LOCUS       V041050                10564 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041050
VERSION     V041050
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4590
                     /label="Plod2(NM_001142916)"
                     /note="Plod2(NM_001142916)"
                     /gene="Plod2"
     CDS             4597..4662
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4706..4917
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4930..5198
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5227..5236
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5257..5919
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5989..6042
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6043..6642
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6643..7231
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7305..7538
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7610..7731
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7771..7906
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7939..7955)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7963..7979
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7987..8017)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8032..8053
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8341..8929)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9100..9960)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9961..10065)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10539..10555
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gggaccgcgg agcgaggccg
     2341 gggcggctga tgcccatgct cgccctgctc tcctgggcgg ccggcctggg cgtggcggag
     2401 gagacgcccg ggcgcatccc tgcagataaa ttattagtca taactgtcgc aacaaaagaa
     2461 aatgatggat tccacagatt tatgaattca gccaagtatt tcaattacac tgtgaaggtt
     2521 cttggtcaag gtcaggagtg gagaggcggt gatggaatga acagtatcgg agggggccag
     2581 aaggtgagat tactgaaaga agctatggag cactacgcca gtcaggaaga tctggtcatc
     2641 ttgtttaccg agtgttttga tgttgtattt gctggtgggc ctgaagaagt tcttaaaaag
     2701 ttccaaaaga caaatcataa aatcgtcttt gcagcggacg ggctgctgtg gccagataag
     2761 cggctggcag acaagtaccc tgttgtgcac attgggaaac gctacctgaa ttctggaggc
     2821 tttattggct atgccccgta catcagccgt ctggtccagc agtggaatct gcaggataat
     2881 gatgatgatc agctctttta cacgaaagtt tacatcgacc cactgaagag ggaagctttt
     2941 aacatcacac tggatcacaa atgcaaaatt ttccaggcct tgaatggagc tacagatgaa
     3001 gttgttttaa agtttgaaaa tggtaaaagc agagtgaaga atacatttta tgaaacactg
     3061 ccagtggcaa ttaatggaaa tgggcccacc aagattctcc tgaattactt tggaaactat
     3121 gttccaaact catggacaca ggaaaatggc tgtgcgcttt gtgatgttga cacaattgac
     3181 ttgtctacag tagatgtccc tcccaaggtt acactgggtg tttttattga acaaccaacc
     3241 ccctttctac ctcgattcct gaacttactg ttaacactgg attatcccaa agaagccctt
     3301 caactcttta ttcataataa agaagtttat catgaaaagg acatcaaagt gtttgttgat
     3361 aaagctaagc atgacatcag ctctataaaa atagtaggac cagaggagaa cctaagtcaa
     3421 gcagaagcca ggaacatggg catggatttc tgccgtcagg atgaaaagtg tgattactac
     3481 tttagtgtgg atgcagatgt tgttttgaca aacccaagaa ctttaaaatt tttgattgaa
     3541 caaaacagga aaatcattgc tcctcttgtg acacgtcatg gaaagttgtg gtccaacttt
     3601 tggggagcac tgagtcctga tgggtactat gctcgctctg aagattacgt ggatattgtt
     3661 caggggaaca gagtaggaat ctggaatgtc ccatatatgg ctaatgtgta cttaattcaa
     3721 ggaaagacac tccgatctga gatgaatgaa aggaactatt ttgtccgtga taagttggat
     3781 cccgatatgg ctctttgccg caatgctaga gatatgacct tacaaaggga aaaagactcc
     3841 cctactccgg aaacattcca aatgctcagc cccccaaagg gtgtgtttat gtacatttct
     3901 aacagacatg aatttggacg gctgatatca actgctaatt acaacacttc ccatcttaac
     3961 aatgacttct ggcagatttt tgaaaatccc gtggattgga aggaaaaata tataaatcgt
     4021 gattattcaa agattttcac tgaaaatata gtcgagcagc cttgtccaga tgtcttctgg
     4081 tttcccatat tttctgaacg agcctgtgat gagttggtag aagaaatgga gcattatggc
     4141 aagtggtccg gcggaaagca tcacgatagc cgcatatcgg gtggttatga aaatgtcccg
     4201 actgacgata ttcatatgaa gcagatcggg ctggagaatg tttggcttca tttcatccga
     4261 gagttcattg ctccagtcac actgaaggtc ttcgcaggct attatacaaa gggatttgca
     4321 ctgctgaatt ttgtagtaaa gtactcacct gaaagacagc gctcgctccg tccccaccac
     4381 gatgcctcaa cctttaccat caacattgct ctgaataatg taggagagga ttttcaggga
     4441 ggtggatgca aatttctgag gtataattgc tccattgagt ccccacgaaa aggctggagc
     4501 ttcatgcatc ctgggaggct cacacatttg cacgaaggac ttcctgttaa aaatggaaca
     4561 cgatacattg cagtgtcatt tatagatccc ggatccgact acaaagacca tgacggtgat
     4621 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc gcgaaggatc
     4681 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     4741 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     4801 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     4861 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     4921 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     4981 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     5041 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     5101 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     5161 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     5221 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
     5281 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
     5341 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
     5401 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
     5461 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
     5521 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
     5581 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
     5641 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
     5701 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
     5761 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
     5821 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
     5881 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc
     5941 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga
     6001 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
     6061 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     6121 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     6181 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     6241 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     6301 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     6361 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     6421 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     6481 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     6541 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     6601 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac
     6661 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6721 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6781 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6841 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6901 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6961 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     7021 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     7081 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     7141 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     7201 agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa
     7261 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     7321 ctcccaacga agataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     7381 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7441 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7501 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     7561 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     7621 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7681 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7741 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     7801 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     7861 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga
     7921 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7981 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8041 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8101 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8161 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8221 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8281 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8341 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8401 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8461 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8521 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8581 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8641 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8701 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8761 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     8821 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8881 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8941 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9001 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9061 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9121 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9181 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9241 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9301 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9361 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9421 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9481 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9541 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9601 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9661 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9721 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9781 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9841 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9901 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9961 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10021 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10081 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    10141 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    10201 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    10261 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
    10321 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    10381 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    10441 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    10501 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
    10561 gctg
//
