LOCUS V038793 8548 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038793 VERSION V038793 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..3244 /label="Zfp593(NM_024215)" /note="Zfp593(NM_024215)" /gene="Zfp593" CDS 3251..3316 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 3336..3835 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 3856..4455 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 4469..5057 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5264..5897 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6026..6042) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6050..6066 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6074..6104) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6119..6140 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6428..7016) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7187..8047) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8048..8152) /gene="bla" /label="AmpR promoter" polyA_signal 8200..8334 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgggtcg ctctcggcgg accggcgcgc accgagcaca 2881 ttccttagcc cgccagatga aggccaagaa gcggcggccg gacctggatg agattcatcg 2941 cgagctgagg ccgcaggggc tcccgcggcc caagccggag ccggacgcgg agcccgaccc 3001 ggacctgcca gggggcggcc tgcatcgctg tctggcttgc gcgaggtact tcatcgattc 3061 agccaacctg aagacccatt tccgatccaa agaccacaag aaaaggctga agcagctgag 3121 tgtcgaaccc tacagtcagg aagaagctga gagggcagcg ggcatgggct cgtatgttca 3181 accccagcgg ctgggcgtgc ccacagaagt gtccactgat atccctgaga tggacacgtc 3241 caccggatcc gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa 3301 ggatgacgat gacaagtagt ctagataatt ctaccgggta ggggaggcgc ttttcccaag 3361 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct 3421 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg 3481 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct 3541 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga 3601 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg 3661 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg 3721 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct 3781 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg 3841 cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 3901 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 3961 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 4021 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 4081 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 4141 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 4201 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 4261 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 4321 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 4381 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 4441 aagcccggtg cctgaccgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag 4501 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat 4561 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc 4621 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg 4681 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct 4741 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct 4801 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg 4861 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac 4921 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct 4981 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct 5041 ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca 5101 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag 5161 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag 5221 gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac 5281 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct 5341 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc 5401 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc 5461 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag 5521 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac 5581 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg 5641 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct 5701 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 5761 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 5821 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 5881 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 5941 aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttta ccgtcgacct 6001 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 6061 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 6121 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 6181 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 6241 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 6301 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 6361 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 6421 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 6481 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 6541 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 6601 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 6661 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 6721 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 6781 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 6841 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 6901 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 6961 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 7021 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 7081 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 7141 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 7201 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 7261 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 7321 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 7381 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 7441 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 7501 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 7561 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 7621 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 7681 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 7741 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 7801 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 7861 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 7921 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 7981 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 8041 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 8101 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 8161 cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat 8221 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 8281 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg 8341 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc 8401 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa 8461 agaaattgta tttgttaaat atgtactaca aacttagtag tttttaaaga aattgtattt 8521 gttaaatatg tactacaaac ttagtagt //