LOCUS V018956 9355 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018956 VERSION V018956 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2827..4080 /label="SERPINA1(NM_000295)" /note="SERPINA1(NM_000295)" /gene="SERPINA1" promoter 4143..4642 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 4663..5262 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5276..5864 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6071..6704 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6833..6849) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6857..6873 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6881..6911) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6926..6947 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7235..7823) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7994..8854) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8855..8959) /gene="bla" /label="AmpR promoter" polyA_signal 9007..9141 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 gccaccatgc cgtcttctgt ctcgtggggc atcctcctgc tggcaggcct gtgctgcctg 2881 gtccctgtct ccctggctga ggatccccag ggagatgctg cccagaagac agatacatcc 2941 caccatgatc aggatcaccc aaccttcaac aagatcaccc ccaacctggc tgagttcgcc 3001 ttcagcctat accgccagct ggcacaccag tccaacagca ccaatatctt cttctcccca 3061 gtgagcatcg ctacagcctt tgcaatgctc tccctgggga ccaaggctga cactcacgat 3121 gaaatcctgg agggcctgaa tttcaacctc acggagattc cggaggctca gatccatgaa 3181 ggcttccagg aactcctccg taccctcaac cagccagaca gccagctcca gctgaccacc 3241 ggcaatggcc tgttcctcag cgagggcctg aagctagtgg ataagttttt ggaggatgtt 3301 aaaaagttgt accactcaga agccttcact gtcaacttcg gggacaccga agaggccaag 3361 aaacagatca acgattacgt ggagaagggt actcaaggga aaattgtgga tttggtcaag 3421 gagcttgaca gagacacagt ttttgctctg gtgaattaca tcttctttaa aggcaaatgg 3481 gagagaccct ttgaagtcaa ggacaccgag gaagaggact tccacgtgga ccaggtgacc 3541 accgtgaagg tgcctatgat gaagcgttta ggcatgttta acatccagca ctgtaagaag 3601 ctgtccagct gggtgctgct gatgaaatac ctgggcaatg ccaccgccat cttcttcctg 3661 cctgatgagg ggaaactaca gcacctggaa aatgaactca cccacgatat catcaccaag 3721 ttcctggaaa atgaagacag aaggtctgcc agcttacatt tacccaaact gtccattact 3781 ggaacctatg atctgaagag cgtcctgggt caactgggca tcactaaggt cttcagcaat 3841 ggggctgacc tctccggggt cacagaggag gcacccctga agctctccaa ggccgtgcat 3901 aaggctgtgc tgaccatcga cgagaaaggg actgaagctg ctggggccat gtttttagag 3961 gccataccca tgtctatccc ccccgaggtc aagttcaaca aaccctttgt cttcttaatg 4021 attgaacaaa ataccaagtc tcccctcttc atgggaaaag tggtgaatcc cacccaaaaa 4081 taagaattct gcagtcgacg gtaccgcggg cccgggatcc cgcgactcta gataattcta 4141 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct 4201 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag 4261 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt 4321 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc 4381 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct 4441 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag 4501 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc 4561 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc 4621 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc 4681 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 4741 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 4801 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 4861 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 4921 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 4981 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 5041 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 5101 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 5161 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 5221 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaccgcgtct ggaacaatca 5281 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 5341 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 5401 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 5461 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 5521 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 5581 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 5641 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg 5701 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 5761 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 5821 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc 5881 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct 5941 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag 6001 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 6061 aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg 6121 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc 6181 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta 6241 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg 6301 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac 6361 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag 6421 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga 6481 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga 6541 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 6601 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 6661 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 6721 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg 6781 acattgctag cgttttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 6841 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 6901 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 6961 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 7021 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 7081 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 7141 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 7201 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 7261 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 7321 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 7381 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 7441 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 7501 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 7561 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 7621 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 7681 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 7741 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 7801 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 7861 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 7921 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 7981 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 8041 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 8101 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 8161 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 8221 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 8281 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 8341 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 8401 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 8461 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 8521 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 8581 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 8641 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 8701 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 8761 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 8821 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 8881 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 8941 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 9001 gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 9061 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 9121 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt 9181 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt 9241 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac 9301 ttagtagttt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt //