LOCUS       .                      15499 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        238..617
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..820
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2098..2142
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2291..2332
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    2440..2557
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2608..2848
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        4738..4813
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     promoter        4875..5086
                     /label="EF-1α core promoter"
                     /note="core promoter for human elongation factor EF-1α"
     CDS             5151..9251
                     /label="dCas9"
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
     CDS             9306..9326
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             9351..9500
                     /label="VP64"
                     /note="tetrameric repeat of the minimal activation domain
                     of herpes simplex virus VP16 (Beerli et al., 1998)"
     CDS             9522..9578
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             9579..10172
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    10191..10779
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             11304..11484
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    11516..11740
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11786..12214
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        12228..12557
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        12605..12652
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             12671..13042
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    13175..13308
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(13393..13423)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(13438..13459)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(13747..14335)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(14509..15366)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(15367..15471)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg
     2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag
     2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     2821 ttggctttat atatcttgtg gaaaggacga aacaccggag acggttgtaa atgagcacac
     2881 aaaatacaca tgctaaaata ttatattcta tgacctttat aaaatcaacc aaaatcttct
     2941 ttttaataac tttagtatca ataattagaa tttttatgtt cctttttgca aacttttaat
     3001 aaaaatgagc aaaataaaaa aacgctagtt ttagtaactc gcgttgtttt cttcaccttt
     3061 aataatagct actccaccac ttgttcctaa gcggtcagct cctgcttcaa tcattttttg
     3121 agcatcttca aatgttctaa ctccaccagc tgctttaact aaagcattgt ctttaacaac
     3181 tgacttcatt agtttaacat cttcaaatgt tgcacctgat tttgaaaatc ctgttgatgt
     3241 tttaacaaat tctaatccag cttcaacagc tatttcacaa gctttcatga tttcttcttt
     3301 tgttaataaa caattttcca taatacattt aacaacatgt gatccagctg ctttttttac
     3361 agctttcatg tcttctaaaa ctaattcata atttttgtct tttaatgcac caatatttaa
     3421 taccatatca atttctgttg caccatcttt aattgcttca gaaacttcga atgcttttgt
     3481 agctgttgtg catgcaccta gaggaaaacc tacaacattt gttattccta catttgtgcc
     3541 ttttaataat tctttacaat agcttgttca atatgaatta acacaaactg ttgcaaaatc
     3601 aaattcaatt gcttcatcac ataattgttt aatttcagct ttcgtagcat cttgttttaa
     3661 taatgtgtga tctatatatt tgtttagttt cattttttct cctatatatt catttttaat
     3721 tttaattctt taataatttc gtctacttta actttagcgt tttgaacaga ttcaccaaca
     3781 cctataaaat aaatttttag tttaggttca gttccacttg ggcgaacagc aaatcatgac
     3841 ttatcttcta aataaaattt tagtaagtct tgtcctggca tattatacat tccatcgatg
     3901 tagtcttcaa cattaacaac tttaagtcca gcaatttgag ttaagggtgt tgctctcaat
     3961 gatttcatta atggttcaat ttttaatttc ttttcttctg gtttaaaatt caagtttaaa
     4021 gtgaaagtgt aatatgcacc catttcttta aataaatctt ctaaatagtc tactaatgtt
     4081 ttattttgtt ttttataaaa tcaagcagcc tctgctatta atatagaagc ttgtattcca
     4141 tctttatctc tagctgagtc atcaattaca tatccataac tttcttcata agcaaaaaca
     4201 aaatttaatc cgttatcttc ttctttagca atttctctac ccattcattt aaatccagtt
     4261 aaagttttta caatattaac tccatatttt tcatgagcga ttctatcacc caaatcactt
     4321 gttacaaaac ttgaatatag agccggattt tttggaatgc tatttaagcg ttttagattt
     4381 gataattttc aatcaattaa aattggtcct gtttgatttc catctaatct tacaaaatga
     4441 ccatcatgtt ttattgccat tccaaatctg tcagcatctg ggtcattcat aataataata
     4501 tctgcatcat gtttaatacc atattcaagc ggtatttttc atgcaggatc aaattctgga
     4561 tttggattta caacattttt aaatgtttca tcttcaaatg catgctcttc aacctcaata
     4621 acgttatatc ctgattcacg taatattttt ggggtaaatt tagttcctgt tccattaact
     4681 gcgctaaaaa taatttttaa atctttttta gcttcttgct cttttttgta cgtctctgtt
     4741 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     4801 accgagtcgg tgcttttttg aattcgctag ctaggtcttg aaaggagtgg gaattggctc
     4861 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     4921 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     4981 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     5041 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggcgt acggccacca
     5101 tgaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag gacaagaagt
     5161 acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt
     5221 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
     5281 agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga
     5341 agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga
     5401 tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct
     5461 tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg
     5521 aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca
     5581 gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc
     5641 ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt
     5701 tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg
     5761 gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc
     5821 tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggcaacctg attgccctga
     5881 gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc
     5941 agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc
     6001 agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca
     6061 tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat
     6121 acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg
     6181 agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg
     6241 gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg
     6301 gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct
     6361 tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc
     6421 ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga
     6481 ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga
     6541 tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg
     6601 gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg
     6661 agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga
     6721 ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga
     6781 aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga
     6841 aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag
     6901 atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg
     6961 acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac
     7021 tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg
     7081 acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga
     7141 agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt
     7201 ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta
     7261 aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg
     7321 ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg
     7381 acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca
     7441 gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg
     7501 aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc
     7561 agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg
     7621 accaggaact ggacatcaac cggctgtccg actacgatgt ggaccacatc gtgcctcaga
     7681 gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac aaggcccggg
     7741 gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc
     7801 agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga
