LOCUS V028546 9972 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V028546 VERSION V028546 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2830..2856 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2863..4728 /label="SLC24A4(NM_153646)" /note="SLC24A4(NM_153646)" /gene="SLC24A4" promoter 4760..5259 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5280..5879 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5893..6481 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6688..7321 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7450..7466) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7474..7490 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7498..7528) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7543..7564 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7852..8440) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8611..9471) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9472..9576) /gene="bla" /label="AmpR promoter" polyA_signal 9624..9758 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 gccaccatgt acccatacga cgtaccagat tacgctgaat tcatggcgct ccgcgggacc 2881 ctccggccgc tcaaagttcg caggaggcga gagatgctgc cgcagcaagt cggcttcgtg 2941 tgcgcggtgc tggccctggt gtgctgtgcg tccggcctct tcggcagctt ggggcacaaa 3001 acagcttctg ctagcaaacg tgtcctgcca gacacgtgga gaaatagaaa gttgatggcc 3061 ccagtgaatg ggacacagac agccaagaac tgcacagatc ctgcgattca cgagttcccc 3121 acagatctgt tctccaataa ggagcgacag cacggagccg tcctgctgca catccttggt 3181 gctctgtata tgttctatgc cttggccata gtgtgcgatg acttctttgt tccgtctcta 3241 gagaagatct gtgagagact ccatctgagc gaagatgtgg ctggagccac cttcatggct 3301 gcaggaagct caacgccaga gctgtttgcg tctgttattg gggtgttcat cacccatggg 3361 gacgtcgggg tgggcaccat cgtgggctct gctgtgttca acatcctgtg cataattgga 3421 gtgtgcggac tgtttgctgg ccaggtggtc cgtctgacgt ggtgggccgt gtgccgagac 3481 tccgtgtact acaccatctc tgtcatcgtg ctcatcgtgt tcatatatga tgaacaaatt 3541 gtgtggtggg aaggcctggt gctcatcatc ttgtatgtgt tttatattct gatcatgaag 3601 tacaatgtga agatgcaagc ctttttcaca gtcaaacaaa agagcattgc aaacggtaac 3661 ccggtcaaca gtgagctgga ggctggtaat gatttctatg acggtagcta tgatgaccct 3721 tccgtgccat tgctggggca agtgaaggag aagccacagt atggcaagaa ccccgtggtg 3781 atggtggacg agattatgag ctccagccct cccaagttca ccttccctga agcaggctta 3841 cgaatcatga tcaccaataa gtttggaccc aggacccgac tacggatggc cagcaggatc 3901 atcattaatg agcggcagag actgatcaac tcggccaatg gtgtgagcag taagccgctt 3961 caaaacggga ggcacgagaa cattgagaac gggaatgttc ctgtggaaaa ccccgaagac 4021 cctcagcaga atcaggagca gcagccgccg ccacagccac caccgccaga gccagagccg 4081 gtggaggctg acttcctgtc ccccttctcc gtgccggagg ccagagggga caaggtcaag 4141 tgggtgttca cctggcccct catcttcctc ctgtgcgtca ccattcccaa ctgcagcaag 4201 ccccgctggg agaagttctt catggtcacc ttcatcaccg ccacgctgtg gatcgctgtg 4261 ttctcctaca tcatggtgtg gctggtgact attatcggat acacacttgg gatcccggat 4321 gtcatcatgg gcattacttt cctggcagca gggacaagtg ttccagactg catggccagc 4381 ctaattgtgg cgagacaagg ccttggggac atggcagtct ccaacaccat aggaagcaac 4441 gtgtttgaca tcctggtagg acttggtgta ccgtggggcc tgcagaccat ggttgttaat 4501 tatggatcaa cagtgaagat caacagccgg gggctggtct attccgtggt cctgttgctg 4561 ggctctgtcg ctctcaccgt cctcggcatc cacctaaaca agtggcgact ggaccggaag 4621 ctgggtgtct acgtgctggt tctctacgcc atcttcttgt gcttctccat aatgatagag 4681 tttaacgtct ttaccttcgt caacttgccg atgtgccggg aagacgatta gggatcccgc 4741 gactctagat aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc 4801 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat 4861 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct 4921 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg 4981 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa 5041 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg 5101 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg 5161 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct 5221 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca 5281 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac 5341 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc 5401 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca 5461 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga 5521 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt 5581 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc 5641 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca 5701 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg 5761 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg 5821 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac 5881 cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 5941 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 6001 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 6061 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 6121 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 6181 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 6241 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt 6301 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 6361 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 6421 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 6481 cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata 6541 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt 6601 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta 6661 gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag 6721 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca 6781 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag 6841 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg 6901 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc 6961 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat 7021 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg 7081 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact 7141 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 7201 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 7261 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 7321 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca 7381 gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg acctctagct agagcttggc 7441 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 7501 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 7561 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 7621 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 7681 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 7741 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 7801 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 7861 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 7921 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 7981 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 8041 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 8101 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 8161 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 8221 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 8281 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 8341 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 8401 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 8461 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 8521 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 8581 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 8641 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 8701 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 8761 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 8821 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 8881 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 8941 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 9001 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 9061 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 9121 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 9181 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 9241 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 9301 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 9361 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 9421 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 9481 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 9541 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 9601 tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa 9661 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 9721 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc 9781 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc 9841 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt 9901 aaatatgtac tacaaactta gtagttttta aagaaattgt atttgttaaa tatgtactac 9961 aaacttagta gt //