LOCUS pLVX-EF1-alpha-L 10441 bp DNA circular SYN 08-APR-2021 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS pLVX-EF1-alpha-Luc-N1. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10441) AUTHORS caoheibi TITLE Direct Submission REFERENCE 2 (bases 1 to 10441) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..10441 /mol_type="other DNA" /organism="synthetic DNA construct" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /label=gp41 peptide /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 1914..1955 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" misc_feature 2027..2144 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2338..3519 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" misc_feature 3534..3599 /label=MCS /note="multiple cloning site" CDS 3606..5255 /label=luciferase /note="firefly luciferase" promoter 5276..5775 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" CDS 5796..6392 /label=PuroR /note="puromycin N-acetyltransferase" misc_feature 6409..6997 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" LTR 7204..7837 /label=5' LTR /note="5' long terminal repeat (LTR) from HIV-1" primer_bind complement(7966..7982) /label=M13 rev /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 7990..8006 /label=lac repressor encoded by lacI binding site /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8014..8044) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(8059..8080) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(8368..8956) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(9130..9987) /label=AmpR /note="beta-lactamase" promoter complement(9988..10092) /label=AmpR promoter polyA_signal 10140..10274 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatcgctagc gctaccggac 3541 tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatccg 3601 ccaccatgga agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag 3661 atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa 3721 caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa 3781 tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg 3841 tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg 3901 gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg 3961 gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg 4021 tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc 4081 agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat 4141 acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct 4201 ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat 4261 tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa 4321 gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg 4381 gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg 4441 attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca 4501 ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc 4561 tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag 4621 gatatgggct cactgagact acatcagcta ttctgattac acccgagggg gatgataaac 4681 cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg 4741 ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt 4801 ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac 4861 attctggaga catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga 4921 agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc 4981 tccaacaccc caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg 5041 aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg 5101 tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg 5161 tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc 5221 tcataaaggc caagaagggc ggaaagatcg ccgtgtaatt ctagataatt ctaccgggta 5281 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact 5341 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa 5401 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag 5461 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc 5521 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc 5581 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg 5641 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga 5701 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca 5761 tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc 5821 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg 5881 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc 5941 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg 6001 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg 6061 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga 6121 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg 6181 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg 6241 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc 6301 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc 6361 gcacctggtg catgacccgc aagcccggtg cctgaccgcg tctggaacaa tcaacctctg 6421 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 6481 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 6541 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 6601 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 6661 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 6721 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 6781 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 6841 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 6901 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct 6961 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag 7021 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg 7081 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt 7141 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg 7201 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc 7261 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc 7321 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg 7381 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc 7441 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc 7501 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt 7561 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca 7621 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc 7681 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 7741 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 7801 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 7861 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc 7921 tagcgtttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 7981 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 8041 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 8101 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 8161 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 8221 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 8281 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 8341 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 8401 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 8461 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 8521 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 8581 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 8641 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 8701 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 8761 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 8821 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 8881 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 8941 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 9001 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 9061 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 9121 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 9181 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 9241 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 9301 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 9361 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 9421 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 9481 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 9541 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 9601 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 9661 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 9721 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 9781 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 9841 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 9901 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 9961 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 10021 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 10081 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca 10141 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 10201 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 10261 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc 10321 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct 10381 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag 10441 t //