     7861 gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc
     7921 ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg
     7981 agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg
     8041 atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc
     8101 acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg
     8161 aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga
     8221 gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact
     8281 ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga
     8341 caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga
     8401 aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct
     8461 tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg
     8521 actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg
     8581 tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg
     8641 ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca
     8701 agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg
     8761 agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg
     8821 aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc
     8881 tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact
     8941 acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg
     9001 ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc
     9061 aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca
     9121 agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg
     9181 ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc
     9241 tgggaggcga cagcgctgga ggaggtggaa gcggaggagg aggaagcgga ggaggaggta
     9301 gcggacctaa gaaaaagagg aaggtggcgg ccgctggatc cggacgggct gacgcattgg
     9361 acgattttga tctggatatg ctgggaagtg acgccctcga tgattttgac cttgacatgc
     9421 ttggttcgga tgcccttgat gactttgacc tcgacatgct cggcagtgac gcccttgatg
     9481 atttcgacct ggacatgctg attaactgta caggcagtgg agcaacaaac ttctctctgc
     9541 tgaaacaagc cggagatgtc gaagagaatc ctggaccgac cgagtacaag cccacggtgc
     9601 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     9661 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     9721 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     9781 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     9841 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     9901 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     9961 gagtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
    10021 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
    10081 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
    10141 gcacctggtg catgacccgc aagcccggtg cctgaacgcg ttaagtcgac aatcaacctc
    10201 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
    10261 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
    10321 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
    10381 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
    10441 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
    10501 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
    10561 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
    10621 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
    10681 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
    10741 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
    10801 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
    10861 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
    10921 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
    10981 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
    11041 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
    11101 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
    11161 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
    11221 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
    11281 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
    11341 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    11401 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    11461 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
    11521 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
    11581 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
    11641 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
    11701 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
    11761 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
    11821 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    11881 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    11941 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    12001 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    12061 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    12121 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    12181 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
    12241 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
    12301 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    12361 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
    12421 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    12481 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    12541 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
    12601 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
    12661 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
    12721 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
    12781 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
    12841 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
    12901 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
    12961 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
    13021 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
    13081 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
    13141 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
    13201 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    13261 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
    13321 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    13381 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    13441 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    13501 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    13561 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    13621 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    13681 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    13741 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    13801 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    13861 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    13921 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    13981 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    14041 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    14101 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    14161 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    14221 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    14281 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    14341 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    14401 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    14461 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    14521 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    14581 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    14641 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    14701 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    14761 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    14821 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    14881 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    14941 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    15001 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    15061 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    15121 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    15181 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    15241 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    15301 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    15361 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    15421 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    15481 ccgaaaagtg ccacctgac
//
