LOCUS       40924_29551        3993 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLZ41, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Zhou L, Zhang K, Wanner BL.
  TITLE     Chromosomal expression of foreign and native genes from regulatable 
            promoters in Escherichia coli
  JOURNAL   Methods Mol. Biol. 267, 123-134 (2004)
  PUBMED    15269420
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Zhou L, Zhang K, Wanner BL.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2003) Biological Sciences, Purdue University, 
            Lilly Hall, West Lafayette, IN 47907, USA
REFERENCE   3  (bases 1 to 3993)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3993)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Mol. Biol. 267, 123-134 (2004)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-FEB-2003) Biological Sciences, Purdue University, Lilly Hall, 
            West Lafayette, IN 47907, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      50..136
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      228..255
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        425..543
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     promoter        581..685
                     /label=AmpR promoter
     CDS             686..1543
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1634..1880
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     protein_bind    1894..2211
                     /label=phage Phi-80 attP
                     /note="attachment site of phage phi-80"
     rep_origin      complement(2322..2710)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(2861..3517)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPKFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(3518..3620)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     misc_feature    3817..3993
                     /label=oriT2
                     /note="oriT2"
ORIGIN
        1 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg
       61 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
      121 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg
      181 gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct
      241 gacggatggc ctttttgcgt ggccagtgcc aagcttgcat gcggcctcct gatgtcgtca
      301 acacggcgaa atagtaatca cgaggtcagg ttcttacctt aaattttcga cggaaaacca
      361 cgtaaaaaac gtcgattttt caagatacag cgtgaatttt caggaaatgc ggtgagcatc
      421 acatcaccac aattcagcaa attgtgaaca tcatcacgtt catctttccc tggttgccaa
      481 tggcccattt tcctgtcagt aacgagaagg tcgcgaattc aggcgctttt tagactggtc
      541 gtaatgaaat tcagcaggat cacactagtc atatgtgaag agcggaaccc ctatttgttt
      601 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      661 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      721 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      781 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
      841 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
      901 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
      961 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1021 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1081 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1141 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1201 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt
     1261 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1321 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1381 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1441 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1501 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt taacgaagag
     1561 cggatccccg ggtaccgagc tcgaattctc atgtttgaca gcttatcact gatcagtgaa
     1621 ttaatggcga tgacgcatcc tcacgataat atccgggtag gcgcaatcac tttcgtctct
     1681 actccgttac aaagcgaggc tgggtatttc ccggcctttc tgttatccga aatccactga
     1741 aagcacagcg gctggctgag gagataaata ataaacgagg ggctgtatgc acaaagcatc
     1801 ttctgttgag ttaagaacga gtatcgagat ggcacatagc cttgctcaaa ttggaatcag
     1861 gtttgtgcca ataccagtag aaacagacga agaagctagc tgataacggg aaagaaacag
     1921 agaagggcac aaatattgtg tactttaatg tgccctttaa tttattgatt ggtggttgaa
     1981 ttgtccgtaa ctttttgatt taagtgcaaa tttctaataa attagaacac tttcttaaat
     2041 tgtcatttgg catattacga acaattccgc gtaaaaacgt tctgttacgc taaaccctta
     2101 tccagcaggc tttcaaggat gtaaaccata acactctgcg aactagtgtt acattgcgtg
     2161 tagctttgag tgggcaactt tgtgtacact tttgtgtacc caaaaacaaa aatgtgtacc
     2221 cattcaatga tcaccgacac aaagctcagg aaggcgctcg gcaagaaaag agatgatatc
     2281 gagattattt ctgattcgca cgggcccatg gctaattccc atgtcagccg ttaagtgttc
     2341 ctgtgtcact gaaaattgct ttgagaggct ctaagggctt ctcagtgcgt tacatccctg
     2401 gcttgttgtc cacaaccgtt aaaccttaaa agctttaaaa gccttatata ttcttttttt
     2461 tcttataaaa cttaaaacct tagaggctat ttaagttgct gatttatatt aattttattg
     2521 ttcaaacatg agagcttagt acgtgaaaca tgagagctta gtacgttagc catgagagct
     2581 tagtacgtta gccatgaggg tttagttcgt taaacatgag agcttagtac gttaaacatg
     2641 agagcttagt acgtgaaaca tgagagctta gtacgtacta tcaacaggtt gaactgctga
     2701 tcttcagatc ctctacgccg gacgcatcgt ggccggatct tgcggccgca aaaattaaaa
     2761 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2821 cttaatcagt gaggcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat
     2881 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca cagacggcat
     2941 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     3001 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     3061 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     3121 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     3181 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     3241 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga
     3301 atttcggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     3361 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     3421 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     3481 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     3541 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     3601 aacctcttac gtgccgatca acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg
     3661 tatcaacagg gacaccagga tttatttatt ctgcgaagtg atcttccgtc acaggtattt
     3721 attcggcgca aagtgcgtcg ggtgatgctg ccaacttact gatttagtgt atgatggtgt
     3781 ttttgaggtg ctccagtggc ttctgtttct atcagcatcg atgattatag cgattttttc
     3841 ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt
     3901 tccttggtgt atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag
     3961 cgggtgttcc ttcttcactg tcccttattc gca
//
