LOCUS V030837 9436 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030837 VERSION V030837 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..4290 /label="ELF4(NM_001127197)" /note="ELF4(NM_001127197)" /gene="ELF4" CDS 4297..4362 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4406..4617 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4630..4898 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4925..5524 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5531..6119 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6193..6426 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6498..6619 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6638..6773 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6811..6827) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6835..6851 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6859..6889) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6904..6925 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7213..7801) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7972..8832) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8833..8937) /gene="bla" /label="AmpR promoter" primer_bind 9411..9427 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggctatt accctacagc ccagtgacct gatctttgag 2341 ttcgcaagca acgggatgga tgatgatatc caccagctgg aagacccctc tgtgttccca 2401 gctgtgatcg tggagcaggt accctaccct gatttactgc atctgtactc gggactggag 2461 ttggacgacg ttcacaatgg catcataaca gacgggacct tgtgcatgac gcaggatcag 2521 atcctggaag gcagtttttt gctgacagat gacaatgagg ccacctcgca caccatgtca 2581 accgcggaag tcttactcaa tatggagtct cccagcgata tcctggatga gaagcagatc 2641 ttcagtacct ccgaaatgct tccagactcg gaccctgcac cagctgtcac tctgcccaac 2701 tacctgtttc ctgcctctga gcccgatgcc ctgaacaggg cgggtgacac tagtgaccag 2761 gaggggcatt ctctggagga gaaggcctcc agagaggaaa gtgccaagaa gactgggaaa 2821 tcaaagaaga gaatccggaa gaccaagggc aaccgaagta cctcacctgt cactgacccc 2881 agcatcccca ttaggaagaa atcaaaggat ggcaaaggca gcaccatcta tctgtgggag 2941 ttcctcctgg ctcttctgca agacagaaac acctgtccca agtacatcaa gtggacccag 3001 cgagagaaag gcatcttcaa actggtggac tccaaagctg tgtccaagct gtgggggaag 3061 cagaaaaaca agcctgacat gaactatgag acaatggggc gggcactaag atactactac 3121 caaagaggca tactggccaa agtggaaggg cagaggctgg tgtaccagtt taaggagatg 3181 cccaaggacc tggtggtcat tgaagatgag gatgagagca gcgaagccac agcagcccca 3241 cctcaggcct ccacggcctc tgtggcctct gccagtacca cccggcgaac cagctccagg 3301 gtctcatcca gatctgcccc ccagggcaag ggcagctctt cttgggagaa gccaaaaatt 3361 cagcatgtcg gtctccagcc atctgcgagt ctggaattgg gaccgtcgct agacgaggag 3421 atccccacta cctccaccat gctcgtctct ccagcagagg gccaggtcaa gctcaccaaa 3481 gctgtgagtg catcttcagt gcccagcaac atccacctag gagtggcccc cgtggggtcg 3541 ggctcggccc tgaccctgca gacgatccca ctgaccacgg tgctgaccaa tgggcctcct 3601 gccagtacta ctgctcccac tcagctcgtt ctccagagtg ttccagcggc ctctactttc 3661 aaggacacct tcactttgca ggcctctttc cccctgaacg ccagtttcca agacagccag 3721 gtggcagccc caggggctcc actgattctc agtggcctcc cccaacttct ggctggggcc 3781 aaccgtccga ccaacccggc gccacccacg gtcacagggg ctggaccagc agggcccagc 3841 tctcagcccc ctgggactgt cattgctgcc ttcatcagga cttctggcac tacagcagcc 3901 cctagggtca aggaggggcc actgaggtcc tcctcctatg ttcagggtat ggtgacgggg 3961 gcccccatgg aggggctgct ggttcctgaa gagaccctga gggagctcct gagagatcag 4021 gctcatcttc agccacttcc aacccaggtg gtttccaggg gttcccacaa tccgagcctt 4081 ctgggcaacc agactttgtc tcctcccagc cgccccactg ttgggctgac cccagtggct 4141 gaacttgagc tctcctcagg ctcagggtcc ctgctgatgg ctgagcctag tgtgaccaca 4201 tctgggagcc ttctgacaag atcccccacc ccagcccctt tctccccatt caaccctact 4261 tccctcatta agatggagcc ccatgacata ggatccgact acaaagacca tgacggtgat 4321 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc gcgaaggatc 4381 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag 4441 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg 4501 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata 4561 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg 4621 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat 4681 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc 4741 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg 4801 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac 4861 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg 4921 ctagatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc 4981 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc 5041 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct 5101 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc 5161 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag 5221 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa 5281 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct 5341 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt 5401 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac 5461 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc 5521 ctgagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa 5581 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat 5641 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta 5701 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc 5761 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt 5821 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg 5881 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc 5941 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc 6001 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct 6061 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc 6121 tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag 6181 aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt 6241 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga 6301 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc 6361 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct 6421 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa 6481 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata 6541 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 6601 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag 6661 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 6721 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt 6781 ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 6841 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 6901 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 6961 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 7021 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 7081 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 7141 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 7201 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 7261 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 7321 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 7381 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 7441 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 7501 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 7561 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 7621 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 7681 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 7741 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 7801 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 7861 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 7921 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 7981 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 8041 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 8101 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 8161 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 8221 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 8281 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 8341 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 8401 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 8461 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 8521 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 8581 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 8641 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 8701 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 8761 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 8821 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 8881 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 8941 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 9001 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 9061 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 9121 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 9181 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 9241 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg 9301 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 9361 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 9421 ggccagtgcc aagctg //