LOCUS V038909 8942 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038909 VERSION V038909 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..3018 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="EGFP" CDS 3055..3867 /label="SPI1(NM_001080547)" /note="SPI1(NM_001080547)" /gene="SPI1" promoter 3912..4123 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4136..4404 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4431..5030 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5037..5625 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5699..5932 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6004..6125 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6144..6279 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6317..6333) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6341..6357 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6365..6395) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6410..6431 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6719..7307) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7478..8338) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8339..8443) /gene="bla" /label="AmpR promoter" primer_bind 8917..8933 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg 2341 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 2401 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 2461 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc 2521 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 2581 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 2641 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 2701 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 2761 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 2821 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 2881 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac 2941 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 3001 atggacgagc tgtacaaggg cggcggaggg agtggaggag ggggaagtgg atccatgtta 3061 caggcgtgca aaatggaagg gtttcccctc gtcccccctc agccatcaga agacctggtg 3121 ccctatgaca cggatctata ccaacgccaa acgcacgagt attaccccta tctcagcagt 3181 gatggggaga gccatagcga ccattactgg gacttccacc cccaccacgt gcacagcgag 3241 ttcgagagct tcgccgagaa caacttcacg gagctccaga gcgtgcagcc cccgcagctg 3301 cagcagctct accgccacat ggagctggag cagatgcacg tcctcgatac ccccatggtg 3361 ccaccccatc ccagtcttgg ccaccaggtc tcctacctgc cccggatgtg cctccagtac 3421 ccatccctgt ccccagccca gcccagctca gatgaggagg agggcgagcg gcagagcccc 3481 ccactggagg tgtctgacgg cgaggcggat ggcctggagc ccgggcctgg gctcctgcct 3541 ggggagacag gcagcaagaa gaagatccgc ctgtaccagt tcctgttgga cctgctccgc 3601 agcggcgaca tgaaggacag catctggtgg gtggacaagg acaagggcac cttccagttc 3661 tcgtccaagc acaaggaggc gctggcgcac cgctggggca tccagaaggg caaccgcaag 3721 aagatgacct accagaagat ggcgcgcgcg ctgcgcaact acggcaagac gggcgaggtc 3781 aagaaggtga agaagaagct cacctaccag ttcagcggcg aagtgctggg ccgcgggggc 3841 ctggccgagc ggcgccaccc gccccactga gcggccgcga aggatctgcg atcgctccgg 3901 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 3961 cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 4021 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 4081 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc 4141 tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag 4201 tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag 4261 tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact 4321 cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt 4381 ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag atgaccgagt 4441 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg 4501 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg 4561 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg 4621 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag 4681 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg 4741 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt 4801 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg 4861 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg 4921 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg 4981 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gtcgacaatc 5041 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 5101 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 5161 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 5221 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 5281 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 5341 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 5401 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct 5461 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 5521 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 5581 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga 5641 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 5701 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc 5761 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 5821 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 5881 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 5941 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 6001 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 6061 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 6121 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc 6181 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 6241 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc 6301 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 6361 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 6421 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 6481 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 6541 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 6601 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 6661 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 6721 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 6781 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 6841 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 6901 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 6961 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 7021 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 7081 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 7141 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 7201 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 7261 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 7321 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 7381 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 7441 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 7501 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 7561 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 7621 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 7681 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 7741 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 7801 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 7861 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 7921 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 7981 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 8041 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 8101 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 8161 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 8221 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 8281 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 8341 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 8401 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 8461 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 8521 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 8581 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 8641 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 8701 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 8761 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 8821 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 8881 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc 8941 tg //