LOCUS       .                       7240 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        196..224
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             272..1459
                     /label="TcR"
                     /note="tetracycline efflux protein"
     promoter        complement(1637..1655)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1660..1676)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(1751..2773)
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     promoter        complement(2843..2947)
                     /label="AmpR promoter"
     rep_origin      3526..4114
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        4314..4344
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4352..4368
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     4376..4392
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             4412..5983
                     /gene="nifA"
                     /label="nifA"
                     /note="Nif-specific regulatory protein from Klebsiella
                     pneumoniae. Accession#: P03027"
     promoter        6028..6056
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     terminator      complement(7007..7034)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(7126..7212)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 aattaattct catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca
      241 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat
      301 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
      361 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
      421 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
      481 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
      541 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg
      601 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
      661 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
      721 cgggggactg ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa
      781 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg
      841 acccatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
      901 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
      961 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     1021 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     1081 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     1141 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     1201 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     1261 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
     1321 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     1381 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     1441 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
     1501 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga
     1561 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
     1621 agaaaataaa atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     1681 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     1741 tgcctcccta ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga
     1801 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
     1861 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
     1921 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
     1981 acgcccggag ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
     2041 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg
     2101 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca
     2161 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg
     2221 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
     2281 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
     2341 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
     2401 ccgcagcgat cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt
     2461 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
     2521 cgctgaattc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
     2581 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
     2641 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
     2701 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
     2761 caggcttttt catctgggcg ccagcactca gcgagaagag actcagtgcg cccaatgcca
     2821 gggtgatttt tgcaatcttc atactcttcc tttttcaata ttattgaagc atttatcagg
     2881 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2941 ttccgcgtgc ggccgctcct tcaggcactg ccgacgacag cccaggcgga ggtcctgagc
     3001 gccggcgcca aatttgcata aagagctacc cagaagccct cgcgcgcgga aacgggcaaa
     3061 aaagggcttc taatatggaa atattacgcc gaatcgcgtc acaaattggc taagcgggcc
     3121 taagagttaa caagatgtgc tattaagcgg agccttttgg tgggaagaaa cggaacagtc
     3181 actgtgttct aaaagaactt gcaggatgag cctttaaata ccgcagtctc gacgctctta
