LOCUS       V035060                10714 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035060
VERSION     V035060
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4740
                     /label="FGFR3(NM_001163213)"
                     /note="FGFR3(NM_001163213)"
                     /gene="FGFR3"
     CDS             4747..4812
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4856..5067
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5080..5348
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5377..5386
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5407..6069
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6139..6192
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6193..6792
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6793..7381
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7455..7688
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7760..7881
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7921..8056
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8089..8105)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8113..8129
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8137..8167)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8182..8203
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8491..9079)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9250..10110)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10111..10215)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10689..10705
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gcgcccctgc ctgcgccctc
     2341 gcgctctgcg tggccgtggc catcgtggcc ggcgcctcct cggagtcctt ggggacggag
     2401 cagcgcgtcg tggggcgagc ggcagaagtc ccgggcccag agcccggcca gcaggagcag
     2461 ttggtcttcg gcagcgggga tgctgtggag ctgagctgtc ccccgcccgg gggtggtccc
     2521 atggggccca ctgtctgggt caaggatggc acagggctgg tgccctcgga gcgtgtcctg
     2581 gtggggcccc agcggctgca ggtgctgaat gcctcccacg aggactccgg ggcctacagc
     2641 tgccggcagc ggctcacgca gcgcgtactg tgccacttca gtgtgcgggt gacagacgct
     2701 ccatcctcgg gagatgacga agacggggag gacgaggctg aggacacagg tgtggacaca
     2761 ggggcccctt actggacacg gcccgagcgg atggacaaga agctgctggc cgtgccggcc
     2821 gccaacaccg tccgcttccg ctgcccagcc gctggcaacc ccactccctc catctcctgg
     2881 ctgaagaacg gcagggagtt ccgcggcgag caccgcattg gaggcatcaa gctgcggcat
     2941 cagcagtgga gcctggtcat ggaaagcgtg gtgccctcgg accgcggcaa ctacacctgc
     3001 gtcgtggaga acaagtttgg cagcatccgg cagacgtaca cgctggacgt gctggagcgc
     3061 tccccgcacc ggcccatcct gcaggcgggg ctgccggcca accagacggc ggtgctgggc
     3121 agcgacgtgg agttccactg caaggtgtac agtgacgcac agccccacat ccagtggctc
     3181 aagcacgtgg aggtgaatgg cagcaaggtg ggcccggacg gcacacccta cgttaccgtg
     3241 ctcaagtcct ggatcagtga gagtgtggag gccgacgtgc gcctccgcct ggccaatgtg
     3301 tcggagcggg acgggggcga gtacctctgt cgagccacca atttcatagg cgtggccgag
     3361 aaggcctttt ggctgagcgt tcacgggccc cgagcagccg aggaggagct ggtggaggct
     3421 gacgaggcgg gcagtgtgta tgcaggcatc ctcagctacg gggtgggctt cttcctgttc
     3481 atcctggtgg tggcggctgt gacgctctgc cgcctgcgca gcccccccaa gaaaggcctg
     3541 ggctccccca ccgtgcacaa gatctcccgc ttcccgctca agcgacaggt gtccctggag
     3601 tccaacgcgt ccatgagctc caacacacca ctggtgcgca tcgcaaggct gtcctcaggg
     3661 gagggcccca cgctggccaa tgtctccgag ctcgagctgc ctgccgaccc caaatgggag
     3721 ctgtctcggg cccggctgac cctgggcaag ccccttgggg agggctgctt cggccaggtg
     3781 gtcatggcgg aggccatcgg cattgacaag gaccgggccg ccaagcctgt caccgtagcc
     3841 gtgaagatgc tgaaagacga tgccactgac aaggacctgt cggacctggt gtctgagatg
     3901 gagatgatga agatgatcgg gaaacacaaa aacatcatca acctgctggg cgcctgcacg
     3961 cagggcgggc ccctgtacgt gctggtggag tacgcggcca agggtaacct gcgggagttt
     4021 ctgcgggcgc ggcggccccc gggcctggac tactccttcg acacctgcaa gccgcccgag
     4081 gagcagctca ccttcaagga cctggtgtcc tgtgcctacc aggtggcccg gggcatggag
     4141 tacttggcct cccagaagtg catccacagg gacctggctg cccgcaatgt gctggtgacc
     4201 gaggacaacg tgatgaagat cgcagacttc gggctggccc gggacgtgca caacctcgac
     4261 tactacaaga agacgaccaa cggccggctg cccgtgaagt ggatggcgcc tgaggccttg
     4321 tttgaccgag tctacactca ccagagtgac gtctggtcct ttggggtcct gctctgggag
     4381 atcttcacgc tggggggctc cccgtacccc ggcatccctg tggaggagct cttcaagctg
     4441 ctgaaggagg gccaccgcat ggacaagccc gccaactgca cacacgacct gtacatgatc
     4501 atgcgggagt gctggcatgc cgcgccctcc cagaggccca ccttcaagca gctggtggag
     4561 gacctggacc gtgtccttac cgtgacgtcc accgacgagt acctggacct gtcggcgcct
     4621 ttcgagcagt actccccggg tggccaggac acccccagct ccagctcctc aggggacgac
     4681 tccgtgtttg cccacgacct gctgcccccg gccccaccca gcagtggggg ctcgcggacg
     4741 ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     4801 gacgatgaca agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     4861 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     4921 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     4981 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     5041 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     5101 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     5161 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     5221 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     5281 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     5341 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc
     5401 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
     5461 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag
     5521 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
     5581 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
     5641 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
     5701 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc
     5761 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
     5821 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc
     5881 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc
     5941 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
     6001 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc
     6061 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc
     6121 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag
     6181 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     6241 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     6301 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     6361 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6421 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6481 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6541 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6601 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6661 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6721 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6781 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6841 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6901 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6961 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7021 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7081 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7141 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     7201 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7261 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7321 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7381 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7441 agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc tgctttttgc
     7501 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7561 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7621 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7681 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7741 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     7801 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7861 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     7921 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7981 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8041 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt
     8101 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8161 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8221 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8281 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8341 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8401 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8461 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8521 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8581 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8641 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8701 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8761 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8821 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8881 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     8941 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9001 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9061 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9121 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9181 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9241 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9301 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9361 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9421 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9481 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9541 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9601 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9661 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9721 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9781 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9841 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9901 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9961 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10021 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10081 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10141 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10201 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    10261 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    10321 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    10381 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    10441 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    10501 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
    10561 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    10621 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    10681 acgacgttgt aaaacgacgg ccagtgccaa gctg
//
