LOCUS pLVX-EF1-alpha-A 9519 bp DNA circular SYN 08-APR-2021 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS pLVX-EF1-alpha-AcGFP1-C1. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9519) AUTHORS caoheibi TITLE Direct Submission REFERENCE 2 (bases 1 to 9519) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..9519 /mol_type="other DNA" /organism="synthetic DNA construct" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /label=gp41 peptide /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 1914..1955 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" misc_feature 2027..2144 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2338..3519 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" CDS 3539..4255 /label=AcGFP1 /note="Aequorea coerulescens GFP" misc_feature 4256..4321 /label=MCS /note="multiple cloning site" promoter 4354..4853 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" CDS 4874..5470 /label=PuroR /note="puromycin N-acetyltransferase" misc_feature 5487..6075 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" LTR 6282..6915 /label=5' LTR /note="5' long terminal repeat (LTR) from HIV-1" primer_bind complement(7044..7060) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(7068..7084) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7092..7122) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(7137..7158) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(7446..8034) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8208..9065) /label=AmpR /note="beta-lactamase" promoter complement(9066..9170) /label=AmpR promoter polyA_signal 9218..9352 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctaccgg tcgccaccat 3541 ggtgagcaag ggcgccgagc tgttcaccgg catcgtgccc atcctgatcg agctgaatgg 3601 cgatgtgaat ggccacaagt tcagcgtgag cggcgagggc gagggcgatg ccacctacgg 3661 caagctgacc ctgaagttca tctgcaccac cggcaagctg cctgtgccct ggcccaccct 3721 ggtgaccacc ctgagctacg gcgtgcagtg cttctcacgc taccccgatc acatgaagca 3781 gcacgacttc ttcaagagcg ccatgcctga gggctacatc caggagcgca ccatcttctt 3841 cgaggatgac ggcaactaca agtcgcgcgc cgaggtgaag ttcgagggcg ataccctggt 3901 gaatcgcatc gagctgaccg gcaccgattt caaggaggat ggcaacatcc tgggcaataa 3961 gatggagtac aactacaacg cccacaatgt gtacatcatg accgacaagg ccaagaatgg 4021 catcaaggtg aacttcaaga tccgccacaa catcgaggat ggcagcgtgc agctggccga 4081 ccactaccag cagaataccc ccatcggcga tggccctgtg ctgctgcccg ataaccacta 4141 cctgtccacc cagagcgccc tgtccaagga ccccaacgag aagcgcgatc acatgatcta 4201 cttcggcttc gtgaccgccg ccgccatcac ccacggcatg gatgagctgt acaagtccgg 4261 actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc 4321 caccggatct agataactga tcataattct accgggtagg ggaggcgctt ttcccaaggc 4381 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct 4441 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc 4501 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg 4561 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca 4621 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct 4681 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg 4741 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc 4801 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca 4861 gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt 4921 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac 4981 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg 5041 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg 5101 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc 5161 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca 5221 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg 5281 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt 5341 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt 5401 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa 5461 gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat 5521 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 5581 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 5641 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 5701 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 5761 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 5821 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 5881 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 5941 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 6001 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 6061 gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat 6121 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga 6181 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc 6241 agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc 6301 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga 6361 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta 6421 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag 6481 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg 6541 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt 6601 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag 6661 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc 6721 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct 6781 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt 6841 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt 6901 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa 6961 agaaatgaat atcagagagt gagaggcctt gacattgcta gcgttttacc gtcgacctct 7021 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 7081 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 7141 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 7201 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 7261 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 7321 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 7381 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 7441 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 7501 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 7561 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 7621 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 7681 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 7741 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 7801 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 7861 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 7921 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 7981 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 8041 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 8101 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 8161 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 8221 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 8281 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 8341 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 8401 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 8461 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 8521 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 8581 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 8641 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 8701 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 8761 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 8821 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 8881 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 8941 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 9001 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 9061 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 9121 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 9181 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa 9241 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 9301 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat 9361 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa 9421 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag 9481 aaattgtatt tgttaaatat gtactacaaa cttagtagt //