     3241 gtcggagacg ttgatttcgt ctctgtttaa gagctatgct ggaaacagca tagcaagttt
     3301 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttgttt
     3361 tccatatttt tttcgcattg ctttccttgg cttttacacc cttacgcagc ctcgccccct
     3421 tttcccaggg agaccctcga gccacccatg accaaaatcc cttaacgtga gttacgcgtc
     3481 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3541 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3601 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3661 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3721 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3781 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3841 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3901 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3961 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4021 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4081 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4141 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4201 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4261 cgagcgcagc gagtcagtga gcgaggaagc ggaagagctt aggcacccca ggctttacac
     4321 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     4381 aacagctatg accatgatta cgccaagcgc gatgatccat aaatccgatt cggacaccac
     4441 cgtcaggcgc ttcgatctct cccagcagtt taccgccatg cagcggataa gcgtggtgct
     4501 gagccgcgcc accgaagcga gcaaaaccct gcaggaggtg ctgagcgttt tacataacga
     4561 tgcgtttatg cagcacggga tgatttgcct gtacgacagc cagcaggaga tcctgagcat
     4621 cgaggcgctg cagcaaacgg aaaaccagac gctgcccggc agtacgcaaa ttcgctaccg
     4681 gccgggagaa ggactggtcg gtaccgtgct ggcgcagggc cagtcgctgg tgctgccgcg
     4741 cgtcgccgat gaccagcgct ttctcgatcg tctgagcctg tacgactacg acctgccgtt
     4801 tatcgccgtt ccgctgatgg gcccccattc ccggcccatc ggcgtactgg cggcgcagcc
     4861 gatggcgcgt caggaagagc ggctacccgc ctgcacgcgc tttctcgaaa ccgtcgccaa
     4921 tctggtggcc cagacgattc gcctgatgat tctgccgacc tccgccccgc agccgccgca
     4981 gcagagcccc cgagttgagc gcccgcgcgc ctgtacccct tcacgcggtt tcggcctgga
     5041 gaatatggtc ggcaaaagcc cggcgatgcg gcagattatg gacattatcc gtcaggtctc
     5101 ccgctgggat accacggtgc tggtgcgcgg cgagagcggc accgggaaag agctgatcgc
     5161 caacgccatt catcataatt ccccgcgcgc cgccgccgca ttcgtcaaat ttaactgcgc
     5221 ggcactgccg gacaatctgc tggagagcga gctgttcggc catgagaaag gcgcgtttac
     5281 cggcgcggtg cgccagcgga aaggccgctt tgagctggcg gacggcggca ccctattcct
     5341 ggatgaaatc ggtgaaagca gcgcctcgtt tcaggccaag ctgctgcgaa ttctgcaaga
     5401 aggagagatg gagcgcgtcg gcggcgatga aaccctgcgc gtcaacgtac gcatcatcgc
     5461 ggcgaccaac cgccatctgg aagaggaggt gcggctgggg catttccgcg aagaccttta
     5521 ctaccgcctg aacgtaatgc ctatcgcgct gccgccgctg cgcgagcgcc aggaggatat
     5581 cgccgaactg gcgcattttc tggtacgtaa aatcgcccac agccaggggc gaacgctgcg
     5641 catcagcgat ggggcaattc gcctgctgat ggagtacagc tggccgggaa acgtccgcga
     5701 actggaaaac tgtctcgaac gttcggcggt gatgtcggaa agcggcctga tcgaccggga
     5761 cgtgattctg ttcaaccatc gcgataaccc gccgaaagcg ctacccaacg gcggccagac
     5821 tgaagacggc tggctcgaca acagcctcga cgagcgccag cggctgattg ccgccctgga
     5881 gaaggcgggc tgggtgcagg ccaaagcggc gcggctgctc gggatgacgc cgcgtcaggt
     5941 ggcgtatcgc attcagatca tggatatcac catgccgcgg ctgtgaatct cgacgctctc
     6001 ccttatgcga ctcctgcatt aggaaatttg acaattaatc atcggctcgt ataatgtgtg
     6061 gaatgtctca tgagcggata tttcacacag gaaacagtat tcatgaagaa atgggctact
     6121 ctgtgcctga tcggtctggc tgccctgtct gtaggtcaga aggctctgtc tcgacatcct
     6181 ttagtgatgg gtaactggaa actgaacggc agccgccaca tggtaaacga actggtcgcg
     6241 aacctgcgta ccgaactggc tggcgtaacc ggctgtgcag ttgccattgc tccgcctgag
     6301 atgtacatcg acctggctaa acaagccgct gcgggcagcc acattcacct gggcgcgcag
     6361 aacgttgacc tgaacctgtc cggcgcgttc actggcgaaa cctctgccga aatgctgaaa
     6421 gacatcggcg ctgaatacat catcatcggc cactccgagc gccgtaccta ccacaaagag
     6481 tccgacgagt ttatcgccag gaaattcgcg gtactgaaag agcagggtct gatcccggtt
     6541 ctgtgcatcg gtgaaaccga agcggaaaac gaagcgggca aaacggaaga agtgtgcgcg
     6601 cgtcagatcg acgccgttct gaaaacccag ggcgcggcgg cgttcgaagg cgcagttatc
     6661 gcttatgaac ccgtatgggc gatcggtacc ggcaaatccg ctaccccggc tcaggctcag
     6721 gctgtacaca aattcattcg cgaccacatc gctaaagctg atgcgaaagt ggctgaacaa
     6781 gtgatcatcc agtacggcgg ttccgttaac gcgggcaacg cagcagagct gttcacccag
     6841 ccggacatcg acggcgcgct ggttggcggc gcatccctga aagctgacgc tttcgcggtg
     6901 atcgttaaag cagcagaagc cgcgaaaaaa gcgtaagggg gtcctctaga gcggtgcatg
     6961 gatccaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     7021 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     7081 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     7141 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     7201 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt
//
