LOCUS       V012792                11085 bp    DNA     circular SYN 13-DEC-2021
DEFINITION  Exported.
ACCESSION   V012792
VERSION     V012792
KEYWORDS    pLVTHM
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11085)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 11085)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1300..1533
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1718..1762
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1911..1952
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        2122..3299
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     misc_feature    3347..3464
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             3554..4270
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    4343..4931
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     protein_bind    5099..5369
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     promoter        5398..5612
                     /label="H1 promoter"
                     /note="human H1 RNA promoter"
     protein_bind    complement(5647..5680)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5700..5880
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5905..5923)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        6711..6815
                     /label="AmpR promoter"
     CDS             6816..7673
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7847..8435
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        8681..9010
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     intron          10144..10209
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10339..10359
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    10784..10918
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgcgatgtcg acgataagct
     2041 ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag
     2101 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc
     2161 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg
     2221 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     2281 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     2341 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat
     2401 ggcccttgcg tgccttgaat tacttccact ggctgcagta cgtgattctt gatcccgagc
     2461 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
     2521 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     2581 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     2641 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
     2701 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     2761 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
     2821 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     2881 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     2941 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     3001 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     3061 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
     3121 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     3181 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     3241 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag
     3301 gaatttcgac atttaaattt aattaatctc gacggtatcg gttaactttt aaaagaaaag
     3361 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     3421 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttccgatc acgagactag
     3481 cctcgaggtt taaactacgg gatcttcgaa ggcctaagct tacgcgcgcg tcctagcgct
     3541 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     3601 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     3661 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     3721 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     3781 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     3841 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     3901 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     3961 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     4021 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     4081 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     4141 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     4201 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     4261 gctgtacaag tccggactca gatctcgata gctagtagct agctagctag tcgagctcaa
     4321 cttcggggac tagtcatatg ataatcaacc tctggattac aaaatttgtg aaagattgac
     4381 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     4441 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     4501 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     4561 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     4621 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     4681 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     4741 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     4801 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     4861 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     4921 cgcctccccg catcggtacg tatggccagg tacctttaag accaatgact tacaaggcag
     4981 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     5041 aacgaagaca agatgggatc aattcaccat gctagtggat ccccctcgag tttaccactc
     5101 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     5161 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     5221 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     5281 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     5341 agtttaccac tccctatcag tgatagagaa aagtgaaagt cggggctgca ggaattcgaa
     5401 cgctgacgtc atcaacccgc tccaaggaat cgcgggccca gtgtcactag gcgggaacac
     5461 ccagcgcgcg tgcgccctgg caggaagatg gctgtgaggg acaggggagt ggcgccctgc
     5521 aatatttgca tgtcgctatg tgttctggga aatcaccata aacgtgaaat gtctttggat
     5581 ttgggaatct tataagttct gtatgagacc acgcgtctcc aagcttatcg ataccgtcgc
     5641 atgggaataa cttcgtatag catacattat acgaagttat gctgcttttt gcttgtactg
     5701 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5761 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5821 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5881 gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca
     5941 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt
     6001 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg
     6061 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag
     6121 cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg
     6181 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
     6241 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
     6301 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
     6361 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
     6421 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     6481 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     6541 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     6601 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     6661 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6721 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6781 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6841 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6901 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6961 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     7021 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     7081 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     7141 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7201 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7261 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7321 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     7381 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7441 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7501 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7561 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7621 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7681 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7741 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7801 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7861 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7921 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7981 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8041 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8101 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8161 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8221 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8281 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8341 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8401 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8461 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8521 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8581 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8641 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
     8701 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8761 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8821 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     8881 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8941 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9001 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt
     9061 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
     9121 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
     9181 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     9241 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     9301 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg
     9361 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
     9421 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
     9481 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
     9541 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
     9601 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
     9661 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
     9721 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
     9781 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
     9841 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
     9901 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg
     9961 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
    10021 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag
    10081 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
    10141 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
    10201 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
    10261 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
    10321 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
    10381 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
    10441 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
    10501 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
    10561 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
    10621 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
    10681 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
    10741 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
    10801 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    10861 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    10921 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    10981 tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
    11041 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtag
//
