc ttccactggt gctgccacgt cagctgccgc aactgcacgc
     9721 acacgcgcgt tctgcacgag tgtctatgag gtgccgcgcc tccgggaacg ggaacgctct
     9781 cttccagttc tcagacacac tcgctggtcc tgatgtttgc ccaccctacc gcgtccagcc
     9841 acagtcccag ggttcatagc gatccatctc tcccacctcc tacctgggga ctcctgaaac
     9901 cacttgcctg agtcggctcg aacccttttg ccatcctgag ggccctgacc cagcctacct
     9961 ccctccctct ttgagggaga ctccttttgc actgcccccc aatttggcca gagggtgaga
    10021 gaaagattct tcttctgggg tgggggtggg gaggtcaact cttgaaggtg ttgcggttcc
    10081 tgatgtattt tgcgctgtga cctctttggg tattatcacc tttccttgtc tctcgggtcc
    10141 ctataggtcc cttgagttct ctaaccagca cctctgggct tcaaggcctt tcccctccca
    10201 cctgtagctg aagagtttcc gagttgaaag ggcacggaaa gctaagtggg aaaggaggtt
    10261 gctggaccca gcagcaaaac cctacattct ccttgtctct gcctcggagc cattgaacag
    10321 ctgtgaacca tgcctccctc agcctcctcc caccccttcc tgtcctgcct cctcatcact
    10381 gtgtaaataa tttgcaccga aatgtggccg cagagccacg cgttcggtta tgtaaataaa
    10441 actatttatt gtgctgggtt ccagcctggg ttgcagagac caccctcacc ccacctcact
    10501 gctcctctgt tctgctcgcc agtccttttg ttatccgacc ttttttctct tttacccagc
    10561 ttctcatagg cgcccttgcc caccggatca gtatttcctt ccactgtagc tattagtggc
    10621 tcctcgcccc caccaatgta gtatcttcct ctgaggaata aaatatctat ttttatcaac
    10681 gactctggtc cttgaatcca gaacacagca tggcttccaa cgtcctcttc ccttccaatg
    10741 gacttgcttc tcttctcata gccaaacaaa agagatagag ttgttgaaga tctgtcgacg
    10801 acgtcgggcc caattcgccc
//
