LOCUS       V046512                12213 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046512
VERSION     V046512
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..6265
                     /label="KDM3A(NM_018433)"
                     /note="KDM3A(NM_018433)"
                     /gene="KDM3A"
     CDS             6272..6361
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        6411..6622
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6635..6903
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6938..7645
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             7655..7708
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7709..8308
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8309..8896
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8970..9203
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9275..9396
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9415..9550
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9588..9604)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9612..9628
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9636..9666)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9681..9702
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9990..10578)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10749..11609)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11610..11714)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12188..12204
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggtgct cacgctcgga gaaagttggc cggtattggt
     2341 ggggaggagg tttctcagtc tgtccgcagc cgacggcagc gatggcagcc acgacagctg
     2401 ggacgtggag cgcgtcgccg agtggccctg gctctccggg accattcgag ctgtttccca
     2461 caccgacgtt accaagaagg atctgaaggt gtgtgtggaa tttgatgggg aatcttggag
     2521 gaaaagaaga tggatagaag tctacagcct tctaaggaga gcatttttag tagaacataa
     2581 tttggtttta gctgaacgaa agtcacctga aatttctgaa cgaattgtac agtggcctgc
     2641 aataacgtac aaacctctgt tggacaaagc tggtttggga tccataactt ctgttcgctt
     2701 tctgggagat caacaaagag tatttctttc taaagacctt ttgaagccta tacaggatgt
     2761 aaacagtctt cgactttctc ttacggataa tcagattgtc agtaaagaat ttcaagcttt
     2821 gattgtgaag catttagatg aaagccatct tttaaaaggt gacaaaaact tagttggttc
     2881 agaagtaaaa atttatagct tggacccatc tactcagtgg ttttcagcaa ccgttataaa
     2941 tggaaaccca gcatcaaaaa ctcttcaagt caactgtgag gagattccag cactgaaaat
     3001 tgttgatccg tcactgattc atgttgaagt tgtacacgat aaccttgtga catgtggtaa
     3061 ttctgcaaga attggagctg taaaacgcaa gtcttctgag aataatggaa ccctggtttc
     3121 caaacaagca aaatcttgct ctgaggcctc tcccagtatg tgtcctgtgc agtctgtacc
     3181 tacaacagtt tttaaggaga tactgcttgg ctgtactgcg gcaactccac ctagtaagga
     3241 cccaagacag caaagtactc cccaggctgc caactctcca cctaaccttg gagcaaaaat
     3301 tcctcaagga tgtcataaac aaagtttacc agaggaaatt tcttcctgtc taaatacaaa
     3361 gtctgaagct ctgagaacaa aaccagatgt ctgcaaagca gggttgctct caaagtcctc
     3421 tcagattgga actggagact tgaaaattct gactgagcca aaaggcagct gtactcagcc
     3481 taagacaaac actgatcagg aaaacagatt ggagtctgtt ccacaagcat tgactggcct
     3541 tcctaaggag tgcttaccta caaaggcttc ttctaaggca gaattggaaa ttgccaatcc
     3601 tcctgaactg cagaagcacc tagaacatgc accttcccca tcggatgttt caaatgcacc
     3661 agaagtgaaa gcaggtgtca atagtgatag ccctaataac tgttcaggaa aaaaggtaga
     3721 accttcagct ttagcttgcc gatcacagaa tttaaaggaa tcttcagtaa aagtagataa
     3781 tgaaagctgt tgttcaagaa gcaacaataa aatccagaat gccccatcca ggaagtcggt
     3841 tttgacagac ccagctaaac tcaaaaagct gcaacagagt ggcgaggcct tcgtacagga
     3901 tgattcttgt gtgaacatcg tggcacagtt gcctaaatgc cgagagtgtc gcttggacag
     3961 tctccgcaag gataaggagc aacagaagga ctcacctgtg ttttgccgct tctttcactt
     4021 caggaggtta caattcaaca aacatggtgt gttgcgggta gaaggcttct taacaccaaa
     4081 caagtatgac aatgaagcaa ttggcttgtg gttaccttta accaaaaacg ttgtggggat
     4141 tgatttggac acagcaaagt acatcttggc caacattgga gaccacttct gtcaaatggt
     4201 gatttctgaa aaggaagcta tgtcaactat tgagccacac agacaggttg cttggaagcg
     4261 agctgtcaaa ggtgttcgag aaatgtgtga tgtgtgcgac accaccatct tcaacctgca
     4321 ctgggtgtgt cctcggtgtg ggtttggagt atgtgtggac tgctaccgga tgaagagaaa
     4381 gaattgccaa cagggtgctg cttacaagac tttctcttgg ctaaaatgtg tgaagagtca
     4441 gatacatgaa ccagagaact taatgcccac acagatcatt cctggaaaag cactctatga
     4501 tgttggagac attgttcatt ctgtaagagc gaaatgggga ataaaggcaa actgcccttg
     4561 ttcaaacagg caattcaaac tcttttcaaa gccagcctca aaggaagacc taaaacagac
     4621 ttctttagct ggagaaaaac cgactcttgg tgcagtgctc cagcagaatc cctcagtgtt
     4681 ggagccagca gctgtgggtg gggaagcagc ctccaagcca gccggcagca tgaagcctgc
     4741 ctgtccagcc agcacatctc ctctaaactg gctggccgac ctaaccagcg ggaatgtcaa
     4801 caaggaaaac aaggaaaaac aaccaacaat gccaatttta aagaatgaaa tcaaatgcct
     4861 tccacccctc ccacctttaa gcaaatccag cacagtcctc catacgttta acagcacaat
     4921 tttgacaccc gtaagcaaca acaattctgg tttcctccgg aatctcttga attcttctac
     4981 aggaaagaca gaaaatggac tcaagaatac accaaaaatc cttgatgaca tctttgcctc
     5041 tttggtgcaa aataagacga cttctgattt atctaagagg cctcaaggac taaccatcaa
     5101 gcccagcatt ctgggctttg acactcctca ctattggctt tgtgataatc gcttgctgtg
     5161 cttgcaagac cccaacaata agagcaactg gaatgtgttt agggagtgct ggaaacaagg
     5221 gcagccagtg atggtgtctg gagtgcatca taaattgaac tctgaacttt ggaaacctga
     5281 atccttcagg aaagagtttg gtgagcagga agtagaccta gttaattgta ggaccaatga
     5341 aatcatcaca ggagccacag taggagactt ctgggatgga tttgaagatg ttccaaatcg
     5401 tttgaaaaat gaaaaagaac caatggtgtt gaaacttaag gactggccac caggagaaga
     5461 ttttagagat atgatgcctt ccaggtttga tgatctgatg gccaacattc cactgcccga
     5521 gtacacaagg cgagatggca aactgaattt ggcctctagg ctgccaaact actttgttcg
     5581 gccagatctg ggccccaaga tgtataatgc ttatggatta atcactcctg aagatcggaa
     5641 atatggaaca acaaatcttc acttagatgt atctgatgca gctaatgtca tggtctatgt
     5701 gggaattccc aaaggacagt gtgagcaaga agaagaagtc cttaagacca tccaagatgg
     5761 agattctgac gaactcacaa taaagcgatt tattgaagga aaagagaagc caggagcact
     5821 gtggcacata tatgctgcaa aggacacgga gaagataagg gaatttctta aaaaggtatc
     5881 agaagagcaa ggtcaagaaa acccagcaga ccacgatcct attcatgatc aaagctggta
     5941 tttagaccga tcattaagaa aacgtcttca tcaagagtat ggagttcaag gctgggctat
     6001 tgtacagttt cttggggatg tggtgtttat cccggcagga gctccacatc aggttcataa
     6061 cttatatagc tgcatcaaag tggctgaaga ttttgtttct ccagagcatg ttaaacactg
     6121 cttctggctt actcaggaat tccgatatct gtcacagact cataccaatc acgaagataa
