LOCUS V032368 13090 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V032368 VERSION V032368 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2290..5508 /label="GUCY2C(NM_004963)" /note="GUCY2C(NM_004963)" /gene="GUCY2C" CDS 5536..7188 /codon_start=1 /note="synthetic luc2 version of the luciferase gene" /product="firefly luciferase" /protein_id="" /transl_table=1 /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*" /label="luciferase" promoter 7249..7460 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 7473..7741 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 7770..7779 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 7800..8462 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 8532..8585 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 8586..9185 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 9186..9773 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 9847..10080 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 10152..10273 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 10292..10427 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(10465..10481) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 10489..10505 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(10513..10543) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 10558..10579 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(10867..11455) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(11626..12486) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(12487..12591) /gene="bla" /label="AmpR promoter" primer_bind 13065..13081 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctaga tgaagacgtt gctgttggac ttggctttgt ggtcactgct cttccagccc 2341 gggtggctgt cctttagttc ccaggtgagt cagaactgcc acaatggcag ctatgaaatc 2401 agcgtcctga tgatgggcaa ctcagccttt gcagagcccc tgaaaaactt ggaagatgcg 2461 gtgaatgagg ggctggaaat agtgagagga cgtctgcaaa atgctggcct aaatgtgact 2521 gtgaacgcta ctttcatgta ttcggatggt ctgattcata actcaggcga ctgccggagt 2581 agcacctgtg aaggcctcga cctactcagg aaaatttcaa atgcacaacg gatgggctgt 2641 gtcctcatag ggccctcatg tacatactcc accttccaga tgtaccttga cacagaattg 2701 agctacccca tgatctcagc tggaagtttt ggattgtcat gtgactataa agaaacctta 2761 accaggctga tgtctccagc tagaaagttg atgtacttct tggttaactt ttggaaaacc 2821 aacgatctgc ccttcaaaac ttattcctgg agcacttcgt atgtttacaa gaatggtaca 2881 gaaactgagg actgtttctg gtaccttaat gctctggagg ctagcgtttc ctatttctcc 2941 cacgaactcg gctttaaggt ggtgttaaga caagataagg agtttcagga tatcttaatg 3001 gaccacaaca ggaaaagcaa tgtgattatt atgtgtggtg gtccagagtt cctctacaag 3061 ctgaagggtg accgagcagt ggctgaagac attgtcatta ttctagtgga tcttttcaat 3121 gaccagtact ttgaggacaa tgtcacagcc cctgactata tgaaaaatgt ccttgttctg 3181 acgctgtctc ctgggaattc ccttctaaat agctctttct ccaggaatct atcaccaaca 3241 aaacgagact ttgctcttgc ctatttgaat ggaatcctgc tctttggaca tatgctgaag 3301 atatttcttg aaaatggaga aaatattacc acccccaaat ttgctcatgc tttcaggaat 3361 ctcacttttg aagggtatga cggtccagtg accttggatg actgggggga tgttgacagt 3421 accatggtgc ttctgtatac ctctgtggac accaagaaat acaaggttct tttgacctat 3481 gatacccacg taaataagac ctatcctgtg gatatgagcc ccacattcac ttggaagaac 3541 tctaaacttc ctaatgatat tacaggccgg ggccctcaga tcctgatgat tgcagtcttc 3601 accctcactg gagctgtggt gctgctcctg ctcgtcgctc tcctgatgct cagaaaatat 3661 agaaaagatt atgaacttcg tcagaaaaaa tggtcccaca ttcctcctga aaatatcttt 3721 cctctggaga ccaatgagac caatcatgtt agcctcaaga tcgatgatga caaaagacga 3781 gatacaatcc agagactacg acagtgcaaa tacgacaaaa agcgagtgat tctcaaagat 3841 ctcaagcaca atgatggtaa tttcactgaa aaacagaaga tagaattgaa caagttgctt 3901 cagattgact attacaacct gaccaagttc tacggcacag tgaaacttga taccatgatc 3961 ttcggggtga tagaatactg tgagagagga tccctccggg aagttttaaa tgacacaatt 4021 tcctaccctg atggcacatt catggattgg gagtttaaga tctctgtctt gtatgacatt 4081 gctaagggaa tgtcatatct gcactccagt aagacagaag tccatggtcg tctgaaatct 4141 accaactgcg tagtggacag tagaatggtg gtgaagatca ctgattttgg ctgcaattcc 4201 attttacctc caaaaaagga cctgtggaca gctccagagc acctccgcca agccaacatc 4261 tctcagaaag gagatgtgta cagctatggg atcatcgcac aggagatcat cctgcggaaa 4321 gaaaccttct acactttgag ctgtcgggac cggaatgaga agattttcag agtggaaaat 4381 tccaatggaa tgaaaccctt ccgcccagat ttattcttgg aaacagcaga ggaaaaagag 4441 ctagaagtgt acctacttgt aaaaaactgt tgggaggaag atccagaaaa gagaccagat 4501 ttcaaaaaaa ttgagactac acttgccaag atatttggac tttttcatga ccaaaaaaat 4561 gaaagctata tggatacctt gatccgacgt ctacagctat attctcgaaa cctggaacat 4621 ctggtagagg aaaggacaca gctgtacaag gcagagaggg acagggctga cagacttaac 4681 tttatgttgc ttccaaggct agtggtaaag tctctgaagg agaaaggctt tgtggagccg 4741 gaactatatg aggaagttac aatctacttc agtgacattg taggtttcac tactatctgc 4801 aaatacagca cccccatgga agtggtggac atgcttaatg acatctataa gagttttgac 4861 cacattgttg atcatcatga tgtctacaag gtggaaacca tcggtgatgc gtacatggtg 4921 gctagtggtt tgcctaagag aaatggcaat cggcatgcaa tagacattgc caagatggcc 4981 ttggaaatcc tcagcttcat ggggaccttt gagctggagc atcttcctgg cctcccaata 5041 tggattcgca ttggagttca ctctggtccc tgtgctgctg gagttgtggg aatcaagatg 5101 cctcgttatt gtctatttgg agatacggtc aacacagcct ctaggatgga atccactggc 5161 ctccctttga gaattcacgt gagtggctcc accatagcca tcctgaagag aactgagtgc 5221 cagttccttt atgaagtgag aggagaaaca tacttaaagg gaagaggaaa tgagactacc 5281 tactggctga ctgggatgaa ggaccagaaa ttcaacctgc caacccctcc tactgtggag 5341 aatcaacagc gtttgcaagc agaattttca gacatgattg ccaactcttt acagaaaaga 5401 caggcagcag ggataagaag ccaaaaaccc agacgggtag ccagctataa aaaaggcact 5461 ctggaatact tgcagctgaa taccacagac aaggagagca cctattttcc tgatacgaac 5521 tcggaattcg ccaccatgga agatgccaaa aacattaaga agggcccagc gccattctac 5581 ccactcgaag acgggaccgc cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg 5641 gtgcccggca ccatcgcctt taccgacgca catatcgagg tggacattac ctacgccgag 5701 tacttcgaga tgagcgttcg gctggcagaa gctatgaagc gctatgggct gaatacaaac 5761 catcggatcg tggtgtgcag cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc 5821 ctgttcatcg gtgtggctgt ggccccagct aacgacatct acaacgagcg cgagctgctg 5881 aacagcatgg gcatcagcca gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag 5941 atcctcaacg tgcaaaagaa gctaccgatc atacaaaaga tcatcatcat ggatagcaag 6001 accgactacc agggcttcca aagcatgtac accttcgtga cttcccattt gccacccggc 6061 ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg acaaaaccat cgccctgatc 6121 atgaacagta gtggcagtac cggattgccc aagggcgtag ccctaccgca ccgcaccgct 6181 tgtgtccgat tcagtcatgc ccgcgacccc atcttcggca accagatcat ccccgacacc 6241 gctatcctca gcgtggtgcc atttcaccac ggcttcggca tgttcaccac gctgggctac 6301 ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg aggaggagct attcttgcgc 6361 agcttgcaag actataagat tcaatctgcc ctgctggtgc ccacactatt tagcttcttc 6421 gctaagagca ctctcatcga caagtacgac ctaagcaact tgcacgagat cgccagcggc 6481 ggggcgccgc tcagcaagga ggtaggtgag gccgtggcca aacgcttcca cctaccaggc 6541 