LOCUS V045318 9604 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V045318 VERSION V045318 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2303..3664 /label="MYC(NM_002467)" /note="MYC(NM_002467)" /gene="MYC" CDS 3671..3697 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3701..3727 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3731..3757 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 3802..4013 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4026..4294 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4329..5036 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 5046..5099 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 5100..5699 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5700..6287 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6361..6594 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6666..6787 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6806..6941 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6979..6995) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7003..7019 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7027..7057) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7072..7093 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7381..7969) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8140..9000) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9001..9105) /gene="bla" /label="AmpR promoter" primer_bind 9579..9595 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatggattt ttttcgggta gtggaaaacc agcagcctcc 2341 cgcgacgatg cccctcaacg ttagcttcac caacaggaac tatgacctcg actacgactc 2401 ggtgcagccg tatttctact gcgacgagga ggagaacttc taccagcagc agcagcagag 2461 cgagctgcag cccccggcgc ccagcgagga tatctggaag aaattcgagc tgctgcccac 2521 cccgcccctg tcccctagcc gccgctccgg gctctgctcg ccctcctacg ttgcggtcac 2581 acccttctcc cttcggggag acaacgacgg cggtggcggg agcttctcca cggccgacca 2641 gctggagatg gtgaccgagc tgctgggagg agacatggtg aaccagagtt tcatctgcga 2701 cccggacgac gagaccttca tcaaaaacat catcatccag gactgtatgt ggagcggctt 2761 ctcggccgcc gccaagctcg tctcagagaa gctggcctcc taccaggctg cgcgcaaaga 2821 cagcggcagc ccgaaccccg cccgcggcca cagcgtctgc tccacctcca gcttgtacct 2881 gcaggatctg agcgccgccg cctcagagtg catcgacccc tcggtggtct tcccctaccc 2941 tctcaacgac agcagctcgc ccaagtcctg cgcctcgcaa gactccagcg ccttctctcc 3001 gtcctcggat tctctgctct cctcgacgga gtcctccccg cagggcagcc ccgagcccct 3061 ggtgctccat gaggagacac cgcccaccac cagcagcgac tctgaggagg aacaagaaga 3121 tgaggaagaa atcgatgttg tttctgtgga aaagaggcag gctcctggca aaaggtcaga 3181 gtctggatca ccttctgctg gaggccacag caaacctcct cacagcccac tggtcctcaa 3241 gaggtgccac gtctccacac atcagcacaa ctacgcagcg cctccctcca ctcggaagga 3301 ctatcctgct gccaagaggg tcaagttgga cagtgtcaga gtcctgagac agatcagcaa 3361 caaccgaaaa tgcaccagcc ccaggtcctc ggacaccgag gagaatgtca agaggcgaac 3421 acacaacgtc ttggagcgcc agaggaggaa cgagctaaaa cggagctttt ttgccctgcg 3481 tgaccagatc ccggagttgg aaaacaatga aaaggccccc aaggtagtta tccttaaaaa 3541 agccacagca tacatcctgt ccgtccaagc agaggagcaa aagctcattt ctgaagagga 3601 cttgttgcgg aaacgacgag aacagttgaa acacaaactt gaacagctac ggaactcttg 3661 tgcgggatcc tacccatacg acgtacctga ttatgcaact tatccttatg atgtacctga 3721 ttatgctagc tatccttatg atgtcccaga ctacgcatag gcggccgcga aggatctgcg 3781 atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 3841 ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa 3901 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 3961 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc 4021 ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac 4081 gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg 4141 tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc 4201 tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct 4261 ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag 4321 acgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc 4381 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg 4441 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc 4501 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg 4561 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt 4621 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc 4681 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg 4741 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg 4801 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact 4861 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct 4921 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac 4981 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagggat 5041 ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta 5101 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac 5161 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc 5221 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca 5281 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga 5341 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt 5401 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc 5461 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca 5521 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg 5581 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg 5641 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa 5701 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 5761 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 5821 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 5881 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 5941 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 6001 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 6061 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg 6121 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 6181 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc 6241 gccttcgccc tcagacgagt cggatctccc tttggccgcc tccccgcctg gtacctttaa 6301 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 6361 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc 6421 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 6481 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 6541 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 6601 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 6661 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 6721 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 6781 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc 6841 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 6901 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg 6961 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 7021 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 7081 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 7141 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 7201 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 7261 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 7321 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 7381 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 7441 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 7501 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 7561 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 7621 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 7681 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 7741 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 7801 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 7861 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 7921 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 7981 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 8041 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 8101 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 8161 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 8221 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 8281 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 8341 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 8401 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 8461 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 8521 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 8581 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 8641 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 8701 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 8761 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 8821 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 8881 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 8941 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 9001 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 9061 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 9121 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 9181 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 9241 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 9301 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 9361 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 9421 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 9481 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 9541 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 9601 gctg //