     6181 attacaggtg aagaatgtta tctaccatgc agtgaaagat gcagttgcta tgctgaaagc
     6241 cagtgaatcc agttttggca aacctctcga ggaacaaaaa ctaatatcag aggaagattt
     6301 ggaacagaaa ctaatcagtg aagaagattt agagcaaaag ttaatttccg aggaggactt
     6361 ataggatccg cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg
     6421 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta
     6481 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     6541 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     6601 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc
     6661 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc
     6721 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc
     6781 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg
     6841 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag
     6901 atccaagctg tgaccggcgc ctacgctaga cgccaccatg gtgagcaagg gcgaggagga
     6961 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     7021 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     7081 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     7141 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     7201 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     7261 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     7321 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     7381 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     7441 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     7501 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     7561 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     7621 cggcggcatg gacgagctgt acaagggatc tcgcgagggc agaggaagtc ttctaacatg
     7681 cggtgacgtg gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc
     7741 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     7801 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     7861 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     7921 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     7981 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     8041 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     8101 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     8161 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     8221 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     8281 gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag
     8341 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     8401 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     8461 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     8521 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     8581 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     8641 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     8701 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     8761 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     8821 gccggctctg cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct
     8881 ttggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct
     8941 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata
     9001 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     9061 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     9121 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     9181 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     9241 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     9301 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     9361 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     9421 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     9481 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     9541 tttggaggcc tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt
     9601 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     9661 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     9721 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     9781 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     9841 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     9901 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     9961 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    10021 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    10081 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    10141 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    10201 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    10261 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    10321 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    10381 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
    10441 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    10501 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    10561 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10621 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10681 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10741 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10801 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10861 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10921 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10981 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    11041 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    11101 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    11161 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    11221 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    11281 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    11341 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    11401 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    11461 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    11521 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    11581 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    11641 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    11701 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
    11761 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
    11821 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
    11881 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    11941 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
    12001 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
    12061 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    12121 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    12181 cgacgttgta aaacgacggc cagtgccaag ctg
//
