LOCUS V035220 11105 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V035220 VERSION V035220 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..5106 /label="GLIS3(NM_001042413)" /note="GLIS3(NM_001042413)" /gene="GLIS3" CDS 5113..5139 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 5143..5169 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 5173..5199 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 5264..5475 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 5488..5756 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 5785..5794 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 5815..6477 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 6547..6600 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 6601..7200 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7201..7788 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7862..8095 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 8167..8288 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 8307..8442 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(8480..8496) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8504..8520 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8528..8558) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8573..8594 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8882..9470) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9641..10501) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10502..10606) /gene="bla" /label="AmpR promoter" primer_bind 11080..11096 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga atggaagatc atgcagcatg 2341 agtctccacc ggacatcggg aaccccacag gggcctagga tggtcagtgg tcatcacatt 2401 cctgccatcc gagcccactc cgggactcct ggcccctcgc cctgtggcag cacatcgagt 2461 cccactatgg caagccttgc taacaacctc catctcaaga tgccctcagg aggagggatg 2521 gctcctcaga acaacgtggc tgagagccgc atccatctgc ctgccttaag ccccaggaga 2581 caaatgctca ccaatgggaa gccgcgattc caggtcaccc aggctggagg catgtcaggg 2641 tcacatactt taaagccaaa gcagcaggag tttggaagcc cttttcctcc aaatcctggg 2701 aaaggggctc ttggctttgg gcctcagtgc aagtccattg gaaaaggcag ctgcaacaat 2761 ctagtggtca ccagcagtcc catgatggtt cagcgactgg gactcatttc acctccagca 2821 agccaggtct ctacagcatg caaccagatc agtcctagct tacagagggc aatgaatgca 2881 gccaacctga atatacctcc ttcagatacc aggtccctta tttcgcgtga gtctttggcg 2941 tccacgacct tgagtctgac ggaaagtcag tcggcctcaa gcatgaagca ggagtggtcc 3001 cagggctaca gggccctccc ttcgctctcc aaccacggct ctcagaatgg ccttgatcta 3061 ggggatctcc ttagccttcc tcccgggaca tccatgtcca gcaatagtgt ctctaactca 3121 ttaccatcct acctttttgg cacggaaagt agccactctc cttaccctag tcctcggcac 3181 tcatccacca ggtcccactc ggcccgctcc aagaagagag cgctgtcctt gtccccgctg 3241 tccgatggca tcgggataga tttcaatacc atcatccgca cgtcgcccac gtccttggtg 3301 gcctacatca acgggtcgag ggcttcgccg gccaacctgt ccccgcagcc ggaggtctac 3361 gggcatttcc tgggcgtgcg cggcagctgc attccccagc cgcgcccggt gcccggcagc 3421 cagaagggcg tgctggtggc ccctggaggc ctggcgctgc cggcctacgg cgaggacggg 3481 gccctggagc acgagcgcat gcaacagctg gagcacggcg gcctgcagcc aggcctggtc 3541 aaccacatgg tggtgcagca tggcctgccg ggccccgaca gccagtcggc cggcctgttc 3601 aagaccgaac gcctggagga gttcccgggc agcaccgtag acctaccccc cgcgcctccg 3661 ctccctcctc tgccgccgcc cccaggcccc ccaccccctt accatgccca tgcgcacctt 3721 caccacccgg agctcgggcc ccacgcccag cagctggcct tgccccaggc caccctggac 3781 gacgacgggg agatggacgg catcgggggc aagcattgct gccgctggat cgactgcagc 3841 gccctgtacg accagcagga ggagctcgtg cggcacatcg agaaggtcca catcgaccag 3901 cgcaaagggg aggacttcac ttgcttctgg gccggttgcc ctcgaagata caagcccttc 3961 aacgcccgct ataaactgct gatccacatg agagtccact ctggggagaa gcccaacaag 4021 tgtacgtttg aaggttgcga gaaggccttt tcaaggcttg aaaatctcaa gatccacttg 4081 cggagccaca caggcgagaa gccgtatttg tgccagcatc cgggttgtca gaaggccttc 4141 agtaactcca gtgaccgcgc caaacaccag cggacgcatc tggacaccaa accttatgct 4201 tgtcaaattc caggatgtac caaacgctac acagacccaa gttccctaag aaagcatgtg 4261 aaggcacatt cttccaaaga gcaacaagca aggaaaaagt tgcggtccag cacagagctc 4321 catccagacc tgctcacaga ttgcctcacc gtgcagtccc tgcagccggc cacttcccct 4381 agagatgctg ctgctgaagg gaccgtggga cgctcccctg gacccgggcc tgacctctat 4441 tcagctccca ttttctccag caattattca agccgaagtg gaacagctgc tggggccgta 4501 ccacccccac atcctgtcag tcacccttct ccaggacata atgtacaggg gagccctcac 4561 aacccctcct cccagttacc tccactcaca gctgtggacg caggagctga gaggtttgca 4621 ccttctgctc catctcctca ccacatcagc ccccggagag ttccagctcc ttcttcaata 4681 ctgcaaagaa cacagcctcc ctatacccag cagccatcag gttcacacct gaagtcctat 4741 cagccagaaa caaactcttc ttttcaacca aatggtatcc atgtccatgg attttatggg 4801 cagctgcaga agttctgtcc cccacactac cccgattccc agagaattgt gccgcctgtc 4861 agctcctgca gtgtggtgcc ttcgtttgag gactgcctag tccctacatc catgggccag 4921 gccagttttg atgttttcca cagagccttc tcgactcact cgggcattac agtgtatgat 4981 ttaccttcaa gttcctcgag cctctttggg gagtctctcc gcagcggggc tgaagatgct 5041 accttcttgc agatcagcac cgtggaccgc tgtcctagcc agctctcctc tgtctacacc 5101 gaaggcctcg agtacccata cgacgtacct gattatgcaa cttatcctta tgatgtacct 5161 gattatgcta gctatcctta tgatgtccca gactacgcat agggatccct gcgatatcca 5221 gcgcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 5281 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 5341 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 5401 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 5461 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 5521 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 5581 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 5641 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 5701 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 5761 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 5821 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 5881 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 5941 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 6001 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 6061 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 6121 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 6181 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 6241 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 6301 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 6361 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 6421 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 6481 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 6541 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 6601 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 6661 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 6721 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 6781 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 6841 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 6901 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 6961 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 7021 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 7081 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 7141 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 7201 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 7261 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 7321 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 7381 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 7441 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 7501 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 7561 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc 7621 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 7681 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc 7741 cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta 7801 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 7861 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 7921 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 7981 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 8041 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 8101 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 8161 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 8221 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 8281 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 8341 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 8401 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 8461 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 8521 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 8581 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 8641 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 8701 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 8761 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 8821 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 8881 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 8941 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 9001 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 9061 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 9121 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 9181 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 9241 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 9301 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 9361 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 9421 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 9481 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 9541 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 9601 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 9661 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 9721 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 9781 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 9841 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 9901 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 9961 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 10021 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 10081 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 10141 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 10201 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 10261 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 10321 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 10381 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 10441 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 10501 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 10561 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 10621 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 10681 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 10741 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 10801 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 10861 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 10921 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 10981 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 11041 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 11101 agctg //