LOCUS       V040793                10307 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040793
VERSION     V040793
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4308
                     /label="LMNA(NM_170707)"
                     /note="LMNA(NM_170707)"
                     /gene="LMNA"
     CDS             4315..4341
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4345..4371
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4375..4401
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4466..4677
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4690..4958
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4987..4996
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5017..5679
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5749..5802
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5803..6402
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6403..6990
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7064..7297
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7369..7490
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7509..7644
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7682..7698)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7706..7722
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7730..7760)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7775..7796
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8084..8672)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8843..9703)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9704..9808)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10282..10298
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg agaccccgtc ccagcggcgc
     2341 gccacccgca gcggggcgca ggccagctcc actccgctgt cgcccacccg catcacccgg
     2401 ctgcaggaga aggaggacct gcaggagctc aatgatcgct tggcggtcta catcgaccgt
     2461 gtgcgctcgc tggaaacgga gaacgcaggg ctgcgccttc gcatcaccga gtctgaagag
     2521 gtggtcagcc gcgaggtgtc cggcatcaag gccgcctacg aggccgagct cggggatgcc
     2581 cgcaagaccc ttgactcagt agccaaggag cgcgcccgcc tgcagctgga gctgagcaaa
     2641 gtgcgtgagg agtttaagga gctgaaagcg cgcaatacca agaaggaggg tgacctgata
     2701 gctgctcagg ctcggctgaa ggacctggag gctctgctga actccaagga ggccgcactg
     2761 agcactgctc tcagtgagaa gcgcacgctg gagggcgagc tgcatgatct gcggggccag
     2821 gtggccaagc ttgaggcagc cctaggtgag gccaagaagc aacttcagga tgagatgctg
     2881 cggcgggtgg atgctgagaa caggctgcag accatgaagg aggaactgga cttccagaag
     2941 aacatctaca gtgaggagct gcgtgagacc aagcgccgtc atgagacccg actggtggag
     3001 attgacaatg ggaagcagcg tgagtttgag agccggctgg cggatgcgct gcaggaactg
     3061 cgggcccagc atgaggacca ggtggagcag tataagaagg agctggagaa gacttattct
     3121 gccaagctgg acaatgccag gcagtctgct gagaggaaca gcaacctggt gggggctgcc
     3181 cacgaggagc tgcagcagtc gcgcatccgc atcgacagcc tctctgccca gctcagccag
     3241 ctccagaagc agctggcagc caaggaggcg aagcttcgag acctggagga ctcactggcc
     3301 cgtgagcggg acaccagccg gcggctgctg gcggaaaagg agcgggagat ggccgagatg
     3361 cgggcaagga tgcagcagca gctggacgag taccaggagc ttctggacat caagctggcc
     3421 ctggacatgg agatccacgc ctaccgcaag ctcttggagg gcgaggagga gaggctacgc
     3481 ctgtccccca gccctacctc gcagcgcagc cgtggccgtg cttcctctca ctcatcccag
     3541 acacagggtg ggggcagcgt caccaaaaag cgcaaactgg agtccactga gagccgcagc
     3601 agcttctcac agcacgcacg cactagcggg cgcgtggccg tggaggaggt ggatgaggag
     3661 ggcaagtttg tccggctgcg caacaagtcc aatgaggacc agtccatggg caattggcag
     3721 atcaagcgcc agaatggaga tgatcccttg ctgacttacc ggttcccacc aaagttcacc
     3781 ctgaaggctg ggcaggtggt gacgatctgg gctgcaggag ctggggccac ccacagcccc
     3841 cctaccgacc tggtgtggaa ggcacagaac acctggggct gcgggaacag cctgcgtacg
     3901 gctctcatca actccactgg ggaagaagtg gccatgcgca agctggtgcg ctcagtgact
     3961 gtggttgagg acgacgagga tgaggatgga gatgacctgc tccatcacca ccacggctcc
     4021 cactgcagca gctcggggga ccccgctgag tacaacctgc gctcgcgcac cgtgctgtgc
     4081 gggacctgcg ggcagcctgc cgacaaggca tctgccagcg gctcaggagc ccaggtgggc
     4141 ggacccatct cctctggctc ttctgcctcc agtgtcacgg tcactcgcag ctaccgcagt
     4201 gtggggggca gtgggggtgg cagcttcggg gacaatctgg tcacccgctc ctacctcctg
     4261 ggcaactcca gcccccgaac ccagagcccc cagaactgca gcatcatgct cgagtaccca
     4321 tacgacgtac ctgattatgc aacttatcct tatgatgtac ctgattatgc tagctatcct
     4381 tatgatgtcc cagactacgc atagggatcc ctgcgatatc cagcgcggcc gcgaaggatc
     4441 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     4501 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     4561 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     4621 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     4681 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     4741 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     4801 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     4861 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     4921 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     4981 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
     5041 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
     5101 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
     5161 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
     5221 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
     5281 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
     5341 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
     5401 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
     5461 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
     5521 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
     5581 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
     5641 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc
     5701 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga
     5761 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
     5821 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     5881 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     5941 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     6001 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     6061 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     6121 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     6181 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     6241 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     6301 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     6361 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac
     6421 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6481 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6541 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6601 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6661 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6721 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6781 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     6841 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     6901 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg
     6961 agtcggatct ccctttggcc gcctccccgc ctggtacctt taagaccaat gacttacaag
     7021 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7081 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7141 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7201 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7261 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7321 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     7381 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7441 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7501 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7561 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     7621 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat
     7681 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7741 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7801 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7861 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7921 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7981 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8041 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8101 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8161 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8221 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8281 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8341 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8401 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8461 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8521 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8581 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8641 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8701 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8761 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8821 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8881 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8941 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9001 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9061 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9121 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9181 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9241 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9301 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9361 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9421 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9481 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9541 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9601 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9661 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9721 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9781 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     9841 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     9901 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     9961 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
    10021 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
    10081 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
    10141 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
    10201 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    10261 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
