LOCUS V019785 9905 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019785 VERSION V019785 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1798..1915 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2005..2208 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2297..3994 /label="Slc15a5(NM_177787)" /note="Slc15a5(NM_177787)" /gene="Slc15a5" CDS 3995..4021 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 4064..4275 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4288..4556 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 4585..4594 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 4615..5277 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 5347..5400 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 5401..6000 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6001..6588 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6662..6895 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6967..7088 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7107..7242 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7280..7296) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7304..7320 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7328..7358) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7373..7394 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7682..8270) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8441..9301) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9302..9406) /gene="bla" /label="AmpR promoter" primer_bind 9880..9896 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc gccaccatgc ccattacaga cttcatcata aatgatgaaa aaacaccctt 2341 agtcttacat ggtggccctg agcagtggaa gacagtgggc ccttatggat gttttcgagt 2401 tggaatctgc ttgcttctgg tggagctgtg tgagaggttc actttcttcg aagtcgtctg 2461 caacatgatc cccttttgca cagggaggct tggctcctac aaccaccagg cagccatgct 2521 caacttgggc tttattggaa cctcagtcct tacccctgtg ttcatgggat ggcttgcgga 2581 tgagtacttc ggaaggaaca aactgatgta catagcctta tctctacatt tcctaggcac 2641 tgccttgtta tccatgctgg cttttccagc ggagaatttc tacagaggcg cttatcctgt 2701 gtttaacaac acgtctgtgg aggaacaggc tgggttgttt catgtagcac tgctgactct 2761 ctgcctgggc acaggaggaa taagggctgt cgtctgtccg ccagatatgt gtggcagtca 2821 ggaacgggaa tcaaagaagc caatgccatt ttgtaactgg gcctcctggt ccgcgaacct 2881 gaatgcagct gttgtctttc tgggaatctc aagcatccag cccttggggt ccggggccct 2941 tggcatcctg cttccttctc tgtccgtgtt caccgccctg gtgactcttt acctgaagca 3001 ctgcgacctg atctatcggc cagagaaccg ttgctccctc ctgacgatcg ccagggcgtt 3061 tgttagggcc ctgaaaacac gctgcctgcc atactgccac tttgggcgag atggatcgag 3121 ttggttagac catgccatgg aaaaacaagg tggacaccac agtgaactcc aagaggaaga 3181 cacaagaaac atctctgccc ttttgcctct cttcagcttc cagatcctgt acagaacgtg 3241 cctcctgcag attccttcag gatattatct tcagaccatg aattccaacc ggaactgggg 3301 tggattttct ctgccgattg cgctaatgaa cgccatcagc cttctgccgt tattaattct 3361 ccctcccttt atggattact tcagcaactg tctacttccc tctaagagag atggaccatt 3421 cctgtcagcg tgcatgattg ctggtaacat ttgtgcagca tcctctgtgg caatggctgg 3481 cttcctagaa atatacagaa aacttgcccg ggagcagtct ccttcaggca aacttttctc 3541 tgtttcctcc atggcctgtg tctgtctggt tccacagtat gtgctgcttg gagtatctga 3601 agtcctggta aaccctgctg tctccatggt aactcacaga gtcatcccag gcgcttccat 3661 ggcttttttg acactgtccc atggatctgc ctgtttcgca ggggcaatgc tgattaagtt 3721 gctttatctc atctcggaag gcaattggtt tccaaacaca ttagacaaag gcaatctaga 3781 gaacttcttc ttcgtgctgg catccttgat gctgctcaac atcctgggac tctggagggc 3841 ttcatggaga tattgtaatc taaatcattt taatgcccag cgcatccgtg gaaatcgttg 3901 tgaagaaact cttctcctca ctgagaagtc tctgaagttt tatggcagta cacagggagc 3961 atcttcaagt attgatcttt gggagacagc cctctaccca tacgacgtac cagattacgc 4021 ttagcggccg caaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 4081 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 4141 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 4201 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 4261 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 4321 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 4381 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 4441 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 4501 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 4561 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 4621 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 4681 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 4741 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 4801 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 4861 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 4921 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 4981 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 5041 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 5101 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 5161 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 5221 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 5281 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 5341 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 5401 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 5461 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 5521 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 5581 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 5641 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 5701 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 5761 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 5821 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 5881 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 5941 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 6001 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 6061 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 6121 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 6181 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 6241 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 6301 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 6361 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc 6421 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 6481 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc 6541 cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta 6601 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 6661 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 6721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 6781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 6841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 6901 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 6961 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 7021 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 7081 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 7141 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 7201 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 7261 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 7321 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 7381 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 7441 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 7501 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 7561 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 7621 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 7681 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 7741 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 7801 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 7861 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 7921 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 7981 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 8041 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 8101 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 8161 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 8221 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 8281 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 8341 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 8401 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 8461 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 8521 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 8581 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 8641 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 8701 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 8761 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 8821 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 8881 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 8941 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 9001 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 9061 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 9121 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 9181 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 9241 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 9301 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 9361 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 9421 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 9481 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 9541 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 9601 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 9661 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 9721 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 9781 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 9841 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 9901 agctg //