LOCUS       V037410                11203 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037410
VERSION     V037410
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5230
                     /label="EPHA2(NM_004431)"
                     /note="EPHA2(NM_004431)"
                     /gene="EPHA2"
     CDS             5231..5257
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5261..5287
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5291..5317
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5362..5573
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5586..5854
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5883..5892
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5913..6575
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6645..6698
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6699..7298
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7299..7886
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7960..8193
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8265..8386
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8405..8540
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8578..8594)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8602..8618
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8626..8656)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8671..8692
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8980..9568)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9739..10599)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10600..10704)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11178..11194
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggagct ccaggcagcc cgcgcctgct tcgccctgct
     2341 gtggggctgt gcgctggccg cggccgcggc ggcgcagggc aaggaagtgg tactgctgga
     2401 ctttgctgca gctggagggg agctcggctg gctcacacac ccgtatggca aagggtggga
     2461 cctgatgcag aacatcatga atgacatgcc gatctacatg tactccgtgt gcaacgtgat
     2521 gtctggcgac caggacaact ggctccgcac caactgggtg taccgaggag aggctgagcg
     2581 tatcttcatt gagctcaagt ttactgtacg tgactgcaac agcttccctg gtggcgccag
     2641 ctcctgcaag gagactttca acctctacta tgccgagtcg gacctggact acggcaccaa
     2701 cttccagaag cgcctgttca ccaagattga caccattgcg cccgatgaga tcaccgtcag
     2761 cagcgacttc gaggcacgcc acgtgaagct gaacgtggag gagcgctccg tggggccgct
     2821 cacccgcaaa ggcttctacc tggccttcca ggatatcggt gcctgtgtgg cgctgctctc
     2881 cgtccgtgtc tactacaaga agtgccccga gctgctgcag ggcctggccc acttccctga
     2941 gaccatcgcc ggctctgatg caccttccct ggccactgtg gccggcacct gtgtggacca
     3001 tgccgtggtg ccaccggggg gtgaagagcc ccgtatgcac tgtgcagtgg atggcgagtg
     3061 gctggtgccc attgggcagt gcctgtgcca ggcaggctac gagaaggtgg aggatgcctg
     3121 ccaggcctgc tcgcctggat tttttaagtt tgaggcatct gagagcccct gcttggagtg
     3181 ccctgagcac acgctgccat cccctgaggg tgccacctcc tgcgagtgtg aggaaggctt
     3241 cttccgggca cctcaggacc cagcgtcgat gccttgcaca cgacccccct ccgccccaca
     3301 ctacctcaca gccgtgggca tgggtgccaa ggtggagctg cgctggacgc cccctcagga
     3361 cagcgggggc cgcgaggaca ttgtctacag cgtcacctgc gaacagtgct ggcccgagtc
     3421 tggggaatgc gggccgtgtg aggccagtgt gcgctactcg gagcctcctc acggactgac
     3481 ccgcaccagt gtgacagtga gcgacctgga gccccacatg aactacacct tcaccgtgga
     3541 ggcccgcaat ggcgtctcag gcctggtaac cagccgcagc ttccgtactg ccagtgtcag
     3601 catcaaccag acagagcccc ccaaggtgag gctggagggc cgcagcacca cctcgcttag
     3661 cgtctcctgg agcatccccc cgccgcagca gagccgagtg tggaagtacg aggtcactta
     3721 ccgcaagaag ggagactcca acagctacaa tgtgcgccgc accgagggtt tctccgtgac
     3781 cctggacgac ctggccccag acaccaccta cctggtccag gtgcaggcac tgacgcagga
     3841 gggccagggg gccggcagca aggtgcacga attccagacg ctgtccccgg agggatctgg
     3901 caacttggcg gtgattggcg gcgtggctgt cggtgtggtc ctgcttctgg tgctggcagg
     3961 agttggcttc tttatccacc gcaggaggaa gaaccagcgt gcccgccagt ccccggagga
     4021 cgtttacttc tccaagtcag aacaactgaa gcccctgaag acatacgtgg acccccacac
     4081 atatgaggac cccaaccagg ctgtgttgaa gttcactacc gagatccatc catcctgtgt
     4141 cactcggcag aaggtgatcg gagcaggaga gtttggggag gtgtacaagg gcatgctgaa
     4201 gacatcctcg gggaagaagg aggtgccggt ggccatcaag acgctgaaag ccggctacac
     4261 agagaagcag cgagtggact tcctcggcga ggccggcatc atgggccagt tcagccacca
     4321 caacatcatc cgcctagagg gcgtcatctc caaatacaag cccatgatga tcatcactga
     4381 gtacatggag aatggggccc tggacaagtt ccttcgggag aaggatggcg agttcagcgt
     4441 gctgcagctg gtgggcatgc tgcggggcat cgcagctggc atgaagtacc tggccaacat
     4501 gaactatgtg caccgtgacc tggctgcccg caacatcctc gtcaacagca acctggtctg
     4561 caaggtgtct gactttggcc tgtcccgcgt gctggaggac gaccccgagg ccacctacac
     4621 caccagtggc ggcaagatcc ccatccgctg gaccgccccg gaggccattt cctaccggaa
     4681 gttcacctct gccagcgacg tgtggagctt tggcattgtc atgtgggagg tgatgaccta
     4741 tggcgagcgg ccctactggg agttgtccaa ccacgaggtg atgaaagcca tcaatgatgg
     4801 cttccggctc cccacaccca tggactgccc ctccgccatc taccagctca tgatgcagtg
     4861 ctggcagcag gagcgtgccc gccgccccaa gttcgctgac atcgtcagca tcctggacaa
     4921 gctcattcgt gcccctgact ccctcaagac cctggctgac tttgaccccc gcgtgtctat
     4981 ccggctcccc agcacgagcg gctcggaggg ggtgcccttc cgcacggtgt ccgagtggct
     5041 ggagtccatc aagatgcagc agtatacgga gcacttcatg gcggccggct acactgccat
     5101 cgagaaggtg gtgcagatga ccaacgacga catcaagagg attggggtgc ggctgcccgg
     5161 ccaccagaag cgcatcgcct acagcctgct gggactcaag gaccaggtga acactgtggg
     5221 gatccccatc tacccatacg acgtacctga ttatgcaact tatccttatg atgtacctga
     5281 ttatgctagc tatccttatg atgtcccaga ctacgcatag gcggccgcga aggatctgcg
     5341 atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     5401 ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa
     5461 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     5521 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc
     5581 ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac
     5641 gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg
