LOCUS       V047305                11833 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047305
VERSION     V047305
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5859
                     /label="ITGA2(NM_002203)"
                     /note="ITGA2(NM_002203)"
                     /gene="ITGA2"
     CDS             5866..5931
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5975..6186
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6199..6467
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6496..6505
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6526..7188
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7258..7311
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7312..7911
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7912..8500
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8574..8807
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8879..9000
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9040..9175
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9208..9224)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9232..9248
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9256..9286)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9301..9322
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9610..10198)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10369..11229)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11230..11334)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11808..11824
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ggccagaacg gacaggggcc
     2341 gcgccgctgc cgctgctgct ggtgttagcg ctcagtcaag gcattttaaa ttgttgtttg
     2401 gcctacaatg ttggtctccc agaagcaaaa atattttccg gtccttcaag tgaacagttt
     2461 ggctatgcag tgcagcagtt tataaatcca aaaggcaact ggttactggt tggttcaccc
     2521 tggagtggct ttcctgagaa ccgaatggga gatgtgtata aatgtcctgt tgacctatcc
     2581 actgccacat gtgaaaaact aaatttgcaa acttcaacaa gcattccaaa tgttactgag
     2641 atgaaaacca acatgagcct cggcttgatc ctcaccagga acatgggaac tggaggtttt
     2701 ctcacatgtg gtcctctgtg ggcacagcaa tgtgggaatc agtattacac aacgggtgtg
     2761 tgttctgaca tcagtcctga ttttcagctc tcagccagct tctcacctgc aactcagccc
     2821 tgcccttccc tcatagatgt tgtggttgtg tgtgatgaat caaatagtat ttatccttgg
     2881 gatgcagtaa agaatttttt ggaaaaattt gtacaaggcc tggatatagg ccccacaaag
     2941 acacaggtgg ggttaattca gtatgccaat aatccaagag ttgtgtttaa cttgaacaca
     3001 tataaaacca aagaagaaat gattgtagca acatcccaga catcccaata tggtggggac
     3061 ctcacaaaca cattcggagc aattcaatat gcaagaaaat atgcttattc agcagcttct
     3121 ggtgggcgac gaagtgctac gaaagtaatg gtagttgtaa ctgacggtga atcacatgat
     3181 ggttcaatgt tgaaagctgt gattgatcaa tgcaaccatg acaatatact gaggtttggc
     3241 atagcagttc ttgggtactt aaacagaaac gcccttgata ctaaaaattt aataaaagaa
     3301 ataaaagcaa tcgctagtat tccaacagaa agatactttt tcaatgtgtc tgatgaagca
     3361 gctctactag aaaaggctgg gacattagga gaacaaattt tcagcattga aggtactgtt
     3421 caaggaggag acaactttca gatggaaatg tcacaagtgg gattcagtgc agattactct
     3481 tctcaaaatg atattctgat gctgggtgca gtgggagctt ttggctggag tgggaccatt
     3541 gtccagaaga catctcatgg ccatttgatc tttcctaaac aagcctttga ccaaattctg
     3601 caggacagaa atcacagttc atatttaggt tactctgtgg ctgcaatttc tactggagaa
     3661 agcactcact ttgttgctgg tgctcctcgg gcaaattata ccggccagat agtgctatat
     3721 agtgtgaatg agaatggcaa tatcacggtt attcaggctc accgaggtga ccagattggc
     3781 tcctattttg gtagtgtgct gtgttcagtt gatgtggata aagacaccat tacagacgtg
     3841 ctcttggtag gtgcaccaat gtacatgagt gacctaaaga aagaggaagg aagagtctac
     3901 ctgtttacta tcaaagaggg cattttgggt cagcaccaat ttcttgaagg ccccgagggc
     3961 attgaaaaca ctcgatttgg ttcagcaatt gcagctcttt cagacatcaa catggatggc
     4021 tttaatgatg tgattgttgg ttcaccacta gaaaatcaga attctggagc tgtatacatt
     4081 tacaatggtc atcagggcac tatccgcaca aagtattccc agaaaatctt gggatccgat
     4141 ggagccttta ggagccatct ccagtacttt gggaggtcct tggatggcta tggagattta
     4201 aatggggatt ccatcaccga tgtgtctatt ggtgcctttg gacaagtggt tcaactctgg
     4261 tcacaaagta ttgctgatgt agctatagaa gcttcattca caccagaaaa aatcactttg
     4321 gtcaacaaga atgctcagat aattctcaaa ctctgcttca gtgcaaagtt cagacctact
     4381 aagcaaaaca atcaagtggc cattgtatat aacatcacac ttgatgcaga tggattttca
     4441 tccagagtaa cctccagggg gttatttaaa gaaaacaatg aaaggtgcct gcagaagaat
     4501 atggtagtaa atcaagcaca gagttgcccc gagcacatca tttatataca ggagccctct
     4561 gatgttgtca actctttgga tttgcgtgtg gacatcagtc tggaaaaccc tggcactagc
     4621 cctgcccttg aagcctattc tgagactgcc aaggtcttca gtattccttt ccacaaagac
     4681 tgtggtgagg acggactttg catttctgat ctagtcctag atgtccgaca aataccagct
     4741 gctcaagaac aaccctttat tgtcagcaac caaaacaaaa ggttaacatt ttcagtaacg
     4801 ctgaaaaata aaagggaaag tgcatacaac actggaattg ttgttgattt ttcagaaaac
     4861 ttgttttttg catcattctc cctgccggtt gatgggacag aagtaacatg ccaggtggct
     4921 gcatctcaga agtctgttgc ctgcgatgta ggctaccctg ctttaaagag agaacaacag
     4981 gtgactttta ctattaactt tgacttcaat cttcaaaacc ttcagaatca ggcgtctctc
     5041 agtttccaag ccttaagtga aagccaagaa gaaaacaagg ctgataattt ggtcaacctc
     5101 aaaattcctc tcctgtatga tgctgaaatt cacttaacaa gatctaccaa cataaatttt
     5161 tatgaaatct cttcggatgg gaatgttcct tcaatcgtgc acagttttga agatgttggt
     5221 ccaaaattca tcttctccct gaaggtaaca acaggaagtg ttccagtaag catggcaact
     5281 gtaatcatcc acatccctca gtataccaaa gaaaagaacc cactgatgta cctaactggg
     5341 gtgcaaacag acaaggctgg tgacatcagt tgtaatgcag atatcaatcc actgaaaata
     5401 ggacaaacat cttcttctgt atctttcaaa agtgaaaatt tcaggcacac caaagaattg
     5461 aactgcagaa ctgcttcctg tagtaatgtt acctgctggt tgaaagacgt tcacatgaaa
     5521 ggagaatact ttgttaatgt gactaccaga atttggaacg ggactttcgc atcatcaacg
     5581 ttccagacag tacagctaac ggcagctgca gaaatcaaca cctataaccc tgagatatat
     5641 gtgattgaag ataacactgt tacgattccc ctgatgataa tgaaacctga tgagaaagcc
     5701 gaagtaccaa caggagttat aataggaagt ataattgctg gaatcctttt gctgttagct
     5761 ctggttgcaa ttttatggaa gctcggcttc ttcaaaagaa aatatgaaaa gatgaccaaa
     5821 aatccagatg agattgatga gaccacagag ctcagtagcg gatccgacta caaagaccat
     5881 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagcggccg
