LOCUS V040577 12186 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V040577 VERSION V040577 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1798..1915 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2005..2208 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2297..6373 /label="JCAD(NM_020848)" /note="JCAD(NM_020848)" /gene="JCAD" CDS 6395..7114 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" promoter 7156..7367 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 7380..7648 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 7675..8274 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 8281..8869 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 8943..9176 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 9248..9369 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 9388..9523 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(9561..9577) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 9585..9601 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(9609..9639) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 9654..9675 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(9963..10551) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(10722..11582) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(11583..11687) /gene="bla" /label="AmpR promoter" primer_bind 12161..12177 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc gccaccatgt acagtgtaga agacctcctg atctctcatg gatacaagct 2341 gtcaagagac cccccagcat cacgcgagga taaccccaag gggcgccagg cagcgaggac 2401 tgggacacga gcaggccagg gcctgcagaa cgggcatgag gatggccctg cggccctcgc 2461 acatcgtaag acgtccgcgg ggaaaggaca tgtgagtgac tccgaaagcc gccgcagcac 2521 accgagaggc cacggggagc cccagagcac ttctgcttcc agaacctcgg aggcggggtt 2581 ttgtaatcaa cccccctcag catggtcctc tcatcccccg actggtaacg accaagccta 2641 ccggagaaga ggacggcaag aagccaggag ccagaagccg agggagcacg aaaacctgga 2701 ggccagagga atggcccaag cccacagcct gcctgtccac gtgagggagg gtccatggga 2761 agttggagga aggtcagagc atgtgatgaa gaagccagtt tgggaagaag aattgcgaat 2821 gtcaggtcct gccaagtggc agaacgtcag cctggaaagc tggaaccagc caaggaaatt 2881 agggaggcag atgtctgatg gagatgggga gagactgttt caagacctgt acccattcat 2941 tcaaggagaa catgtgttga attctcaaaa caaagggaag tctcgctcac tgcctagagt 3001 tctttccccc gagagcctga gttgcacgga aattcccatt ccattaaatg aaagacattc 3061 acctaaaatg ccaccgtatc ctcccacttg cgcaccaaat ttggactcca cgaggaattc 3121 tgagaagagt ggctgctcag ccccatttcc ccggcctaag tttgggaggc ccctcaagcc 3181 cccatcttac agctcgcacc agcagtctag gggaggagcg gacagcagtg actctcagga 3241 cagccagcag atggacgcct atgtccccag gcatgagctc tgcctgtcag accctggatt 3301 ggaacctcca gtgtacgtgc ctccgccctc atacagatcg cccccgcaga acatcccaaa 3361 cccctacttg gaagacacgg tgcccataaa tgtgtgtggc ggtcacagtc aacagcagtc 3421 tccgaccgag aaggctgggg ccagcggtca gcctccttca ggcccccctg gaactgggaa 3481 tgagtatggt gtgagccccc gcttgcctca ggggctcccc gcacatcccc gacctgtcac 3541 tgcctatgac ggcttcgttc agtacattcc ctttgatgat ccacggttac gacattttaa 3601 actagctcag ccccagggtt tctgtgaaga cataaagctt gacgataaat catataactc 3661 cagtcctgtc actgctcaag agccggctca tggaggaatg cagcctgatg gtgccatttg 3721 gaatccacag agcttaatac ccccgtcggg ggatgagaga ggcctggtct tggccgattc 3781 cagcccccgg tggctgtggg gccagccccc cggggatggg gaaaacagtg gcctccccaa 3841 ccagagagac cgctgtgtgg caaggggaca gtggcctgat gtgagaggca gccagcacgg 3901 gcacactgga agacaagttt cctcccctta ctcacagggc gagagcacct gcgaaactca 3961 aaccaagctc aaaaagttcc aaactgggac tcggaccaag aaaagttcaa agaaaaaaat 4021 gaacgagact atattttgct tggtttctat cccagtgaaa tcagaatcac atctgccaga 4081 tagagatatg gacaacaatg acttaaagcc cagtgctgat caaaagaatg ggtctgataa 4141 gagcccggct ctgcaagaac agagtctgct gagcatgtct tccaccgacc tggagctgca 4201 ggccctcaca ggaagcatgg gtgggagaac ggagttccaa aaacaagatc taggggaacc 4261 agaagaagac agacaaacaa atgacctcag tttcatccac cttacaaagc acagagaact 4321 caagcattct ggctcttggc cagggcaccg gtacagagat cagcaaacac aaaccagttt 4381 ctccgaggag ccccaaagtt cgcagctgct ccctggtgca aagctgggag ggccgagtcg 4441 tgcagcattg agtccaaaat gttcagaccc tgctgcctcc gaagctcaga cgcacacagc 4501 attccctacc ggtgatcaca aacagaggcc aagtgcccgt aacctgaaag gtcacaggtc 4561 cctcagccca tccagcaaca gtgcgttctc aaggacttcc ttgtccgtgg accaggcacc 4621 gacgccaaaa gcaggccgaa gtcagccctg cgtggatgtc cacgggcttg gagcccaccc 4681 tgggcctaag cgggaggtgg tgaaggggga gcccacgggc ccttgcaaca gtaaacaact 4741 ctttgggcag tttctcctga aaccggtcag ccgtcgtccc tgggatttga tcagtcagtt 4801 agaaagtttt aacaaggagc tccaggaaga ggaagaaagc agcagtagca gcagcagcag 4861 cagcagcagc agtgaggaga gtgaggcgga gccgcagcag gagaaccgtg ctcactgcag 4921 acaggaggat gtgggcttcc gcggaaacag cccggaaatg agggttgagc cacagccgag 4981 gatgtgggtg ccggagagcc ctgtgtgtag gtcgggaaga ggtgagagta agtctgagag 5041 ctggagtgag gagctgcagc ctggccaccc acgtgcctgg cctccatccc cgggccgctt 5101 tcgcgtggaa gaaggtggcg gtgcaccttt ctgctcagca gatggaagca cgagtgcaga 5161 gaagagacac ctggaggtta gcaacggaat ggacgagctg gcaggtagcc catttcctgt 5221 gacgagaatg tcttcaagat caagtgacgc aaaaccactg cccgcgtcct atccagctga 5281 acctagggag ccccaggaaa gtccgaaaat caccagtgct ttcagctctg tgaaaccaag 5341 tgaagcggtc cctcggaagt ttgacagtgg tggagagagg ggggcagggc tcccactgtc 5401 cctgtctaac aagaaccgag ggctctcagc tccagactta cggtctgtgg ggctcacccc 5461 tgggcaagaa cagggtgcca gtgagctaga ggggtctttg ggtgaagcaa gcacaataga 5521 aatcccccca ggtgagtcct tgcaagccag ggctgcaagg atcctgggca ttgaggtggc 5581 ggtggagtcc ctcctgccgg gcatccggag agcgggacag aaccagcctg ctgagcccga 5641 tgcaagtgcc tgcaccccag agtcccccca ggaagagttg ctatctcgcc cagcaccggc 5701 agatgtcccc agggtgtcca ctgatgcctt ttatggcagg aggaagtgcg gctggaccaa 5761 gagccctctc tttgtagggg acagggacag tgccaggcgg gctcctcagg cttttgagca 5821 ctcagatgtg gacggggttg tcaccagcac agaccctgtc cctgagcctg agcccagccc 5881 cttggagtcc aagttcttcg aacaaaagga tgtggaaaca aaaccaccct tcaggtccac 5941 tttattccat tttgtagaaa gaaccccaag tgtggcaggc tctgaaaaga gacttagaag 6001 cccttccaaa gtgattgaaa gtttacaaga gaaactggcc tccccgccta ggagagcaga 6061 ccctgaccgc ctgatgagaa tgaaagaggt gagctcagtg tcacggatga gagtcctgag 6121 cttcaggaat gccgactccc aggaggacgc cgaggaattg aaggccacca caaggggcca 6181 ggccgggctc ccgggaggcc