LOCUS       V031722                10571 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031722
VERSION     V031722
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4665
                     /label="RIN1(NM_004292)"
                     /note="RIN1(NM_004292)"
                     /gene="RIN1"
     promoter        4730..4941
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4954..5222
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5251..5260
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5281..5943
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6013..6066
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6067..6666
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6667..7254
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7328..7561
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7633..7754
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7773..7908
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7946..7962)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7970..7986
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7994..8024)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8039..8060
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8348..8936)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9107..9967)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9968..10072)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10546..10562
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg aaagccctgg agagtcaggc
     2341 gcgggctctc ctggagcccc cagcccgtcc agcttcacta ctgggcacct ggcgagagaa
     2401 aagccagccc aggacccact gtatgacgtg cccaatgcca gcggcgggca ggcaggcggg
     2461 ccgcagcggc cggggcgcgt tgtgagcctg cgggagcgcc tgctgctcac ccggcccgtg
     2521 tggctgcagc tgcaagccaa cgcagcggcc gcactgcaca tgctgaggac cgagcccccg
     2581 gggacgttcc tcgtgcggaa atctaacacc cgccagtgcc aggccctgtg catgcggttg
     2641 cctgaagcca gtggcccctc cttcgtctcc agccactaca tcctggagag ccctggcggc
     2701 gtctccttgg agggctcgga gctcatgttc ccagacctag tccagctcat ctgtgcctac
     2761 tgccacaccc gggacatcct tctcctcccg ctgcagctcc ccagagccat ccaccacgca
     2821 gccactcaca aagagctgga ggccatctcc catctgggca ttgagttctg gagctcctcc
     2881 ctcaacatca aggctcagcg gggcccggct ggaggcccag tgttgcccca gctgaaggcc
     2941 cggtcccctc aagagctgga ccagggcacc ggagccgcct tgtgcttctt caaccccctg
     3001 ttcccggggg acctagggcc caccaagcgg gagaaattca agagaagctt caaagtgcgc
     3061 gtgtccacag agacctccag ccccctgtct ccacctgccg tgccacctcc ccccgtcccc
     3121 gtgctgccag gggcagtccc cagccagaca gagcggctgc ccccttgcca gctgctacgg
     3181 agggagagct cagtggggta ccgcgtgcca gcaggcagtg gccctagcct tccgcctatg
     3241 ccctccctcc aagaggtgga ctgcggctcc cccagcagct ccgaggagga gggggtgcca
     3301 gggtcccggg ggagcccagc gacctcaccc cacctgggcc gccgacgacc tctgcttcgg
     3361 tccatgagcg ccgccttctg ctccctactg gcaccggagc ggcaggtggg ccgggctgcg
     3421 gcagcactga tgcaggaccg acacacagcc gcgggccagc tggtgcagga cctactgacc
     3481 caggtgcggg ctgggcccga gccccaggag ctgcagggca tccgtcaggc gctgagccgg
     3541 gcccgggcca tgctgagtgc ggagctgggc cctgagaagc tgctgtcgcc taagaggctg
     3601 gaacatgtcc tggagaagtc attgcattgc tctgtgctca agcctctccg gcccatcctg
     3661 gcagcccgcc tgcggcgccg gcttgccgca gacggctccc tgggccgcct agctgagggc
     3721 ctccgcctgg cccgggccca gggccccgga gccttcgggt cccacctgag cctgccctcc
     3781 ccagtagagt tggagcaagt gcgccagaag ctgctgcagc tgctccgcac ctactcaccc
     3841 agcgcccagg tcaagcggct cctgcaggcc tgcaagctgc tctacatggc cctgaggacc
     3901 caggaagggg agggcgcggg tgccgacgag ttcctgcctc tgctgagcct cgtcttggcc
     3961 cactgtgacc ttcctgagct gctgctggag gccgagtaca tgtcggagct gctggagccc
     4021 agcctgctta ctggagaggg tggctactac ctgaccagcc tctctgccag cctggccctg
     4081 ctgagtggcc tgggtcaggc ccacaccctc ccactgagcc ccgtgcagga gctacggcgc
     4141 tccctcagcc tctgggagca gcgccgcctg cctgccaccc actgcttcca gcacctcctc
     4201 cgagtagcct atcaggatcc cagcagtggc tgcacctcca agaccctggc cgtgccccca
     4261 gaagcctcga ttgccaccct gaaccagctc tgtgccacca agttccgagt gacccagccc
     4321 aacacttttg gcctcttcct gtacaaggag cagggctacc accgcctgcc ccctggggcc
     4381 ctggcccaca ggctgcccac cactggctac ctcgtctacc gccgggcgga gtggcctgag
     4441 acccaggggg ctgtgacaga ggaggagggc agtgggcagt cagaggcaag aagcagaggg
     4501 gaggagcaag ggtgccaggg agatggggat gctggggtca aagccagccc cagggacatt
     4561 cgggaacagt ctgagacaac tgctgaaggg ggccagggtc aagcccagga aggccctgct
     4621 cagccagggg aaccagaggc agagggaagc cgggcagcag aggagtaggg atccctgcga
     4681 tatccagcgc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     4741 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     4801 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     4861 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     4921 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     4981 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     5041 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     5101 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     5161 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     5221 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg
     5281 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
     5341 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc
     5401 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac
     5461 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
     5521 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
     5581 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc
     5641 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
     5701 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc
     5761 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac
     5821 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac
     5881 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc
     5941 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc
     6001 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     6061 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     6121 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     6181 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     6241 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     6301 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6361 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6421 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6481 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6541 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6601 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6661 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6721 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6781 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6841 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6901 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6961 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7021 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     7081 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7141 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7201 cgtctccgcc ttcgccctca gacgagtcgg atctcccttt ggccgcctcc ccgcctggta
     7261 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7321 gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact
     7381 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7441 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7501 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7561 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     7621 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7681 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7741 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg
     7801 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7861 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca
     7921 gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7981 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8041 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8101 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8161 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8221 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8281 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8341 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8401 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8461 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8521 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8581 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8641 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8701 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8761 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     8821 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8881 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8941 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9001 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9061 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9121 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9181 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9241 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9301 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9361 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9421 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9481 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9541 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9601 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9661 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9721 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9781 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9841 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9901 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9961 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10021 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10081 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
    10141 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
    10201 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    10261 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
    10321 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
    10381 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
    10441 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    10501 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
    10561 gtgccaagct g
//
