LOCUS V040446 9797 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V040446 VERSION V040446 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..3009 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 3040..4629 /label="RBM39(NM_184234)" /note="RBM39(NM_184234)" /gene="RBM39" CDS 4636..4662 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4666..4692 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4696..4722 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 4767..4978 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4991..5259 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 5286..5885 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5892..6480 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6554..6787 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6859..6980 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6999..7134 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7172..7188) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7196..7212 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7220..7250) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7265..7286 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7574..8162) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8333..9193) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9194..9298) /gene="bla" /label="AmpR promoter" primer_bind 9772..9788 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggtgagc aagggcgagg aggataacat ggccatcatc 2341 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag 2401 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg 2461 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc 2521 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc 2581 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc 2641 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac 2701 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag 2761 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag 2821 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag 2881 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac 2941 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag 3001 ctgtacaagg gaggtggagg tagtggagga ggcggatcca tggcagacga tattgatatt 3061 gaagcaatgc ttgaggctcc ttacaagaag gatgagaaca agttgagcag tgccaacggc 3121 catgaagaac gtagcaaaaa gaggaaaaaa agcaagagca gaagtcgtag tcatgaacga 3181 aagagaagca aaagtaagga acggaagcga agtagagaca gagaaaggaa aaagagcaaa 3241 agccgtgaaa gaaagcgaag tagaagcaaa gagaggcgac ggagccgctc aagaagtcga 3301 gatcgaagat ttagaggccg ctacagaagt ccttactccg gaccaaaatt taacagtgcc 3361 atccgaggaa agattgggtt gcctcatagc atcaaattaa gcagacgacg ttcccgaagc 3421 aaaagtccat tcagaaaaga caagagccct gtgagagaac ctattgataa tttaactcct 3481 gaggaaagag atgcaaggac agtcttctgt atgcagctgg cggcaagaat tcgaccaagg 3541 gatttggaag agtttttctc tacagtagga aaggttcgag atgtgaggat gatttctgac 3601 agaaattcaa gacgttccaa aggaattgct tatgtggagt tcgtcgatgt tagctcagtg 3661 cctctagcaa taggattaac tggccaacga gttttaggcg tgccaatcat agtacaggca 3721 tcacaggcag aaaaaaacag agctgcagca atggcaaaca atttacaaaa gggaagtgct 3781 ggacctatga ggctttatgt gggctcatta cacttcaaca taactgaaga tatgcttcgt 3841 gggatctttg agccttttgg aagaattgaa agtatccagc tgatgatgga cagtgaaact 3901 ggtcgatcca agggatatgg atttattaca ttttctgact cagaatgtgc caaaaaggct 3961 ttggaacaac ttaatggatt tgaactagca ggaagaccaa tgaaagttgg tcatgttact 4021 gaacgtactg atgcttcgag tgctagttca tttttggaca gtgatgaact ggaaaggact 4081 ggaattgatt tgggaacaac tggtcgtctt cagttaatgg caagacttgc agagggtaca 4141 ggtttgcaga ttccgccagc agcacagcaa gctctacaga tgagtggctc tttggcattt 4201 ggtgctgtgg cagaattctc ttttgttata gatttgcaaa caagactttc ccagcagact 4261 gaagcttcag ctttagctgc agctgcctct gttcagccac ttgcaacaca atgtttccaa 4321 ctctctaaca tgtttaaccc tcaaacagaa gaagaagttg gatgggatac cgagattaag 4381 gatgatgtga ttgaagaatg taataaacat ggaggagtta ttcatattta tgttgacaaa 4441 aattcagctc agggcaatgt gtatgtgaag tgcccatcaa ttgctgcagc tattgctgct 4501 gtcaatgcat tgcatggcag gtggtttgct ggtaaaatga taacagcagc atatgtacct 4561 cttccaactt accacaacct gtttcctgat tctatgacag caacacagct actggttcca 4621 agtagacgac tcgagtaccc atacgacgta cctgattatg caacttatcc ttatgatgta 4681 cctgattatg ctagctatcc ttatgatgtc ccagactacg cataggcggc cgcgaaggat 4741 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa 4801 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg 4861 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat 4921 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct 4981 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca 5041 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc 5101 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg 5161 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta 5221 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac 5281 gctagatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg 5341 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc 5401 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 5461 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 5521 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 5581 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 5641 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 5701 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 5761 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 5821 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg 5881 cctgagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 5941 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 6001 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 6061 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 6121 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 6181 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 6241 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc 6301 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 6361 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 6421 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 6481 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 6541 gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct 6601 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 6661 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 6721 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 6781 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 6841 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 6901 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 6961 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca 7021 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 7081 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact 7141 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 7201 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 7261 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 7321 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 7381 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 7441 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 7501 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 7561 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 7621 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 7681 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 7741 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 7801 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 7861 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 7921 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 7981 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 8041 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 8101 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 8161 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 8221 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 8281 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 8341 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 8401 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 8461 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 8521 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 8581 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 8641 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 8701 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 8761 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 8821 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 8881 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 8941 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 9001 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 9061 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 9121 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 9181 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 9241 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 9301 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 9361 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa 9421 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 9481 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact 9541 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca 9601 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt 9661 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg 9721 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga 9781 cggccagtgc caagctg //