LOCUS       .                      10090 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             complement(2504..3532)
                     /label="Cre"
                     /note="site-specific recombinase"
     promoter        complement(3592..3959)
                     /label="TRE3GS promoter"
                     /note="3rd-generation Tet-responsive promoter that can be
                     activated by binding of Tet-On® 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription
                     factors"
     promoter        3976..4486
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             4505..5248
                     /label="Tet-On® 3G"
                     /note="modified rtTA protein that binds tightly to
                     promoters containing the tet operator in the presence of
                     doxycycline"
     promoter        5262..5591
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5600..5995
                     /label="BSD"
                     /note="blasticidin S deaminase"
     misc_feature    6012..6600
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6808..7441
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7618..7648)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7663..7684)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7972..8557)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8731..9588)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(9589..9693)
                     /label="AmpR promoter"
     polyA_signal    9741..9875
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat
     2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg
     2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg
     2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca
     2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat
     2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc ctaatcgcca tcttccagca
     2521 ggcgcaccat tgcccctgtt tcactatcca ggttacggat atagttcatg acaatattta
     2581 cattggtcca gccaccagct tgcatgatct ccggtattga aactccagcg cgggccatat
     2641 ctcgcgcggc tccgacacgg gcactgtgtc cagaccaggc caggtatctc tgaccagagt
     2701 catccttagc gccgtaaatc aatcgatgag ttgcttcaaa aatcccttcc agggcgcgag
     2761 ttgatagctg gctggtggca gatggcgcgg caacaccatt ttttctgacc cggcaaaaca
     2821 ggtagttatt cggatcatca gctacaccag agacggaaat ccatcgctcg accagtttag
     2881 ttacccccag gctaagtgcc ttctctacac ctgcggtgct aaccagcgtt ttcgttctgc
     2941 caatatggat taacattctc ccaccgtcag tacgtgagat atctttaacc ctgatcctgg
     3001 caatttcggc tatacgtaac agggtgttat aagcaatccc cagaaatgcc agattacgta
     3061 tatcctggca gcgatcgcta ttttccatga gtgaacgaac ctggtcgaaa tcagtgcgtt
     3121 cgaacgctag agcctgtttt gcacgttcac cggcatcaac gttttctttt cggatccgcc
     3181 gcataaccag tgaaacagca ttgctgtcac ttggtcgtgg cagcccggac cgacgatgaa
     3241 gcatgtttag ctggcccaaa tgttgctgga tagtttttac tgccagaccg cgcgcctgaa
     3301 gatatagaag ataatcgcga acatcttcag gttctgcggg aaaccatttc cggttattca
     3361 acttgcacca tgccgcccac gaccggcaaa cggacagaag cattttccag gtatgctcag
     3421 aaaacgcctg gcgatccctg aacatgtcca tcaggttctt gcgaacctca tcactcgttg
     3481 catcgaccgg taatgcaggc aaattttggt gtacggtcag taaattggac accttcctct
     3541 tcttcttggg catggtggcg gatccgccgg caccggtgta tacgggaatt ctttacgagg
     3601 gtaggaagtg gtacggaaag ttggtataag acaaaagtgt tgtggaattg aagtttactc
     3661 aaaaaatcag cactctttta taggcgccct ggtttacata agcaaagctt atacgttctc
     3721 tatcactgat agggagtaaa ctggatatac gttctctatc actgataggg agtaaactgt
     3781 agatacgttc tctatcactg atagggagta aactggtcat acgttctcta tcactgatag
     3841 ggagtaaact ccttatacgt tctctatcac tgatagggag taaagtctgc atacgttctc
     3901 tatcactgat agggagtaaa ctcttcatac gttctctatc actgataggg agtaaactcg
     3961 aggtgataat tccacggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca
     4021 gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg
     4081 cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc
     4141 cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg
     4201 ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca
     4261 gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg
     4321 gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt
     4381 gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc
     4441 ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg atcatcgaat
     4501 taccatgtct agactggaca agagcaaagt cataaactct gctctggaat tactcaatgg
     4561 agtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
     4621 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat
     4681 gctggacagg catcataccc actcctgccc cctggaaggc gagtcatggc aagactttct
     4741 gcggaacaac gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt
     4801 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
     4861 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
     4921 ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga
     4981 gacacctacc accgattcta tgcccccact tctgaaacaa gcaattgagc tgttcgaccg
     5041 gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
     5101 acagctaaag tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat
     5161 gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga
     5221 cgattttgac cttgacatgc tccccgggta aacgcgcgaa tgtgtgtcag ttagggtgtg
     5281 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5341 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5401 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     5461 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5521 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5581 gcttttgcaa aacgcgacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga
     5641 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc
     5701 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
     5761 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
     5821 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
     5881 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
     5941 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaaac
     6001 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6061 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6121 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6181 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6241 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6301 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6361 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     6421 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6481 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6541 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6601 ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     6661 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     6721 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     6781 gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag
     6841 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     6901 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     6961 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     7021 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     7081 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     7141 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     7201 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     7261 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7321 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7381 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7441 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     7501 gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg acctctagct agagcttggc
     7561 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7621 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7681 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7741 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7801 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7861 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7921 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7981 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8041 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8101 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8161 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8221 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8281 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8341 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8401 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8461 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg
     8521 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8581 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8641 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8701 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8761 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8821 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8881 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8941 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9001 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9061 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9121 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9181 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9241 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9301 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9361 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9421 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9481 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9541 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9601 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9661 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9721 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca
     9781 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9841 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
     9901 atcatcccaa acttcccacc ccatacccta ttaccactgc caattaccta gtggtttcat
     9961 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
    10021 atatgtacta caaacttagt agtttttaaa gaaattgtat ttgttaaata tgtactacaa
    10081 acttagtagt
//
