LOCUS       V042814                10605 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042814
VERSION     V042814
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4666
                     /label="MARK2(NM_001039469)"
                     /note="MARK2(NM_001039469)"
                     /gene="MARK2"
     CDS             4673..4699
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4703..4729
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4733..4759
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4803..5014
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5027..5295
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5330..6037
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             6047..6100
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6101..6700
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6701..7288
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7362..7595
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7667..7788
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7807..7942
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7980..7996)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8004..8020
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8028..8058)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8073..8094
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8382..8970)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9141..10001)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10002..10106)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10580..10596
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgtccag cgctcggacc cccctaccca cgctgaacga
     2341 gagggacacg gagcagccca ccttgggaca ccttgactcc aagcccagca gtaagtccaa
     2401 catgattcgg ggccgcaact cagccacctc tgctgatgag cagccccaca ttggaaacta
     2461 ccggctcctc aagaccattg gcaagggtaa ttttgccaag gtgaagttgg cccgacacat
     2521 cctgactggg aaagaggtag ctgtgaagat cattgacaag actcaactga actcctccag
     2581 cctccagaaa ctattccgcg aagtaagaat aatgaaggtt ttgaatcatc ccaacatagt
     2641 taaattattt gaagtgattg agactgagaa aacgctctac cttgtcatgg agtacgctag
     2701 tggcggagag gtatttgatt acctagtggc tcatggcagg atgaaagaaa aagaggctcg
     2761 agccaaattc cgccagatag tgtctgctgt gcagtactgt caccagaagt ttattgtcca
     2821 tagagactta aaggcagaaa acctgctctt ggatgctgat atgaacatca agattgcaga
     2881 ctttggcttc agcaatgaat tcacctttgg gaacaagctg gacaccttct gtggcagtcc
     2941 cccttatgct gccccagaac tcttccaggg caaaaaatat gatggacccg aggtggatgt
     3001 gtggagccta ggagttatcc tctatacact ggtcagcgga tccctgcctt ttgatggaca
     3061 gaacctcaag gagctgcggg aacgggtact gaggggaaaa taccgtattc cattctacat
     3121 gtccacggac tgtgaaaacc tgcttaagaa atttctcatt cttaatccca gcaagagagg
     3181 cactttagag caaatcatga aagatcgatg gatgaatgtg ggtcacgaag atgatgaact
     3241 aaagccttac gtggagccac tccctgacta caaggacccc cggcggacag agctgatggt
     3301 gtccatgggt tatacacggg aagagatcca ggactcgctg gtgggccaga gatacaacga
     3361 ggtgatggcc acctatctgc tcctgggcta caagagctcc gagctggaag gcgacaccat
     3421 caccctgaaa ccccggcctt cagctgatct gaccaatagc agcgccccat ccccatccca
     3481 caaggtacag cgcagcgtgt cggccaatcc caagcagcgg cgcttcagcg accaggcagc
     3541 tggtcctgcc attcccacct ctaattctta ctctaagaag actcagagta acaacgcaga
     3601 aaataagcgg cctgaggagg accgggagtc agggcggaaa gccagcagca cagccaaggt
     3661 gcctgccagc cccctgcccg gtctggagag gaagaagacc accccaaccc cctccacgaa
     3721 cagcgtcctc tccaccagca caaatcgaag caggaattcc ccacttttgg agcgggccag
     3781 cctcggccag gcctccatcc agaatggcaa agacagccta accatgccag ggtcccgggc
     3841 ctccacggct tctgcttctg ccgcagtctc tgcggcccgg ccccgccagc accagaaatc
     3901 catgtcggcc tccgtgcacc ccaacaaggc ctctgggctg ccccccacgg agagtaactg
     3961 tgaggtgccg cggcccagca cagcccccca gcgtgtccct gttgcctccc catccgccca
     4021 caacatcagc agcagtggtg gagccccaga ccgaactaac ttcccccggg gtgtgtccag
     4081 ccgaagcacc ttccatgctg ggcagctccg acaggtgcgg gaccagcaga atttgcccta
     4141 cggtgtgacc ccagcctctc cctctggcca cagccagggc cggcgggggg cctctgggag
     4201 catcttcagc aagttcacct ccaagtttgt acgcagaaat ctgtctttca ggtttgccag
     4261 aaggaacctg aatgaacctg aaagcaaaga ccgagtggag acgctcagac ctcacgtggt
     4321 gggcagtggc ggcaacgaca aagaaaagga agaatttcgg gaggccaagc cccgctccct
     4381 ccgcttcacg tggagtatga agaccacgag ctccatggag cccaacgaga tgatgcggga
     4441 gatccgcaag gtgctggacg cgaacagctg ccagagcgag ctgcatgaga agtacatgct
     4501 gctgtgcatg cacggcacgc cgggccacga ggacttcgtg cagtgggaga tggaggtgtg
     4561 caaactgccg cggctctctc tcaacggggt tcgatttaag cggatatcgg gcacctccat
     4621 ggccttcaaa aacattgcct ccaaaatagc caacgagctg aagcttggat cctacccata
     4681 cgacgtacct gattatgcaa cttatcctta tgatgtacct gattatgcta gctatcctta
     4741 tgatgtccca gactacgcat agcggccgcg aaggatctgc gatcgctccg gtgcccgtca
     4801 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     4861 aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     4921 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     4981 tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc
     5041 tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct
     5101 gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct
     5161 caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct
     5221 ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct
     5281 gcgccgttac agatccaagc tgtgaccggc gcctacgcta gacgccacca tggtgagcaa
     5341 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     5401 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     5461 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     5521 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     5581 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     5641 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     5701 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     5761 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     5821 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     5881 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     5941 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     6001 ccgccactcc accggcggca tggacgagct gtacaaggga tctcgcgagg gcagaggaag
     6061 tcttctaaca tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac
     6121 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     6181 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     6241 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     6301 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     6361 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     6421 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     6481 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     6541 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     6601 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     6661 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa
     6721 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6781 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6841 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6901 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6961 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7021 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7081 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     7141 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7201 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag
     7261 tcggatctcc ctttggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc
     7321 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     7381 ccaacgaaaa taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     7441 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7501 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7561 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     7621 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     7681 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7741 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     7801 ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7861 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     7921 tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg
     7981 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8041 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     8101 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8161 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8221 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8281 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8341 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8401 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8461 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8521 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8581 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8641 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8701 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8761 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8821 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8881 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8941 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9001 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9061 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9121 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9181 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9241 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9301 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9361 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9421 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9481 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9541 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9601 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9661 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9721 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9781 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9841 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9901 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9961 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    10021 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10081 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
    10141 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
    10201 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
    10261 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
    10321 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
    10381 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
    10441 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
    10501 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
    10561 ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//
