LOCUS       V045885                10311 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045885
VERSION     V045885
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..4498
                     /label="FAM111B(NM_198947)"
                     /note="FAM111B(NM_198947)"
                     /gene="FAM111B"
     CDS             4520..5239
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        5281..5492
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5505..5773
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5800..6399
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6406..6994
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7068..7301
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7373..7494
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7513..7648
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7686..7702)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7710..7726
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7734..7764)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7779..7800
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8088..8676)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8847..9707)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9708..9812)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10286..10302
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatga attccatgaa gactgaagaa aacaagtcat ttagcgctat
     2341 ggaagatgac cagaggacta gacctgaagt ttcaaaggat actgtcatga agcagacaca
     2401 tgctgacaca cctgttgatc attgtctatc tggcataaga aagtgtagca gcacctttaa
     2461 gcttaaaagt gaagtcaaca agcatgaaac agcccttgaa atgcagaatc caaatttgaa
     2521 caataaagaa tgttgtttca cctttacgtt gaatggaaac tccagaaaat tagaccgtag
     2581 tgtgtttaca gcatatggta aacccagcga gagtatctac tcagccctga gtgctaatga
     2641 ctatttcagt gaaaggataa agaatcagtt taataagaac attattgttt atgaagaaaa
     2701 gacaatagat ggacatataa atttaggaat gcctctcaag tgcctgccta gtgattctca
     2761 ttttaaaatt acatttggtc aaagaaagag tagcaaagaa gatggacaca tattacgcca
     2821 atgtgaaaat ccaaacatgg aatgcattct ttttcatgtt gttgctatag gaaggacaag
     2881 aaagaagatt gttaagatca acgaacttca tgaaaaagga agtaaacttt gtatttatgc
     2941 cttgaagggt gagactattg aaggagcctt atgcaaggat ggccgttttc ggtctgacat
     3001 aggtgaattt gaatggaaac taaaggaagg tcataagaaa atttatggaa aacagtccat
     3061 ggtggatgaa gtatctggaa aagtcttaga aatggacatt tcaaaaaaaa aagcattaca
     3121 acagaaagat atccataaaa aaattaaaca gaatgaaagt gccactgatg aaattaatca
     3181 ccagagtctg atacagtcta agaaaaaagt ccacaaacca aagaaagatg gagagaccaa
     3241 agatgtagaa cacagcagag agcaaattct cccacctcag gatctaagcc attatattaa
     3301 agataaaact cgccagacaa ttcccaggat tagaaattat tacttttgta gtttgccccg
     3361 aaaatatagg caaataaact cacaagttag acggaggccg catctgggta ggcggtatgc
     3421 tattaatctg gatgtccaaa aggaggcaat taatctctta aagaattatc aaacgttgaa
     3481 tgaagccata atgcatcagt atccgaattt taaagaggag gcacagtggg taagaaaata
     3541 ttttcgggaa gaacaaaaga gaatgaatct ttcaccagct aagcaattca acatatataa
     3601 aaaggacttc ggaaaaatga ctgcaaattc tgtttcagtt gcaacctgcg aacagcttac
     3661 atattatagc aagtcagttg ggttcatgca atgggacaat aatggaaaca caggtaatgc
     3721 tacttgcttt gtcttcaatg gtggttatat tttcacctgt cgacatgttg tacatcttat
     3781 ggtgggtaaa aacacacatc caagtttgtg gccagatata attagcaaat gtgcgaaggt
     3841 aaccttcact tatacagagt tctgccctac tcctgacaat tggttttcca ttgagccatg
     3901 gcttaaagtg tccaatgaaa atctagatta tgccatttta aaactaaaag aaaatggaaa
     3961 tgcgtttcct ccaggactat ggcgacagat ttctcctcaa ccatctactg gtttgattta
     4021 tttaattggt catcctgaag gccagatcaa gaaaatagat ggttgtactg tgattcctct
     4081 aaacgaacga ttgaaaaaat atccaaacga ttgtcaagat gggttggtag atctctatga
     4141 taccaccagt aatgtatact gtatgtttac ccaaagaagt ttcctatcag aggtttggaa
     4201 cacacacacg cttagttatg atacttgttt ctctgatggg tcctcaggct ccccagtgtt
     4261 taatgcatct ggcaaattgg ttgctttgca tacctttggg cttttttatc aacgaggatt
     4321 taatgtgcat gcccttattg aatttggtta ttctatggat tctattcttt gtgatattaa
     4381 aaagacaaat gagagcttgt ataaatcatt aaatgatgag aaacttgaga cctacgatga
     4441 agagaaaggt aaacaagagt catcacttca agatcatcag attgaaccca tggaatgtgg
     4501 atccggagga gggggaagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4561 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4621 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     4681 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     4741 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4801 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4861 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4921 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     4981 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     5041 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     5101 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     5161 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     5221 ggacgagctg tacaagtaag cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt
     5281 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     5341 cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     5401 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     5461 tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc
     5521 cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc
     5581 cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca
     5641 ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct
     5701 ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc
     5761 gccgttacag atccaagctg tgaccggcgc ctacgctaga tgaccgagta caagcccacg
     5821 gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc
     5881 gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc
     5941 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     6001 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     6061 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     6121 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     6181 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     6241 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     6301 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     6361 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag tcgacaatca acctctggat
     6421 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6481 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6541 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6601 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     6661 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     6721 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     6781 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     6841 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6901 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6961 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
     7021 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     7081 tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca
     7141 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7201 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7261 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     7321 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     7381 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7441 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7501 ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7561 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7621 aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa
     7681 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7741 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7801 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7861 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7921 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7981 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8041 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8101 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8161 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8221 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8281 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8341 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8401 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8461 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8521 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8581 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8641 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8701 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8761 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8821 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8881 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8941 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9001 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9061 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9121 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9181 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9241 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9301 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9361 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9421 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9481 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9541 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9601 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9661 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9721 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9781 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9841 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     9901 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     9961 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
    10021 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
    10081 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
    10141 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
    10201 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
    10261 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
//
