LOCUS       V014062                 9318 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014062
VERSION     V014062
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             5..185
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    257..391
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      418..553
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(574..592)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(602..618)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      760..1215
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1241..1345
                     /label="AmpR promoter"
     CDS             1346..2203
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2377..2965
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    3253..3274
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3289..3319
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3327..3343
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3351..3367
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3388..3406
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3434..3660
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    3888..4013
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    4506..4739
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             4924..4968
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             5117..5158
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    5235..5351
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        5374..5677
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5678..5881
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             5977..7404
                     /label="tdTomato"
                     /note="tandem dimeric (pseudo-monomeric) derivative of
                     DsRed (Shaner et al., 2004)"
     promoter        7420..7919
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             7940..8536
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    8546..9134
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
ORIGIN
        1 cntggggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
       61 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      121 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      181 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
      241 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
      301 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
      361 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
      421 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
      481 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
      541 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
      601 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      661 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      721 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
      781 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      841 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      901 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      961 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     1021 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     1081 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     1141 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     1201 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     1261 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     1321 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     1381 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     1441 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1501 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1561 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1621 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1681 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1741 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1801 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1861 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     1921 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1981 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     2041 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     2101 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     2161 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     2221 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     2281 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2341 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2401 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2461 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2521 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2581 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2641 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2701 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2761 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2821 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2881 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2941 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3001 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3061 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3121 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     3181 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     3241 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     3301 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     3361 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     3421 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     3481 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     3541 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     3601 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     3661 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     3721 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     3781 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     3841 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     3901 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     3961 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     4021 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     4081 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     4141 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     4201 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     4261 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     4321 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     4381 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     4441 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     4501 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     4561 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     4621 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     4681 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     4741 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     4801 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     4861 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     4921 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     4981 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     5041 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     5101 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     5161 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     5221 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     5281 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     5341 ttcaaaattt tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc
     5401 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     5461 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     5521 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     5581 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     5641 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     5701 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     5761 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     5821 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     5881 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     5941 aagacaccga ctctagagga tccaccggtc gccaccatgg tgagcaaggg cgaggaggtc
     6001 atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc
     6061 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     6121 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac
     6181 ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc
     6241 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg
     6301 acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc
     6361 aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc
     6421 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg
     6481 aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg
     6541 caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac
     6601 tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt cctggggcat
     6661 ggcaccggca gcaccggcag cggcagctcc ggcaccgcct cctccgagga caacaacatg
     6721 gccgtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac
     6781 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     6841 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc
     6901 atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg
     6961 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg
     7021 accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc
     7081 ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     7141 tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg
     7201 aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag
     7261 cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac
     7321 gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg
     7381 tacggcatgg acgagctgta caagtaggat aattctaccg ggtaggggag gcgcttttcc
     7441 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
     7501 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     7561 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     7621 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     7681 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     7741 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     7801 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     7861 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     7921 cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     7981 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     8041 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     8101 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     8161 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     8221 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     8281 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     8341 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     8401 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     8461 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     8521 ccgcaagccc ggtgcctgag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     8581 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     8641 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     8701 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     8761 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     8821 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     8881 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     8941 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     9001 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     9061 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     9121 ggccgcctcc ccgcctggga attcgagctc ggtaccttta agaccaatga cttacaaggc
     9181 agctgtagat cttagccact ttttaaaaga aaaagggggg actggaaagg gctaattcac
     9241 tcccaacgaa gacagatctg ctttttgctt gtactgggtc tctctgggtt agaccagatc
     9301 tgagcctggg gagctctc
//
