LOCUS       V013844                 9361 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013844
VERSION     V013844
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2185..3396
                     /label="UbC promoter"
                     /note="human ubiquitin C promoter"
     CDS             3416..4123
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             4202..4597
                     /label="BSD"
                     /note="blasticidin S deaminase"
     enhancer        4631..5010
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5011..5214
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        5240..5258
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    5329..5917
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6124..6757
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6886..6902)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6910..6926)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6934..6964)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6979..7000)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7288..7876)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8050..8907)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8908..9012)
                     /label="AmpR promoter"
     polyA_signal    9060..9194
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatggcctc cgcgccgggt tttggcgcct cccgcgggcg
     2221 cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc
     2281 cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc
     2341 agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct
     2401 ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat
     2461 ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag
     2521 ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact
     2581 tggtgagtag cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg
     2641 gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc
     2701 ctgaactggg ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga
     2761 agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc
     2821 aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg
     2881 gctggggcac catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt
     2941 gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt
     3001 gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg
     3061 tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg
     3121 ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa
     3181 gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc
     3241 cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct
     3301 tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc
     3361 gctaaattct ggccgttttt ggcttttttg ttagactcga gcggccgccg ccaccatggt
     3421 gagcaagggc gaggagaata acatggccat catcaaggag ttcatgcgct tcaaggtgcg
     3481 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     3541 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     3601 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     3661 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     3721 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     3781 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     3841 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     3901 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     3961 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     4021 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     4081 cgagggccgc cactccaccg gcggcatgga cgagctgtac aaggtcgagg gaagcggagc
     4141 tactaacttc agcctgctga agcaggctgg agacgtggag gagaaccctg gacctgtcga
     4201 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     4261 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     4321 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     4381 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     4441 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     4501 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     4561 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa tctagacatg tccaatatga
     4621 ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     4681 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     4741 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     4801 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     4861 gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa
     4921 tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac
     4981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg
     5041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     5101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg
     5161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
     5221 gtgaaccgtc agaattttgt aatacgactc actatagggc ggccgggaat tcgtcgactg
     5281 gatcccggga tacgcgtcga tgcataccgg tcctaggcgc gccggaacaa tcaacctctg
     5341 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     5401 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     5461 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5521 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     5581 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     5641 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     5701 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     5761 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     5821 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     5881 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag
     5941 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     6001 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     6061 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg
     6121 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     6181 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     6241 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     6301 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     6361 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     6421 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     6481 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     6541 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     6601 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6661 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6721 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     6781 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc
     6841 tagcgtttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     6901 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6961 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7021 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7081 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7141 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7201 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7261 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7321 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7381 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7441 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7501 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7561 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7621 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7681 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7741 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7801 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7861 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7921 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7981 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8041 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8101 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8161 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8221 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8281 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8341 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8401 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8461 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8521 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8581 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8641 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8701 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8761 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8821 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8881 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8941 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9001 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca
     9061 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     9121 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     9181 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
     9241 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
     9301 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
     9361 t
//
