LOCUS V038295 9577 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038295 VERSION V038295 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..4273 /label="MAZ(NM_002383)" /note="MAZ(NM_002383)" /gene="MAZ" CDS 4280..4345 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4365..4864 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 4885..5484 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5498..6086 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6293..6926 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7055..7071) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7079..7095 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7103..7133) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7148..7169 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7457..8045) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8216..9076) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9077..9181) /gene="bla" /label="AmpR promoter" polyA_signal 9229..9363 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgttccc ggtgtttcct tgcacgctgc tggccccccc 2881 cttccccgtg ctgggcctgg actcccgggg ggtgggcggc ctcatgaact ccttcccgcc 2941 acctcagggt cacgcccaga accccctgca ggtcggggct gagctccagt cccgcttctt 3001 tgcctcccag ggctgcgccc agagtccatt ccaggccgcg ccggcgcccc cgcccacgcc 3061 ccaggccccg gcggccgagc ccctccaggt ggacttgctc ccggtgctcg ccgccgccca 3121 ggagtccgcc gcggctgctg cggccgctgc cgccgctgct gccgccgtcg ctgccgcgcc 3181 cccggcccct gccgccgcct ctacggtgga cacagcggcc ctgaagcagc ctccggcgcc 3241 ccctccgcca cccccgccag tgtcggcgcc cgcggccgag gccgcgcccc ccgcctccgc 3301 cgccactatc gccgcggcgg cggccaccgc cgtcgtagcc ccaacctcga cggtcgccgt 3361 ggccccggtc gcgtctgcct tggagaagaa gacaaagagc aaggggccct acatctgcgc 3421 tctgtgcgcc aaggagttca agaacggcta caatctccgg aggcacgaag ccatccacac 3481 gggagccaag gccggccggg tcccctcggg tgctatgaag atgccgacca tggtgcccct 3541 gagcctcctg agcgtgcccc agctgagcgg agccggcggg ggagggggag aggcgggtgc 3601 cggcggcggc gctgccgcag tggccgccgg tggcgtggtg accacgaccg cctcggggaa 3661 gcgcatccgg aagaaccatg cctgcgagat gtgtggcaag gccttccgcg acgtctacca 3721 cctgaaccga cacaagctgt cgcactcgga cgagaagccc taccagtgcc cggtgtgcca 3781 gcagcgcttc aagcgcaagg accgcatgag ctaccacgtg cgctcacatg acggcgctgt 3841 gcacaagccc tacaactgct cccactgtgg caagagcttc tcccggccgg atcacctcaa 3901 cagtcacgtc agacaagtgc actcaacaga acggcccttc aaatgtgaga aatgtgaggc 3961 agctttcgcc acgaaggatc ggctgcgggc gcacacagta cgacacgagg agaaagtgcc 4021 atgtcacgtg tgtggcaaga tgctgagctc ggcttatatt tcggaccaca tgaaggtgca 4081 cagccagggt cctcaccatg tctgtgagct ctgcaacaaa ggtactggtg aggtttgtcc 4141 aatggcggcg gcagcggcag cggcggcagc ggcagcagcg gcagcagtag cagcccctcc 4201 cacagctgtg ggctccctct cgggggcgga gggggtgcct gtgagctctc agccacttcc 4261 ctcccaaccc tggggatccg actacaaaga ccatgacggt gattataaag atcatgacat 4321 cgactacaag gatgacgatg acaagtagtc tagataattc taccgggtag gggaggcgct 4381 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac 4441 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt 4501 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc 4561 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt 4621 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata 4681 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg 4741 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt 4801 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct 4861 ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc 4921 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 4981 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 5041 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 5101 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 5161 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 5221 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 5281 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 5341 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 5401 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 5461 atgacccgca agcccggtgc ctgaccgcgt ctggaacaat caacctctgg attacaaaat 5521 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 5581 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 5641 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 5701 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 5761 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 5821 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 5881 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct 5941 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 6001 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg 6061 gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa 6121 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa 6181 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg 6241 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg 6301 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc 6361 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt 6421 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga 6481 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa 6541 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat 6601 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac 6661 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata 6721 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga 6781 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 6841 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 6901 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 6961 ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgttttac 7021 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 7081 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 7141 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 7201 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 7261 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 7321 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 7381 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 7441 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 7501 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 7561 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 7621 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 7681 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 7741 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 7801 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 7861 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 7921 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 7981 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 8041 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 8101 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 8161 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 8221 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 8281 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 8341 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 8401 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 8461 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 8521 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 8581 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 8641 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 8701 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 8761 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 8821 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 8881 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 8941 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 9001 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 9061 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 9121 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 9181 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt 9241 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 9301 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 9361 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt 9421 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt 9481 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt ttttaaagaa 9541 attgtatttg ttaaatatgt actacaaact tagtagt //