LOCUS V037961 9142 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V037961 VERSION V037961 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..3838 /label="LDHA(NM_005566)" /note="LDHA(NM_005566)" /gene="LDHA" CDS 3845..3910 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 3930..4429 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 4450..5049 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5063..5651 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5858..6491 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6620..6636) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6644..6660 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6668..6698) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6713..6734 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7022..7610) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7781..8641) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8642..8746) /gene="bla" /label="AmpR promoter" polyA_signal 8794..8928 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatggcaac tctaaaggat cagctgattt ataatcttct 2881 aaaggaagaa cagacccccc agaataagat tacagttgtt ggggttggtg ctgttggcat 2941 ggcctgtgcc atcagtatct taatgaagga cttggcagat gaacttgctc ttgttgatgt 3001 catcgaagac aaattgaagg gagagatgat ggatctccaa catggcagcc ttttccttag 3061 aacaccaaag attgtctctg gcaaagacta taatgtaact gcaaactcca agctggtcat 3121 tatcacggct ggggcacgtc agcaagaggg agaaagccgt cttaatttgg tccagcgtaa 3181 cgtgaacatc tttaaattca tcattcctaa tgttgtaaaa tacagcccga actgcaagtt 3241 gcttattgtt tcaaatccag tggatatctt gacctacgtg gcttggaaga taagtggttt 3301 tcccaaaaac cgtgttattg gaagcggttg caatctggat tcagcccgat tccgttacct 3361 aatgggggaa aggctgggag ttcacccatt aagctgtcat gggtgggtcc ttggggaaca 3421 tggagattcc agtgtgcctg tatggagtgg aatgaatgtt gctggtgtct ctctgaagac 3481 tctgcaccca gatttaggga ctgataaaga taaggaacag tggaaagagg ttcacaagca 3541 ggtggttgag agtgcttatg aggtgatcaa actcaaaggc tacacatcct gggctattgg 3601 actctctgta gcagatttgg cagagagtat aatgaagaat cttaggcggg tgcacccagt 3661 ttccaccatg attaagggtc tttacggaat aaaggatgat gtcttcctta gtgttccttg 3721 cattttggga cagaatggaa tctcagacct tgtgaaggtg actctgactt ctgaggaaga 3781 ggcccgtttg aagaagagtg cagatacact ttgggggatc caaaaggagc tgcaatttgg 3841 atccgactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga 3901 cgatgacaag tagtctagat aattctaccg ggtaggggag gcgcttttcc caaggcagtc 3961 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc 4021 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt 4081 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc 4141 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac 4201 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt 4261 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct 4321 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa 4381 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc 4441 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc 4501 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc 4561 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 4621 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 4681 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 4741 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg 4801 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 4861 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 4921 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 4981 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 5041 ggtgcctgac cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac 5101 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 5161 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 5221 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 5281 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 5341 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 5401 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct 5461 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 5521 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 5581 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc 5641 cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca 5701 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag 5761 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct 5821 gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa 5881 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag 5941 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag 6001 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg 6061 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg 6121 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag 6181 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt 6241 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt 6301 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 6361 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 6421 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 6481 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 6541 atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg acctctagct 6601 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 6661 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 6721 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 6781 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 6841 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 6901 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 6961 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 7021 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 7081 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 7141 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 7201 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 7261 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 7321 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 7381 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 7441 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 7501 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 7561 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 7621 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 7681 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 7741 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 7801 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 7861 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 7921 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 7981 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 8041 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 8101 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 8161 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 8221 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 8281 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 8341 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 8401 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 8461 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 8521 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 8581 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 8641 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 8701 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 8761 aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt 8821 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 8881 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact 8941 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac 9001 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat 9061 tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt atttgttaaa 9121 tatgtactac aaacttagta gt //