LOCUS V030744 9344 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030744 VERSION V030744 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..3345 /label="HOMER2(NM_004839)" /note="HOMER2(NM_004839)" /gene="HOMER2" CDS 3352..3378 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3382..3408 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3412..3438 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 3503..3714 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3727..3995 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 4024..4033 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 4054..4716 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 4786..4839 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 4840..5439 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5440..6027 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6101..6334 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6406..6527 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6546..6681 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6719..6735) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6743..6759 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6767..6797) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6812..6833 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7121..7709) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7880..8740) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8741..8845) /gene="bla" /label="AmpR promoter" primer_bind 9319..9335 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gagaacagcc catcttcacc 2341 acccgagcgc atgtcttcca gattgacccc aacaccaaga agaactggat gcctgcgagc 2401 aagcaggcgg tcaccgtttc ctacttctat gatgtcacaa ggaacagcta tcggatcatc 2461 agtgtggacg gagccaaggt gatcataaac agcacaatca caccgaatat gaccttcacc 2521 aaaacgtcac agaagtttgg gcagtgggcc gacagcagag ccaacacagt gtttggtttg 2581 gggttttcct ctgagcagca gctgacaaag tttgcagaga aattccagga ggtgaaagaa 2641 gctgccaaga tagccaaaga caagacgcag gagaaaatcg agacctcaag taatcattcc 2701 caagcatcca gtgtcaacgg gacggacgat gaaaaggcct ctcacgccgg tccagccaac 2761 acacacctga agtctgagaa tgacaagctg aagattgcct tgacgcagag cgcagccaac 2821 gtgaagaagt gggagatcga gctgcagacc cttcgggaga gcaatgcacg gctgaccaca 2881 gcactgcagg agtcggcagc cagtgtggag cagtggaaga ggcagttctc catctgccgt 2941 gatgagaatg accggctccg caacaagatt gatgagctgg aagaacaatg cagtgagatc 3001 aacagagaga aggagaagaa cacgcagctg aagaggagga tcgaggagct ggaggcagag 3061 ctccgagaaa aggagacaga gctgaaagat ctccgaaaac aaagtgaaat catacctcag 3121 ctcatgtcag agtgcgaata tgtctctgag aagctagagg cggcagagag agacaatcaa 3181 aacctggaag acaaagtgcg ttccttaaag acagacattg aggagagcaa ataccgacag 3241 cgccacctga aggtggagtt gaagagcttc ctggaggtgc tggacgggaa gattgacgac 3301 ctgcatgact tccgccgagg gctctccaag ctgggcaccg ataacctcga gtacccatac 3361 gacgtacctg attatgcaac ttatccttat gatgtacctg attatgctag ctatccttat 3421 gatgtcccag actacgcata gggatccctg cgatatccag cgcggccgcg aaggatctgc 3481 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg 3541 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa 3601 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt 3661 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag 3721 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca 3781 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc 3841 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc 3901 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc 3961 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta 4021 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc 4081 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag 4141 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac 4201 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac 4261 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag 4321 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg 4381 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac 4441 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg 4501 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg 4561 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc 4621 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag 4681 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat 4741 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt 4801 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg 4861 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 4921 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 4981 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 5041 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 5101 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 5161 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 5221 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 5281 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 5341 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 5401 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa 5461 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 5521 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 5581 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 5641 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 5701 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 5761 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 5821 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt 5881 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 5941 cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt 6001 cggatctccc tttggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca 6061 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 6121 caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga 6181 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 6241 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 6301 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 6361 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca 6421 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 6481 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc 6541 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 6601 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 6661 gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt 6721 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 6781 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 6841 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 6901 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 6961 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 7021 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 7081 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 7141 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 7201 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 7261 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 7321 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 7381 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 7441 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 7501 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 7561 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 7621 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 7681 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 7741 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 7801 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 7861 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 7921 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 7981 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 8041 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 8101 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 8161 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 8221 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 8281 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 8341 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 8401 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 8461 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 8521 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 8581 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 8641 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 8701 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 8761 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 8821 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag 8881 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 8941 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 9001 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 9061 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 9121 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 9181 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 9241 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 9301 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg //