LOCUS       V025052                12734 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025052
VERSION     V025052
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6777
                     /label="Pla2r1(NM_008867)"
                     /note="Pla2r1(NM_008867)"
                     /gene="Pla2r1"
     CDS             6784..6849
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6893..7104
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7117..7385
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7414..7423
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7444..8106
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8176..8229
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8230..8829
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8830..9417
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9491..9724
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9796..9917
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9936..10071
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10109..10125)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10133..10149
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10157..10187)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10202..10223
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10511..11099)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11270..12130)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12131..12235)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12709..12725
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg tgcaatggtt agcgatgctg
     2341 cagctgctct ggctgcaaca gctgctgctg ctggggattc accagggcat cgcccaagat
     2401 ttgactcaca tccaggaacc gtccctcgag tggcgtgata aaggaatttt cattatccag
     2461 agcgagagcc tgaagacatg cattcaggca ggtaaatctg tactgaccct ggagaactgc
     2521 aagcagccaa acgagcacat gctgtggaaa tgggtttccg acgaccacct ctttaatgtg
     2581 ggaggcagtg gctgcctagg cctgaacata tctgctctgg agcaaccatt aaagctctat
     2641 gaatgtgatt ccaccctcat ttccctgaga tggcactgtg acaggaagat gatcgagggc
     2701 ccactccagt acaaggtcca ggtgaagtct gacaacacag tggtggcaag aaaacagatc
     2761 cataggtgga tcgcctacac gtccagtggt ggggacattt gtgagcatcc tagcagagat
     2821 ttatatacgt taaaagggaa tgcccatggg atgccttgcg tgtttccttt ccagttcaaa
     2881 ggtcactggc accatgactg tatccgagaa gggcagaaag agcacctgct gtggtgtgcc
     2941 accacaagcc gatacgaaga agacgagaag tggggattct gcccagatcc tacctctatg
     3001 aaggtgttct gtgatgctac gtggcaaagg aatggcagtt cacgcatttg ctaccagttc
     3061 aacctgcttt cgtctctgtc ttggaaccag gcccattctt catgcctgat gcaaggaggt
     3121 gctttgctaa gtattgcaga tgaagatgaa gaagatttca taaggaagca cttgagcaag
     3181 gtagtcaagg aagtgtggat tggtttgaac cagctggatg agaaggctgg ctggcagtgg
     3241 tctgatggaa caccactcag ctacctgaac tggagccaag agataactcc tgggccattt
     3301 gttgagcatc actgtggaac cctggaggtt gtttcagctg cctggaggag cagggactgc
     3361 gagtccacct tgccttatat atgcaaacgg gatctaaacc acactgcgca gggaatcctg
     3421 gaaaaggact cctggaaata ccatgccact cactgtgatc ctgactggac tcccttcaat
     3481 cgtaaatgct acaaacttaa gaaagacaga aagagctggc ttggggctct gcactcttgc
     3541 caatcgaatg acagcgtgtt gatggatgta gcttcgttgg cagaggtgga gtttcttgta
     3601 agcctcctta gggatgaaaa tgcatcagaa acgtggattg gtttgagtag caataaaatc
     3661 ccagtctcct ttgagtggtc cagtggctcc tctgtcatct ttactaactg gtaccctctg
     3721 gagcctcgaa ttcttccaaa cagaagacaa ctgtgtgtct cagccgagga atctgacgga
     3781 cgctggaaag tgaaagattg taaagaaaga ctcttttata tttgtaagaa agcagggcaa
     3841 gtccctgctg atgagcagtc cggatgtcca gcgggctggg agagacacgg cagattttgt
     3901 tacaaaatcg acacagtcct gcgaagcttt gaggaggctt cgagtggcta ttactgctct
     3961 cctgcgctcc tgaccatcac cagccgattt gaacaagctt ttattaccag tttgatcagt
     4021 agtgtggcag aaaaggacag ttatttttgg attgctcttc aagaccaaaa caatacagga
     4081 gagtacactt ggaagaccgt ggggcagagg gagccggtgc agtacacata ctggaacacg
     4141 cgtcaaccca gcaatcgtgg tggctgcgtt gttgtacgag gaggaagttc acttggtcgc
     4201 tgggaagtga aggattgtag cgactttaag gcaatgtctc tgtgcaagac accagtcaaa
     4261 atctgggaga aaacagagct tgaagagaga tggccgtttc acccctgcta tatggattgg
     4321 gaatcagcca ctggtctggc cagttgcttc aaggtgtttc atagcgaaaa ggtcctgatg
     4381 aaaagatcgt ggagagaagc agaagcattc tgtgaggagt ttggggctca cctcgcaagc
     4441 tttgcccata tagaggaaga aaatttcgtg aatgagcttc tacattcaaa atttaattgg
     4501 acacaggaaa ggcagttctg gattggattt aatagaagga acccactgaa tgctggttct
     4561 tgggcgtggt ctgacgggtc acctgttgtc tcttcatttt tagacaatgc ttattttgaa
     4621 gaagatgcaa aaaattgtgc tgtgtataag gcaaacaaaa cattgttgcc ctcgaactgt
     4681 gcctccaaac acgagtggat atgcagaatt ccaagagatg tgagacccaa gtttccagac
     4741 tggtaccagt acgatgcgcc gtggctcttc tatcagaacg ccgagtacct cttccacacc
     4801 cacccagcgg agtgggccac ctttgagttt gtctgcggct ggctgcgaag tgacttcctc
     4861 acaatttatt ctgctcagga gcaagaattc atccacagca aaatcaaagg gctgacaaag
     4921 tatggtgtga agtggtggat cggacttgaa gaaggaggag ccagagatca aattcagtgg
     4981 agtaacgggt cgcccgtgat attccagaac tgggacaaag gaagagaaga aagagtggat
     5041 agccagagaa agcgatgtgt cttcatatct tccataacag ggctctgggg gactgagaac
     5101 tgctctgttc ctttgcccag catctgtaag cgagtgaaga tatgggtcat cgagaaggag
     5161 aagccgccga cacagcctgg gacatgcccc aaggggtggc tgtattttaa ctataagtgc
     5221 ttcctggtga caattcccaa ggaccctcgg gagctgaaga cctggacagg tgctcaggaa
     5281 ttctgtgtcg ccaagggtgg gacgctggtc tccatcaaaa gtgaattgga acaagctttt
     5341 attactatga atctttttgg ccagaccacg aatgtgtgga tagggttaca aagtactaat
     5401 catgagaaat gggtaaatgg gaagcctctg gtgtactcta actggtctcc atcggatata
     5461 ataaatatac caagttataa caccactgaa tttcaaaagc atattcctct ctgtgccctc
     5521 atgtcaagta atcctaattt ccattttact ggaaaatggt atttcgatga ctgtgggaaa
     5581 gaaggctatg gatttgtctg tgaaaaaatg caagacaccc ttgaacacca tgtaaacgtg
     5641 tctgatacgt ctgcaatccc cagtacgtta gaatatggaa acagaactta taagataatc
     5701 cgtggtaata tgacttggta tgcagccgga aagtcctgcc ggatgcacag agcagaactg
     5761 gccagcattc cagatgcgtt tcaccaggcc ttcctcacgg ttctcctcag caggctggga
     5821 cacacccact ggattggact ctccaccaca gacaatggtc agacttttga ctggtcggac
     5881 ggcaccaaat cccctttcac ctattggaag gatgaggagt cggcctttct tggtgactgc
     5941 gcttttgctg acaccaacgg acgctggcat agcacagcct gcgagtcatt tctgcaaggc
     6001 gccatttgtc acgtagtcac tgaaacaaag gcatttgagc acccagggct gtgctcagaa
     6061 acatcagttc cctggataaa atttaaaggt aactgctaca gtttttccac agtcctggac
     6121 agcaggagtt ttgaagatgc tcatgaattt tgcaaaagtg aaggatctaa tctgttggca
     6181 atcagggatg cggctgaaaa ttcatttctc ctggaagagc ttcttgcgtt tgggtcttct
     6241 gtgcagatgg tttggttgaa tgctcagttt gataataaca acaaaacctt aaggtggttt
     6301 gacggaacac ccacagagca gtccaactgg ggccttcgga agccagacat ggaccacctc
     6361 aagccacatc cgtgtgttgt cctgaggatc ccggaaggaa tatggcactt taccccatgc
     6421 gaggacaaaa aggggttcat atgtaaaatg gaagcaggca tcccagctgt aacggcgcag
     6481 ccagagaaag gactaagcca cagcatcgtt cctgtcacag taacgctgac gttgataata
     6541 gccctgggca ttttcatgct ttgcttctgg atatataagc agaaaagtga catcttccag
     6601 agactcacgg ggtctcgggg ttcttactac cccacactca acttcagcac agcacattta
     6661 gaagagaaca ttctcatttc tgatcttgag aagaacacca atgatgaaga agttagagat
     6721 gctccagcta cagagagcaa aagaggccac aaagggcgtc ctatctgcat ttcccctgga
     6781 tccgactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac
     6841 gatgacaagt agcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag
     6901 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     6961 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     7021 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     7081 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     7141 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     7201 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     7261 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     7321 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     7381 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc
     7441 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag
     7501 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc
     7561 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc
     7621 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac
     7681 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc
     7741 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc
     7801 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag
     7861 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg
     7921 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc
     7981 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg
     8041 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc
     8101 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc
     8161 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
     8221 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     8281 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     8341 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     8401 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     8461 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     8521 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     8581 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     8641 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     8701 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     8761 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     8821 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     8881 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     8941 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     9001 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     9061 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     9121 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     9181 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     9241 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     9301 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     9361 ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttggccgcc tccccgcctg
     9421 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     9481 aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt
     9541 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     9601 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     9661 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     9721 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     9781 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     9841 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9901 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt
     9961 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
    10021 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt
    10081 gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
    10141 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    10201 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
    10261 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    10321 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    10381 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    10441 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    10501 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    10561 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    10621 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    10681 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    10741 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    10801 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    10861 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    10921 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
    10981 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    11041 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    11101 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    11161 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    11221 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    11281 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    11341 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    11401 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    11461 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    11521 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    11581 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    11641 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    11701 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    11761 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    11821 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    11881 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    11941 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    12001 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    12061 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    12121 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    12181 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    12241 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
    12301 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
    12361 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
    12421 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
    12481 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
    12541 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
    12601 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
    12661 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
    12721 ccagtgccaa gctg
//
