LOCUS       V046976                 9921 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046976
VERSION     V046976
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4003
                     /label="Slc30a9(NM_178651)"
                     /note="Slc30a9(NM_178651)"
                     /gene="Slc30a9"
     CDS             4010..4075
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4119..4330
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4343..4611
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             4646..5353
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5363..5416
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5417..6016
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6017..6604
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6678..6911
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6983..7104
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7123..7258
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7296..7312)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7320..7336
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7344..7374)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7389..7410
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7698..8286)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8457..9317)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9318..9422)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9896..9912
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgtttcc gggcttggcc gccgccgccg cggcgcacag
     2341 gtgcagctgg gcggcgctgt gcaggctcgg tggcggccgg gcagcgaccc gcggccgcag
     2401 ccagggatgg aagaatgtaa tgacatttga aagctttaca tatgtggttc ccgacattca
     2461 cccacattta tctattataa atcaagtcaa gttgtactct acgaatgtcc agaaaggagg
     2521 gcagggctca cagactccaa aagctgacaa agtcccatca cttacacaaa cagtagaaaa
     2581 tataggcgca gaactcaaag ctccacttaa gcaagaccct ctccaagtaa gagtcaaagc
     2641 agtccttaag aaaagggatt atggatcaaa atacactaag aataatttca tcactggagt
     2701 gagagcaata aacgagttct gccttaagtc cagtgattta gaacaacttc gaaaaatcag
     2761 gcggcgaagt ccccacgatg acaccgagtc ctttactgtg ttcttgaggt cagatgtgga
     2821 agcaaaagct ttggaagttt ggggaagcct tgaagctctt gccagagaga aaaaattgcg
     2881 taaagaagca gaaatagaat acagagaacg gctatttaga aaccaaagaa tattaagaga
     2941 atatggtgat ttcttgggaa acacaaagcc acgttccaga gcggtctcag tgttccttaa
     3001 gggaccagga aaagtggtca tggttgctat ttgcattaat ggcttaaact gcttctttaa
     3061 atttcttgcc tggatttata cgggttcagc aagtatgttc tcagaagcta tacactcatt
     3121 atctgatact tgtaaccagg gcttgctagc actgggcatc agcaaatctg ttcaaacacc
     3181 agatccttct cacccgtacg gcttttcaaa tatgcgctat atttcttcat tgattagtgg
     3241 tgttggtatt ttcatgatgg gcgcaggact ctcttggtat catggaatca tgggattact
     3301 tcatcctcaa ccaatggaat ccctgctatg ggcatattgt attttagcag gatctttggt
     3361 gtctgaagga gcaacacttc tcgttgccat aaatgaactt cgcaggagcg cgcaggccaa
     3421 gggcacgaca ttttataagt atgtcatgga aagtcgtgat ccgagtacaa atgttattct
     3481 attggaggac actgcagctg tcttaggagt gataatagcg gctacctgca tgggccttac
     3541 ctccataaca ggcaatccac tgtatgacag cctgggttcc ttgggtgtag gcaccttact
     3601 aggtgtggtc tcggcattcc tcatctatac caacacagaa gcactcctag gacgctccat
     3661 ccaaccggag caagtgcagc ggcttactga actcctggag agcgacccgt cagtaagggc
     3721 aatccatgat gttaaagcca cagatttggg attagggaaa gtcagattta aggcagaagt
     3781 tgattttgat ggacgagttg ttacgagatc atatttggaa aagcaagatt ttgaccaaat
     3841 gatgcaagaa attcaagaag tgaagactcc tgaacaatta gaagccttta tgcttaaaca
     3901 tggagaaaat attattgata ctctaggagc tgaagtagac agacttgaga aagagctgaa
     3961 aaaacgaaac cctgaagttc ggcacgtaga ccttgagatc ctaggatccg actacaaaga
     4021 ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg
     4081 gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     4141 agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg
     4201 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     4261 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     4321 cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac
     4381 ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc
     4441 ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc
     4501 ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct
     4561 tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg
     4621 accggcgcct acgctagacg ccaccatggt gagcaagggc gaggaggata acatggccat
     4681 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     4741 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     4801 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     4861 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     4921 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     4981 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     5041 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     5101 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     5161 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     5221 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     5281 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     5341 cgagctgtac aagggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga
     5401 ggagaatccc ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     5461 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     5521 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     5581 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     5641 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     5701 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     5761 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     5821 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     5881 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     5941 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     6001 caagcccggt gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6061 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     6121 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     6181 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     6241 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     6301 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     6361 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     6421 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     6481 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     6541 gcctcttccg cgtctccgcc ttcgccctca gacgagtcgg atctcccttt ggccgcctcc
     6601 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6661 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt
     6721 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6781 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6841 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6901 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     6961 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     7021 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7081 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     7141 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7201 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7261 gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7321 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7381 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7441 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7501 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7561 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7621 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7681 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7741 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7801 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7861 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7921 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7981 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8041 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8101 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     8161 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8221 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8281 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8341 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8401 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8461 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8521 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8581 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8641 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8701 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8761 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8821 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8881 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8941 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9001 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9061 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9121 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9181 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9241 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9301 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9361 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9421 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     9481 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     9541 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     9601 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     9661 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     9721 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     9781 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     9841 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     9901 acgacggcca gtgccaagct g
//
