LOCUS V019414 9867 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019414 VERSION V019414 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2827..4593 /label="IL4I1(NM_001258017)" /note="IL4I1(NM_001258017)" /gene="IL4I1" CDS 4600..4623 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" promoter 4655..5154 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5175..5774 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5788..6376 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6583..7216 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7345..7361) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7369..7385 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7393..7423) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7438..7459 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7747..8335) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8506..9366) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9367..9471) /gene="bla" /label="AmpR promoter" polyA_signal 9519..9653 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 gccaccatgc ccaacgatga cttctgtcct gggctaacca taaaggccat gggtgctgag 2881 agagcccccc agaggcagcc atgcaccctg cacctcctcg tcctcgtccc catcctcctc 2941 agcctggtgg cctcccagga ctggaaggct gaacgcagcc aagacccctt cgagaaatgc 3001 atgcaggatc ctgactatga gcagctgctc aaggtggtga cctgggggct caatcggacc 3061 ctgaagcccc agagggtgat tgtggttggc gctggtgtgg ccgggctggt ggccgccaag 3121 gtgctcagcg atgctggaca caaggtcacc atcctggagg cagataacag gatcgggggc 3181 cgcatcttca cctaccggga ccagaacacg ggctggattg gggagctggg agccatgcgc 3241 atgcccagct ctcacaggat cctccacaag ctctgccagg gcctggggct caacctgacc 3301 aagttcaccc agtacgacaa gaacacgtgg acggaggtgc acgaagtgaa gctgcgcaac 3361 tatgtggtgg agaaggtgcc cgagaagctg ggctacgcct tgcgtcccca ggaaaagggc 3421 cactcgcccg aagacatcta ccagatggct ctcaaccagg ccctcaaaga cctcaaggca 3481 ctgggctgca gaaaggcgat gaagaagttt gaaaggcaca cgctcttgga atatcttctc 3541 ggggagggga acctgagccg gccggccgtg cagcttctgg gagacgtgat gtccgaggat 3601 ggcttcttct atctcagctt cgccgaggcc ctccgggccc acagctgcct cagcgacaga 3661 ctccagtaca gccgcatcgt gggtggctgg gacctgctgc cgcgcgcgct gctgagctcg 3721 ctgtccgggc ttgtgctgtt gaacgcgccc gtggtggcga tgacccaggg accgcacgat 3781 gtgcacgtgc agatcgagac ctctcccccg gcgcggaatc tgaaggtgct gaaggccgac 3841 gtggtgctgc tgacggcgag cggaccggcg gtgaagcgca tcaccttctc gccgccgctg 3901 ccccgccaca tgcaggaggc gctgcggagg ctgcactacg tgccggccac caaggtgttc 3961 ctaagcttcc gcaggccctt ctggcgcgag gagcacattg aaggcggcca ctcaaacacc 4021 gatcgcccgt cgcgcatgat tttctacccg ccgccgcgcg agggcgcgct gctgctggcc 4081 tcgtacacgt ggtcggacgc ggcggcagcg ttcgccggct tgagccggga agaggcgttg 4141 cgcttggcgc tcgacgacgt ggcggcattg cacgggcctg tcgtgcgcca gctctgggac 4201 ggcaccggcg tcgtcaagcg ttgggcggag gaccagcaca gccagggtgg ctttgtggta 4261 cagccgccgg cgctctggca aaccgaaaag gatgactgga cggtccctta tggccgcatc 4321 tactttgccg gcgagcacac cgcctacccg cacggctggg tggagacggc ggtcaagtcg 4381 gcgctgcgcg ccgccatcaa gatcaacagc cggaaggggc ctgcatcgga cacggccagc 4441 cccgaggggc acgcatctga catggagggg caggggcatg tgcatggggt ggccagcagc 4501 ccctcgcatg acctggcaaa ggaagaaggc agccaccctc cagtccaagg ccagttatct 4561 ctccaaaaca cgacccacac gaggacctcg catgaattcg attacaagga tgacgacgat 4621 aagtgaggat cccgcgactc tagataattc taccgggtag gggaggcgct tttcccaagg 4681 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc 4741 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc 4801 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc 4861 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac 4921 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc 4981 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc 5041 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt 5101 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc 5161 agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg 5221 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca 5281 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc 5341 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct 5401 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg 5461 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc 5521 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg 5581 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg 5641 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct 5701 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca 5761 agcccggtgc ctgaccgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga 5821 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 5881 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 5941 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 6001 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 6061 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 6121 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 6181 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 6241 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 6301 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 6361 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa 6421 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg 6481 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg 6541 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact 6601 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg 6661 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct 6721 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca 6781 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt 6841 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact 6901 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga 6961 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg 7021 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 7081 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 7141 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 7201 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca 7261 aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgttttac cgtcgacctc 7321 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 7381 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 7441 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 7501 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 7561 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 7621 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 7681 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 7741 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 7801 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 7861 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 7921 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 7981 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 8041 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 8101 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 8161 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 8221 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 8281 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 8341 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 8401 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 8461 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 8521 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 8581 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 8641 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 8701 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 8761 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 8821 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 8881 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 8941 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 9001 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 9061 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 9121 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 9181 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 9241 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 9301 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 9361 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 9421 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 9481 ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata 9541 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 9601 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga 9661 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca 9721 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa 9781 gaaattgtat ttgttaaata tgtactacaa acttagtagt ttttaaagaa attgtatttg 9841 ttaaatatgt actacaaact tagtagt //