LOCUS V038394 9907 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038394 VERSION V038394 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2303..3967 /label="Slc1a5(NM_009201)" /note="Slc1a5(NM_009201)" /gene="Slc1a5" CDS 3974..4000 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4004..4030 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4034..4060 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 4105..4316 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4329..4597 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4632..5339 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 5349..5402 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 5403..6002 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6003..6590 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6664..6897 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6969..7090 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7109..7244 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7282..7298) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7306..7322 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7330..7360) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7375..7396 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7684..8272) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8443..9303) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9304..9408) /gene="bla" /label="AmpR promoter" primer_bind 9882..9898 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatggcagt ggatccccct aaggctgacc ccaaaggggt 2341 agtagcggtg gattccaccg cgaacggtgg ccccgcgctg ggatccagag aggaccagag 2401 tgcgaaagca ggtggttgct gcggttcccg tgaccgggtg cgccgctgca ttcgcgccaa 2461 cctgctggtg ctgctcacgg tggctgcggt ggtggctggc gtggggctgg ggctgggggt 2521 ctcggcggcg ggcggtgctg acgcgctggg tcccgcgcgc ttgaccgctt tcgccttccc 2581 gggagagctg ctgctgcgtc tgctgaagat gatcatcctg ccgctcgtgg tgtgcagcct 2641 gatcggaggt gcagccagct tggaccctag cgcgctcggt cgtgtgggcg cctgggcgct 2701 gctctttttc ctggtcacca cactgctcgc gtcggcgctc ggcgtgggtt tggccctggc 2761 gctgaagccg ggcgccgccg ttaccgccat cacctccatc aacgactctg ttgtagaccc 2821 ctgtgcccgc agtgcaccaa ccaaagaggt gctggattcc tttctagatc tcgtgaggaa 2881 tattttcccc tccaatctgg tgtctgctgc cttccgctct tttgctacct catatgaacc 2941 caaagacaac tcatgtaaaa taccgcaatc ctgtatccag cgggagatca attcaaccat 3001 ggtccagctt ctctgtgagg tggagggaat gaacatcctg ggcctggtgg tcttcgctat 3061 cgtctttggt gtggctctgc ggaagctggg gcccgagggt gagctgctca ttcgtttctt 3121 caactccttc aatgatgcca ccatggtcct ggtctcctgg attatgtggt acgcacccgt 3181 tggaatcctg ttcctggtgg ccagcaagat tgtggagatg aaagacgtcc gccagctctt 3241 catcagcctc ggcaaataca ttctgtgctg cctgctgggc cacgccatcc acgggctcct 3301 ggttctgcct ctcatctact tcctcttcac ccgcaaaaat ccctatcgat tcctgtgggg 3361 catcatgaca cccctggcca ctgctttcgg gacctcttct agctctgcca ccttgcctct 3421 gatgatgaag tgtgtagagg agaagaatgg tgtggccaaa cacatcagcc ggttcatcct 3481 acccatcggc gccacggtca acatggacgg ggcggcgctg ttccagtgtg tggcggcagt 3541 gttcatcgca caactaaacg gggtgtccct ggacttcgtg aagatcatca ccatcctggt 3601 cacagccaca gcatccagtg tcggtgcggc agggatccct gcagggggcg tcctcaccct 3661 cgccatcatc ctggaagcag tcagcctgcc tgtgaaggac atctccttga tcttggccgt 3721 ggactggcta gtggacaggt cctgtactgt cctcaacgtg gaaggtgatg cttttggggc 3781 tggactgctt cagagttacg tggatcgaac caagatgccg agctcagagc ccgaattgat 3841 ccaggtgaag aacgaggtgt ctctgaatcc actgcccctc gccacagagg aggggaatcc 3901 cctcctgaaa cagtaccagg gacccaccgg ggactccagt gccacgttcg aaaaggaatc 3961 tgtcatggga tcctacccat acgacgtacc tgattatgca acttatcctt atgatgtacc 4021 tgattatgct agctatcctt atgatgtccc agactacgca taggcggccg cgaaggatct 4081 gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt 4141 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg 4201 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa 4261 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga 4321 agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc 4381 cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg 4441 ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga 4501 gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg 4561 tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc 4621 tagacgccac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca 4681 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg 4741 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg 4801 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct 4861 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca 4921 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct 4981 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg 5041 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc 5101 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc 5161 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg 5221 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg 5281 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg 5341 gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc 5401 ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg 5461 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg 5521 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg 5581 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg 5641 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg 5701 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg 5761 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg 5821 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc 5881 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg 5941 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct 6001 gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 6061 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 6121 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 6181 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 6241 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 6301 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 6361 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc 6421 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 6481 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 6541 tccgccttcg ccctcagacg agtcggatct ccctttggcc gcctccccgc ctggtacctt 6601 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 6661 gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt 6721 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 6781 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 6841 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 6901 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 6961 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 7021 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 7081 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca 7141 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 7201 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga 7261 cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 7321 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 7381 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 7441 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 7501 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 7561 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 7621 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 7681 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 7741 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 7801 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 7861 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 7921 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 7981 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 8041 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 8101 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 8161 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 8221 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 8281 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 8341 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 8401 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 8461 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 8521 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 8581 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 8641 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 8701 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 8761 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 8821 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 8881 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 8941 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 9001 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 9061 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 9121 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 9181 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 9241 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 9301 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 9361 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 9421 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 9481 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 9541 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 9601 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 9661 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 9721 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 9781 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 9841 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 9901 caagctg //