LOCUS       V035220                11105 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035220
VERSION     V035220
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5106
                     /label="GLIS3(NM_001042413)"
                     /note="GLIS3(NM_001042413)"
                     /gene="GLIS3"
     CDS             5113..5139
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5143..5169
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5173..5199
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5264..5475
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5488..5756
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5785..5794
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5815..6477
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6547..6600
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6601..7200
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7201..7788
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7862..8095
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8167..8288
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8307..8442
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8480..8496)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8504..8520
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8528..8558)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8573..8594
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8882..9470)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9641..10501)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10502..10606)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11080..11096
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga atggaagatc atgcagcatg
     2341 agtctccacc ggacatcggg aaccccacag gggcctagga tggtcagtgg tcatcacatt
     2401 cctgccatcc gagcccactc cgggactcct ggcccctcgc cctgtggcag cacatcgagt
     2461 cccactatgg caagccttgc taacaacctc catctcaaga tgccctcagg aggagggatg
     2521 gctcctcaga acaacgtggc tgagagccgc atccatctgc ctgccttaag ccccaggaga
     2581 caaatgctca ccaatgggaa gccgcgattc caggtcaccc aggctggagg catgtcaggg
     2641 tcacatactt taaagccaaa gcagcaggag tttggaagcc cttttcctcc aaatcctggg
     2701 aaaggggctc ttggctttgg gcctcagtgc aagtccattg gaaaaggcag ctgcaacaat
     2761 ctagtggtca ccagcagtcc catgatggtt cagcgactgg gactcatttc acctccagca
     2821 agccaggtct ctacagcatg caaccagatc agtcctagct tacagagggc aatgaatgca
     2881 gccaacctga atatacctcc ttcagatacc aggtccctta tttcgcgtga gtctttggcg
     2941 tccacgacct tgagtctgac ggaaagtcag tcggcctcaa gcatgaagca ggagtggtcc
     3001 cagggctaca gggccctccc ttcgctctcc aaccacggct ctcagaatgg ccttgatcta
     3061 ggggatctcc ttagccttcc tcccgggaca tccatgtcca gcaatagtgt ctctaactca
     3121 ttaccatcct acctttttgg cacggaaagt agccactctc cttaccctag tcctcggcac
     3181 tcatccacca ggtcccactc ggcccgctcc aagaagagag cgctgtcctt gtccccgctg
     3241 tccgatggca tcgggataga tttcaatacc atcatccgca cgtcgcccac gtccttggtg
     3301 gcctacatca acgggtcgag ggcttcgccg gccaacctgt ccccgcagcc ggaggtctac
     3361 gggcatttcc tgggcgtgcg cggcagctgc attccccagc cgcgcccggt gcccggcagc
     3421 cagaagggcg tgctggtggc ccctggaggc ctggcgctgc cggcctacgg cgaggacggg
     3481 gccctggagc acgagcgcat gcaacagctg gagcacggcg gcctgcagcc aggcctggtc
     3541 aaccacatgg tggtgcagca tggcctgccg ggccccgaca gccagtcggc cggcctgttc
     3601 aagaccgaac gcctggagga gttcccgggc agcaccgtag acctaccccc cgcgcctccg
     3661 ctccctcctc tgccgccgcc cccaggcccc ccaccccctt accatgccca tgcgcacctt
     3721 caccacccgg agctcgggcc ccacgcccag cagctggcct tgccccaggc caccctggac
     3781 gacgacgggg agatggacgg catcgggggc aagcattgct gccgctggat cgactgcagc
     3841 gccctgtacg accagcagga ggagctcgtg cggcacatcg agaaggtcca catcgaccag
     3901 cgcaaagggg aggacttcac ttgcttctgg gccggttgcc ctcgaagata caagcccttc
     3961 aacgcccgct ataaactgct gatccacatg agagtccact ctggggagaa gcccaacaag
     4021 tgtacgtttg aaggttgcga gaaggccttt tcaaggcttg aaaatctcaa gatccacttg
     4081 cggagccaca caggcgagaa gccgtatttg tgccagcatc cgggttgtca gaaggccttc
     4141 agtaactcca gtgaccgcgc caaacaccag cggacgcatc tggacaccaa accttatgct
     4201 tgtcaaattc caggatgtac caaacgctac acagacccaa gttccctaag aaagcatgtg
     4261 aaggcacatt cttccaaaga gcaacaagca aggaaaaagt tgcggtccag cacagagctc
     4321 catccagacc tgctcacaga ttgcctcacc gtgcagtccc tgcagccggc cacttcccct
     4381 agagatgctg ctgctgaagg gaccgtggga cgctcccctg gacccgggcc tgacctctat
     4441 tcagctccca ttttctccag caattattca agccgaagtg gaacagctgc tggggccgta
     4501 ccacccccac atcctgtcag tcacccttct ccaggacata atgtacaggg gagccctcac
     4561 aacccctcct cccagttacc tccactcaca gctgtggacg caggagctga gaggtttgca
     4621 ccttctgctc catctcctca ccacatcagc ccccggagag ttccagctcc ttcttcaata
     4681 ctgcaaagaa cacagcctcc ctatacccag cagccatcag gttcacacct gaagtcctat
     4741 cagccagaaa caaactcttc ttttcaacca aatggtatcc atgtccatgg attttatggg
     4801 cagctgcaga agttctgtcc cccacactac cccgattccc agagaattgt gccgcctgtc
     4861 agctcctgca gtgtggtgcc ttcgtttgag gactgcctag tccctacatc catgggccag
     4921 gccagttttg atgttttcca cagagccttc tcgactcact cgggcattac agtgtatgat
     4981 ttaccttcaa gttcctcgag cctctttggg gagtctctcc gcagcggggc tgaagatgct
     5041 accttcttgc agatcagcac cgtggaccgc tgtcctagcc agctctcctc tgtctacacc
     5101 gaaggcctcg agtacccata cgacgtacct gattatgcaa cttatcctta tgatgtacct
     5161 gattatgcta gctatcctta tgatgtccca gactacgcat agggatccct gcgatatcca
     5221 gcgcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     5281 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     5341 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     5401 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     5461 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     5521 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     5581 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     5641 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     5701 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     5761 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc
     5821 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc
     5881 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc
     5941 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc
     6001 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac
     6061 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac
     6121 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag
     6181 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac
     6241 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc
     6301 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc
     6361 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac
     6421 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga
     6481 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga
     6541 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
     6601 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     6661 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     6721 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     6781 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     6841 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     6901 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     6961 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     7021 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     7081 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     7141 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     7201 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7261 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7321 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7381 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7441 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7501 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7561 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     7621 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7681 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc
     7741 cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta
     7801 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     7861 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct
     7921 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7981 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8041 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     8101 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     8161 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8221 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8281 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt
     8341 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     8401 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg
     8461 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8521 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8581 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8641 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8701 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8761 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8821 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8881 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8941 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9001 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9061 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9121 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9181 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9241 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9301 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     9361 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9421 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9481 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9541 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9601 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9661 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9721 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9781 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9841 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9901 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9961 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10021 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10081 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10141 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10201 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10261 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10321 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10381 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10441 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10501 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10561 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10621 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
    10681 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
    10741 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
    10801 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
    10861 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
    10921 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    10981 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    11041 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
    11101 agctg
//
