LOCUS V044367 10561 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V044367 VERSION V044367 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..5257 /label="Ahr(NM_013464)" /note="Ahr(NM_013464)" /gene="Ahr" CDS 5264..5329 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 5349..5848 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5869..6468 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6482..7070 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7277..7910 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(8039..8055) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8063..8079 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8087..8117) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8132..8153 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8441..9029) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9200..10060) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10061..10165) /gene="bla" /label="AmpR promoter" polyA_signal 10213..10347 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgagcag cggcgccaac atcacctatg ccagccgcaa 2881 gcggcgcaag ccggtgcaga aaacagtaaa gcccatcccc gctgaaggaa ttaagtcaaa 2941 tccttctaag cgacacagag accggctgaa cacagagtta gaccgcctgg ccagcctgct 3001 gcccttcccg caagatgtta ttaataagct ggacaaactc tctgttctta ggctcagcgt 3061 cagctacctg agggccaaga gcttctttga tgttgcatta aagtccaccc ctgctgacag 3121 aaatggaggc caggaccagt gtagagcaca aatcagagac tggcaggatt tgcaagaagg 3181 agagttcttg ttacaggcgc tgaatggctt tgtgctggtt gtcacagcag atgccttggt 3241 cttctatgct tcctccacta tccaagatta cctgggcttt cagcagtctg atgtcatcca 3301 tcagagcgta tatgagctca tccatacaga agaccgggcg gaattccagc gccagcttca 3361 ctgggctcta aacccagact ctgcacaagg agtggacgaa gcccatggcc ctccacaggc 3421 agcagtctat tataccccag accagcttcc tccagagaac gcttctttca tggagaggtg 3481 cttcaggtgc cggctgaggt gcctgctgga taattcatct ggttttctgg caatgaattt 3541 ccaagggagg ttaaagtatc ttcatggaca gaacaagaaa gggaaggacg gagcgctgct 3601 tcctccacaa ctggctttgt ttgcaatagc tactccactt cagccaccct ccatcctgga 3661 aattcgaacc aaaaacttca tcttcaggac caaacacaag ctagacttca cacctattgg 3721 ttgtgatgcc aaagggcagc ttattctggg ctatacagaa gtagagctgt gcacaagagg 3781 atcggggtac cagttcatcc atgctgcaga catacttcac tgtgcagaat cccacatccg 3841 catgattaag actggagaaa gtggcatgac agttttccgg cttcttgcaa aacacagtcg 3901 ctggaggtgg gtccagtcca atgcacgctt gatttacaga aatggaagac cagattacat 3961 catcgccact cagagaccac tgacggatga agaaggacga gagcatttac agaagcgaag 4021 tacgtcgctg cccttcatgt ttgctaccgg agaggctgtg ttgtacgaga tctccagccc 4081 tttctctccc ataatggatc ccctaccaat acgcaccaaa agcaacacta gcaggaaaga 4141 ctgggctccc cagtcaaccc caagtaagga ttctttccac cccagttctc ttatgagtgc 4201 cctcatccag caggatgagt ccatctatct gtgtcctcct tcaagccctg cgctgttaga 4261 cagccatttt ctcatgggct ccgtgagcaa gtgcgggagt tggcaagaca gctttgcggc 4321 cgcaggaagt gaggctgcgc tgaaacatga gcaaattggc catgctcagg acgtgaacct 4381 tgcactctct ggcggcccct cagagctctt tccggataat aaaaataatg acttgtacag 4441 catcatgagg aaccttggga ttgattttga agatatcaga agcatgcaga acgaggagtt 4501 cttcagaact gactccaccg ctgctggtga ggttgacttc aaagacatcg acataacgga 4561 cgaaatcctg acctacgtgc aggattccct gaacaattca actttgctga actcggcttg 4621 ccagcagcag cctgtgactc agcacctaag ctgtatgctg caggagcgcc tgcaactaga 4681 gcaacagcaa cagcttcagc agcccccgcc gcaggctctg gagccccagc agcagctgtg 4741 tcagatggtg tgcccccagc aagatctggg tccgaagcac acgcaaatca acggcacgtt 4801 tgcaagttgg aaccccaccc ctcccgtgtc tttcaactgt ccccagcagg aactaaagca 4861 ctatcagctc ttttccagct tacaggggac tgctcaggaa tttccctaca aaccagaggt 4921 ggacagtgtg ccttacacac agaactttgc tccctgtaat cagcctctgc ttccagaaca 4981 ttccaagagt gtgcagttgg acttccctgg aagggatttt gaaccgtccc tgcatcccac 5041 tacttctaat ttagattttg tcagttgttt acaagttcct gaaaaccaaa gtcatgggat 5101 aaactcacag tccgccatgg tcagtcctca ggcatactat gctggggcca tgtccatgta 5161 tcagtgccag ccagggccac agcgcacccc tgtggaccag acgcagtaca gctctgaaat 5221 tccaggttct caggcattcc taagcaaggt gcagagtgga tccgactaca aagaccatga 5281 cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt aatctagata 5341 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 5401 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 5461 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 5521 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 5581 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 5641 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 5701 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 5761 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 5821 ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac 5881 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 5941 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 6001 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 6061 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 6121 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 6181 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 6241 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 6301 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 6361 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 6421 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa 6481 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 6541 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 6601 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 6661 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 6721 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 6781 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 6841 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 6901 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 6961 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 7021 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa 7081 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc 7141 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca 7201 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 7261 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat 7321 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca 7381 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat 7441 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat 7501 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt 7561 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc 7621 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag 7681 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg 7741 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 7801 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 7861 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 7921 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 7981 gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg taatcatggt 8041 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 8101 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 8161 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 8221 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 8281 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 8341 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 8401 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 8461 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 8521 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 8581 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 8641 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 8701 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 8761 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 8821 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 8881 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 8941 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 9001 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 9061 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 9121 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 9181 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 9241 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 9301 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 9361 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 9421 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 9481 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 9541 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 9601 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 9661 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 9721 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 9781 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 9841 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 9901 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 9961 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 10021 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 10081 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 10141 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg 10201 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 10261 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 10321 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc 10381 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa 10441 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact 10501 acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca aacttagtag 10561 t //