     5941 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     6001 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     6061 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     6121 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     6181 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     6241 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     6301 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     6361 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     6421 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     6481 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc
     6541 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag
     6601 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc
     6661 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac
     6721 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc
     6781 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag
     6841 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg
     6901 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc
     6961 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac
     7021 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag
     7081 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg
     7141 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct
     7201 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag
     7261 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag
     7321 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     7381 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     7441 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     7501 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     7561 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     7621 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     7681 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     7741 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     7801 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     7861 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct
     7921 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     7981 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     8041 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     8101 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     8161 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     8221 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     8281 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     8341 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     8401 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     8461 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat
     8521 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     8581 gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt
     8641 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8701 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8761 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     8821 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     8881 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8941 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9001 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     9061 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9121 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
     9181 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     9241 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9301 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9361 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9421 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9481 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9541 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9601 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9661 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9721 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9781 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9841 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9901 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9961 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10021 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    10081 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10141 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10201 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10261 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10321 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10381 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10441 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10501 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10561 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10621 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10681 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10741 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10801 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10861 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10921 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10981 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11041 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11101 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11161 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11221 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11281 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11341 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    11401 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    11461 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    11521 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    11581 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    11641 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    11701 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    11761 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    11821 cagtgccaag ctg
//