atccgccagg gctacggcct gacagaaaca accagcgcca ttctgatcac ccccgaaggg 6601 gacgacaagc ctggcgcagt aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac 6661 ttggacaccg gtaagacact gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc 6721 atgatcatga gcggctacgt taacaacccc gaggctacaa acgctctcat cgacaaggac 6781 ggctggctgc acagcggcga catcgcctac tgggacgagg acgagcactt cttcatcgtg 6841 gaccggctga agagcctgat caaatacaag ggctaccagg tagccccagc cgaactggag 6901 agcatcctgc tgcaacaccc caacatcttc gacgccgggg tcgccggcct gcccgacgac 6961 gatgccggcg agctgcccgc cgcagtcgtc gtgctggaac acggtaaaac catgaccgag 7021 aaggagatcg tggactatgt ggccagccag gttacaaccg ccaagaagct gcgcggtggt 7081 gttgtgttcg tggacgaggt gcctaaagga ctgaccggca agttggacgc ccgcaagatc 7141 cgcgagattc tcattaaggc caagaagggc ggcaagatcg ccgtgtagga tccctgcgat 7201 atccagcgcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca 7261 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga 7321 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 7381 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 7441 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc 7501 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct 7561 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg 7621 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc 7681 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat 7741 ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag agcggcctgc 7801 ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg 7861 tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca 7921 aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc 7981 acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg 8041 gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca 8101 gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc 8161 ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc 8221 tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg 8281 acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc 8341 ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca 8401 gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg 8461 ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca 8521 ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg 8581 gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg 8641 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc 8701 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 8761 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 8821 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 8881 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 8941 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 9001 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 9061 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 9121 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg 9181 cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 9241 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 9301 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 9361 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 9421 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 9481 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 9541 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc 9601 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 9661 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 9721 gtctccgcct tcgccctcag acgagtcgga tctccctttg gccgcctccc cgcctggtac 9781 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 9841 ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg 9901 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 9961 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 10021 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 10081 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 10141 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 10201 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 10261 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc 10321 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 10381 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag 10441 agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 10501 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 10561 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 10621 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 10681 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 10741 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 10801 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 10861 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 10921 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 10981 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 11041 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 11101 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 11161 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 11221 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 11281 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 11341 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 11401 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 11461 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 11521 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 11581 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 11641 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 11701 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 11761 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 11821 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 11881 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 11941 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 12001 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 12061 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 12121 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 12181 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 12241 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 12301 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 12361 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 12421 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 12481 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 12541 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 12601 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 12661 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 12721 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 12781 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 12841 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 12901 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg 12961 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 13021 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 13081 tgccaagctg //