LOCUS       V032190                11025 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032190
VERSION     V032190
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2815..5562
                     /label="OSBP2(NM_030758)"
                     /note="OSBP2(NM_030758)"
                     /gene="OSBP2"
     misc_feature    5599..6185
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             6186..6980
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6994..7582
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7789..8422
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8550..8566)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8574..8590
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8598..8628)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8643..8664
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8952..9540)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9711..10571)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10572..10676)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10724..10858
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgggg
     2821 aaagcggcgg ctccgagccg aggcggcggc tgtggcggcc gctcccgcgg gctctcgtcg
     2881 ctgttcacgg ttgtcccctg cctgtcgtgc cacacggcgg cgccgggcat gagcgcttcc
     2941 acgtccggct ccgggccgga gcccaagccc cagccccagc ccgtgcccga accggagcgg
     3001 ggaccgctgt cagaacaggt gtcggaggca gtttcggagg cagtgccaag atcggaacct
     3061 gtgtccgaga cgacgtctga gccggagcca ggggctgggc agccatcgga actgctgcag
     3121 gggtcgcggc cggggtcaga gtcaagctca ggtgtagggg ctgggccctt cactaaggcc
     3181 gcatcggagc cgctctcccg ggcggtgggg agcgcgacct ttctcagacc cgagtcagga
     3241 tcgctgccag cgttaaagcc cctgcctctt ctgcgaccag gacaggcgaa gactcctctt
     3301 ggggttccaa tgtcggggac tggcacgacc tccagtgccc cactggcctt actgcctctg
     3361 gacagcttcg agggctggct tctcaagtgg accaactatc tgaagggcta ccagcgccgc
     3421 tggttcgtgc tgggcaatgg tttgctctct tactacagaa atcagggtga aatggcccac
     3481 acgtgccgtg gaaccatcaa cctgtccacc gcgcacattg acacggagga ctcttgtggt
     3541 atcttgctga ccagtggggc caggagctac cacctcaagg ccagctcaga ggtggaccgg
     3601 cagcagtgga tcaccgccct ggagctggcc aaggccaagg ctgtccgcgt gatgaacact
     3661 cattcagatg actctgggga cgacgacgag gctaccaccc cagccgacaa gagcgagctg
     3721 caccacaccc tgaagaatct ttccctgaag ttagatgacc tcagcacgtg caatgacctc
     3781 atcgccaagc acggcgctgc actccagcgc tccctgacag agctggacgg cctcaagatc
     3841 ccatctgaga gtggggagaa gctgaaggtg gtgaatgagc gggccaccct cttccgcatc
     3901 acatccaatg ctatgatcaa cgcctgcagg gacttcttgg aactagcaga gatacacagt
     3961 cggaaatggc agcgggcact gcagtatgag caggagcagc gcgtgcactt ggaggaaacc
     4021 attgagcagc tggcgaagca gcacaacagc ctcgagcggg ccttccacag tgcccctggc
     4081 cggccggcca acccctccaa gagcttcatt gagggaagcc tcttgactcc caaaggagag
     4141 gacagtgagg aagatgaaga taccgagtac tttgatgcca tggaagactc cacatccttc
     4201 atcaccgtga tcaccgaggc caaggaagac agcagaaaag ctgaaggtag caccgggaca
     4261 agttccgtgg actggagctc agcagacaat gtactagatg gtgcctcgct cgtgcccaag
     4321 ggttcatcca aagtcaagag gcgagtccgc attcccaaca agcccaacta cagccttaac
     4381 ctctggagca tcatgaagaa ctgcatcggc cgggagctct ccaggatccc catgccggtg
     4441 aacttcaatg agcccctgtc catgctccag cggctgacag aggacctgga gtaccaccac
     4501 ctgctggaca aggcagtgca ctgcaccagc tcagtggagc agatgtgcct ggtggccgcc
     4561 ttctctgtgt cctcctactc caccacagtg caccgcatcg ccaagccctt caaccccatg
     4621 ctgggggaga ccttcgagct ggaccgcctc gacgacatgg gcctgcgctc cctctgtgag
     4681 caggtgagcc accacccccc ctcagctgcg cactacgtgt tctccaagca tggctggagc
     4741 ctctggcagg agatcaccat ctccagcaag ttccggggaa aatacatctc catcatgccg
     4801 ctaggtgcca tccacttaga attccaggcc agtgggaatc actacgtgtg gaggaagagc
     4861 acctcaactg ttcacaacat catcgtgggc aagctctgga tcgaccagtc aggggacatc
     4921 gagattgtga accataagac caatgaccgg tgccagctga agttcctgcc ctacagctac
     4981 ttctccaaag aggcagcccg gaaggtgaca ggagtggtga gtgacagcca gggcaaggcc
     5041 cattacgtgc tgtccggctc gtgggatgaa caaatggagt gctccaaggt catgcatagc
     5101 agtcccagca gccccagctc tgacgggaag cagaagacag tgtaccagac cctgtcagcc
     5161 aagctgctgt ggaagaagta cccgctgccg gagaacgcgg agaacatgta ctacttctca
     5221 gagctggccc tgaccctcaa cgagcacgag gagggcgtag cgccaaccga cagccgcctg
     5281 cggcccgacc agcggctgat ggagaagggc cgttgggacg aggccaatac cgagaagcag
     5341 cggctggagg agaagcagcg cctgtcgcgg cgccggcggc tggaggcctg cgggccgggc
     5401 agcagctgca gctcggagga agagaaggag gcggatgcct acacgccact gtggtttgag
     5461 aagaggctgg atccgctgac cggggagatg gcctgtgtgt acaagggcgg ctactgggag
     5521 gccaaggaga agcaagactg gcatatgtgc cccaacatct tctagctcga gactagttct
     5581 agagcggccg cggatccgcc cctctccctc ccccccccct aacgttactg gccgaagccg
     5641 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     5701 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     5761 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     5821 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     5881 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     5941 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     6001 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     6061 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     6121 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttgcca
     6181 caaccatggc tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     6241 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     6301 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     6361 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     6421 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     6481 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     6541 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     6601 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     6661 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     6721 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     6781 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     6841 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     6901 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     6961 ttcttgacga gttcttctga acgcgtctgg aacaatcaac ctctggatta caaaatttgt
     7021 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     7081 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     7141 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     7201 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     7261 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7321 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7381 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     7441 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7501 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7561 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg
     7621 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     7681 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     7741 acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa
     7801 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     7861 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     7921 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     7981 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
     8041 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
     8101 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
     8161 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     8221 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     8281 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8341 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8401 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     8461 cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg tttaccgtcg
     8521 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8581 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8641 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8701 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8761 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8821 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8881 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8941 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9001 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9061 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9121 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9181 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9241 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9301 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9361 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9421 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9481 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9541 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9601 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9661 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9721 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9781 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9841 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9901 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9961 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    10021 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    10081 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    10141 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10201 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10261 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10321 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10381 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10441 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10501 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10561 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10621 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10681 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc
    10741 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    10801 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    10861 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    10921 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
    10981 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
