LOCUS V044592 10264 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V044592 VERSION V044592 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..4939 /label="Acsl1(NM_001424792)" /note="Acsl1(NM_001424792)" /gene="Acsl1" CDS 4946..4972 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4976..5002 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 5006..5032 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 5052..5551 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5572..6171 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6185..6773 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6980..7613 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7742..7758) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7766..7782 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7790..7820) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7835..7856 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8144..8732) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8903..9763) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9764..9868) /gene="bla" /label="AmpR promoter" polyA_signal 9916..10050 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatggaagt ccatgaattg ttccggtatt ttcgaatgcc 2881 agagctgatt gacattcggc agtacgtgcg cacccttcca accaacaccc tcatggggtt 2941 tggggctttt gcagcgctca ccaccttctg gtatgccacc aggcctaagg ccctgaagcc 3001 accatgtgac ctctccatgc agtcagtgga aatagcgggt accactgatg gtattcgaag 3061 atcagcagtc cttgaagatg acaagctctt ggtgtactac tacgacgatg tcagaaccat 3121 gtacgatggc ttccagaggg ggattcaggt gtcaaataat ggtccttgtt taggttctcg 3181 gaagccaaac cagccctatg agtggatttc ctacaaagag gtggcagaac tggctgagtg 3241 cataggctcc gggctgatcc agaaggggtt caagccttgc tccgagcagt tcatcggcct 3301 cttctctcaa aacagacccg agtgggtgat cgtcgagcaa ggatgcttct cttactcaat 3361 ggtggtcgtc ccgctctatg acacccttgg agctgacgcc atcacctaca tagtgaacaa 3421 agctgaactc tctgtgattt ttgctgacaa gccagaaaaa gccaaactct tattagaagg 3481 tgtagaaaac aagttaacac catgccttaa aatcatagtc atcatggact cctacggcag 3541 tgatctggtg gaacgaggca agaagtgtgg ggtggaaatc atcagcctca aagctctgga 3601 ggaccttgga agagtgaaca gagtgaagcc caagcctcca gaacccgaag atcttgcgat 3661 aatttgtttc acaagtggaa ctacaggcaa ccccaaagga gcaatgatca ctcaccaaaa 3721 cattataaac gactgctcag gttttataaa agcaacagag agtgcattca tcgcttccac 3781 agatgatgtg ctgatatctt tcttgcctct cgcccatatg tttgagaccg ttgtagagtg 3841 tgtaatgctg tgtcatggag ctaagatagg atttttccaa ggagatatca ggctgcttat 3901 ggacgacctc aaggtgcttc agcccaccat cttccctgtg gttcccaggc tgctgaaccg 3961 gatgttcgac agaatttttg gacaagcaaa cacttccttg aagcgatggc tgttggactt 4021 tgcctccaaa aggaaagagg cggagcttcg cagtggcatc gtcagaaaca acagcctgtg 4081 ggataaactc atcttccaca agatacagtc gagcctgggt gggaaagtcc ggctgatgat 4141 cacaggagca gccccggtgt ctgccacagt gctgacgttt ctgaggacag cgctcggctg 4201 ccagttctat gaaggctacg gacagaccga gtgcactgct ggttgctgcc tgagcttgcc 4261 cggagactgg acggcaggcc atgttggagc ccccatgcct tgcaattatg taaagcttgt 4321 ggatgtggaa gaaatgaatt acctggcatc caagggcgag ggtgaggtgt gtgtgaaagg 4381 ggcaaatgtg ttcaaaggct acttgaaaga cccagcaaga acagctgaag ccctggataa 4441 agatggctgg ttacacacgg gggacattgg aaaatggctg ccaaatggca ccttgaagat 4501 tatcgacagg aaaaagcaca tatttaaact agcccaagga gagtacatag caccagaaaa 4561 gattgaaaat atctacctgc ggagtgaagc cgtggcccag gtgtttgtcc acggagaaag 4621 cttgcaggcc tttctcatag cagttgtggt acccgacgtt gagagcctac cgtcctgggc 4681 acagaagaga ggcttacaag ggtccttcga agaactgtgc aggaacaagg atatcaataa 4741 agctatcctg gacgacttgt tgaaacttgg gaaggaagcc ggtctgaagc catttgaaca 4801 ggtcaaaggc attgctgtgc acccggaatt attttctatt gacaacggcc ttctgactcc 4861 aacactgaag gcgaagaggc cagagctacg gaactatttc aggtcgcaga tagatgaact 4921 gtacgccacc atcaagatcg gatcctaccc atacgacgta cctgattatg caacttatcc 4981 ttatgatgta cctgattatg ctagctatcc ttatgatgtc ccagactacg catagtctag 5041 ataattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc 5101 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc 5161 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc 5221 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat 5281 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg 5341 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag 5401 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc 5461 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct 5521 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag 5581 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 5641 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 5701 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 5761 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 5821 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 5881 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 5941 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 6001 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 6061 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 6121 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg 6181 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 6241 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 6301 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 6361 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 6421 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 6481 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 6541 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 6601 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 6661 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 6721 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat 6781 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct 6841 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc 6901 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 6961 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt 7021 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg 7081 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca 7141 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg 7201 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca 7261 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct 7321 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg 7381 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct 7441 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 7501 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 7561 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 7621 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 7681 gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg gcgtaatcat 7741 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 7801 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 7861 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 7921 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 7981 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 8041 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 8101 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 8161 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 8221 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 8281 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 8341 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 8401 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 8461 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 8521 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 8581 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 8641 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 8701 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 8761 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 8821 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 8881 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 8941 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 9001 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 9061 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 9121 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 9181 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 9241 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 9301 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 9361 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 9421 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 9481 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 9541 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 9601 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 9661 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 9721 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 9781 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 9841 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg 9901 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc 9961 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 10021 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat 10081 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc 10141 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt 10201 actacaaact tagtagtttt taaagaaatt gtatttgtta aatatgtact acaaacttag 10261 tagt //