     5701 tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc
     5761 tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct
     5821 ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag
     5881 acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag tgccgcatca
     5941 ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc acccccaagc
     6001 agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc agcccctacc
     6061 tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc agcggctacg
     6121 agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc atcgagaagt
     6181 acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga
     6241 tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc ttcaccgaca
     6301 agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat aacgtgctgg
     6361 tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg
     6421 acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac gggggcccca
     6481 tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc atcgtggagt
     6541 accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt
     6601 ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc agaggaagtc
     6661 ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac aagcccacgg
     6721 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     6781 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     6841 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     6901 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     6961 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     7021 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     7081 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     7141 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     7201 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     7261 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa
     7321 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     7381 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     7441 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     7501 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     7561 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     7621 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     7681 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     7741 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     7801 gcggcctgct gccggctctg cggcctcttc cgcgtctccg ccttcgccct cagacgagtc
     7861 ggatctccct ttggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag
     7921 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7981 aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     8041 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     8101 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8161 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     8221 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     8281 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8341 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     8401 agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8461 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8521 aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt aatcatggtc
     8581 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8641 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8701 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8761 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8821 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8881 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8941 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     9001 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9061 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9121 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9181 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9241 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9301 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9361 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9421 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9481 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9541 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9601 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9661 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9721 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9781 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9841 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9901 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9961 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    10021 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    10081 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    10141 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    10201 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10261 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10321 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10381 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10441 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10501 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10561 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10621 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10681 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    10741 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    10801 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    10861 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    10921 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    10981 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
    11041 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    11101 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    11161 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
