LOCUS       V011774                 8922 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011774
VERSION     V011774
KEYWORDS    pLenti6.4 R4R2 V5-DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8922)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1804..1921
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     protein_bind    1950..2074
                     /label="attR4"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2099..2129
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2183..2839
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             3184..3486
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(3530..3654)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3707..3748
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     misc_feature    3772..4360
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     promoter        4379..4878
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     promoter        4892..4939
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             4958..5353
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             5443..5676
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5748..5882
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5909..6044
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6065..6083)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6093..6109)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6251..6706
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6732..6836
                     /label="AmpR promoter"
     CDS             6837..7694
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7868..8456
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8744..8765
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8780..8810
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8818..8834
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8842..8858
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8879..8897
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatgtc gacgttaacg ctagtgatat caactttgta tagaaaagtt gaacgagaaa
     1981 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     2041 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
     2101 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
     2161 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     2221 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     2281 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     2341 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     2401 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     2461 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     2521 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     2581 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     2641 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     2701 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     2761 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     2821 tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta
     2881 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     2941 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     3001 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     3061 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     3121 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     3181 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     3241 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     3301 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     3361 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     3421 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     3481 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     3541 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     3601 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgatat
     3661 ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc
     3721 taaccctctc ctcggtctcg attctacgcg taccggttag taatgatcga caatcaacct
     3781 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3841 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3901 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     3961 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4021 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4081 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4141 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     4201 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4261 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4321 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggcgatgg tacctaccgg
     4381 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     4441 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     4501 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     4561 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     4621 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     4681 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     4741 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     4801 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     4861 tcatctccgg gcctttcgac tctagacacg tgttgacaat taatcatcgg catagtatat
     4921 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagcctt tgtctcaaga
     4981 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga
     5041 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt
     5101 atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc
     5161 ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag
     5221 cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt
     5281 gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta
     5341 tgtgtgggag ggctaagcac aattcgagct cggtaccttt aagaccaatg acttacaagg
     5401 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5461 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     5521 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5581 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5641 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     5701 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     5761 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5821 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     5881 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5941 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     6001 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     6061 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     6121 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     6181 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     6241 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6301 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6361 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6421 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6481 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6541 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6601 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6661 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     6721 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6781 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6841 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6901 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6961 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7021 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7081 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7141 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     7201 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     7261 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7321 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7381 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7441 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     7501 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7561 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7621 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7681 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7741 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7801 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7861 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7921 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7981 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8041 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8101 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8161 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8221 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8281 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8341 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8401 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8461 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8521 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     8581 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     8641 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     8701 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     8761 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     8821 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     8881 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc tt
//