ttgtgtctcc tggcagtggg gaccgtgccc agagattggg 6241 ccactcactc tctgtgtcca aggacagcat ctccagggaa gagaaggagc atccggcagc 6301 acaaaaggag aagagcatgg atcaagactt ctggtgccca gattcctatg accctagcag 6361 agtggagagg gtgggatccg gaggaggggg aagcatggtg agcaagggcg aggagctgtt 6421 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag 6481 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg 6541 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt 6601 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 6661 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 6721 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 6781 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca 6841 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg 6901 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat 6961 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag 7021 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg 7081 gatcactctc ggcatggacg agctgtacaa gtaagcggcc gcgaaggatc tgcgatcgct 7141 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 7201 gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 7261 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 7321 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag 7381 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt 7441 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg 7501 taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta 7561 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt 7621 cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagatgacc 7681 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc 7741 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac 7801 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 7861 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 7921 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 7981 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 8041 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 8101 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 8161 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 8221 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagtcgac 8281 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 8341 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 8401 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 8461 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 8521 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 8581 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 8641 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc 8701 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 8761 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt 8821 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt 8881 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 8941 actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc 9001 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 9061 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 9121 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 9181 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 9241 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 9301 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 9361 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat 9421 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 9481 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac 9541 ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 9601 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 9661 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 9721 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 9781 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 9841 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 9901 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 9961 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 10021 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 10081 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 10141 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 10201 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 10261 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 10321 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 10381 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 10441 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 10501 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 10561 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 10621 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 10681 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 10741 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 10801 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 10861 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 10921 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 10981 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 11041 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 11101 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 11161 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 11221 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 11281 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 11341 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 11401 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 11461 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 11521 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 11581 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 11641 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 11701 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg 11761 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 11821 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 11881 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca 11941 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg 12001 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 12061 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 12121 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc 12181 aagctg //