LOCUS       V033866                11469 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033866
VERSION     V033866
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5448
                     /label="ITGA5(NM_002205)"
                     /note="ITGA5(NM_002205)"
                     /gene="ITGA5"
     CDS             5488..6198
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     promoter        6238..6449
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6462..6730
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6763..7557
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    7564..8152
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8226..8459
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8531..8652
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8671..8806
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8844..8860)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8868..8884
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8892..8922)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8937..8958
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9246..9834)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10005..10865)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10866..10970)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11444..11460
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggggagc cggacgccag agtcccctct ccacgccgtg
     2341 cagctgcgct ggggcccccg gcgccgaccc ccgctgctgc cgctgctgtt gctgctgctg
     2401 ccgccgccac ccagggtcgg gggcttcaac ttagacgcgg aggccccagc agtactctcg
     2461 gggcccccgg gctccttctt cggattctca gtggagtttt accggccggg aacagacggg
     2521 gtcagtgtgc tggtgggagc acccaaggct aataccagcc agccaggagt gctgcagggt
     2581 ggtgctgtct acctctgtcc ttggggtgcc agccccacac agtgcacccc cattgaattt
     2641 gacagcaaag gctctcggct cctggagtcc tcactgtcca gctcagaggg agaggagcct
     2701 gtggagtaca agtccttgca gtggttcggg gcaacagttc gagcccatgg ctcctccatc
     2761 ttggcatgcg ctccactgta cagctggcgc acagagaagg agccactgag cgaccccgtg
     2821 ggcacctgct acctctccac agataacttc acccgaattc tggagtatgc accctgccgc
     2881 tcagatttca gctgggcagc aggacagggt tactgccaag gaggcttcag tgccgagttc
     2941 accaagactg gccgtgtggt tttaggtgga ccaggaagct atttctggca aggccagatc
     3001 ctgtctgcca ctcaggagca gattgcagaa tcttattacc ccgagtacct gatcaacctg
     3061 gttcaggggc agctgcagac tcgccaggcc agttccatct atgatgacag ctacctagga
     3121 tactctgtgg ctgttggtga attcagtggt gatgacacag aagactttgt tgctggtgtg
     3181 cccaaaggga acctcactta cggctatgtc accatcctta atggctcaga cattcgatcc
     3241 ctctacaact tctcagggga acagatggcc tcctactttg gctatgcagt ggccgccaca
     3301 gacgtcaatg gggacgggct ggatgacttg ctggtggggg cacccctgct catggatcgg
     3361 acccctgacg ggcggcctca ggaggtgggc agggtctacg tctacctgca gcacccagcc
     3421 ggcatagagc ccacgcccac ccttaccctc actggccatg atgagtttgg ccgatttggc
     3481 agctccttga cccccctggg ggacctggac caggatggct acaatgatgt ggccatcggg
     3541 gctccctttg gtggggagac ccagcaggga gtagtgtttg tatttcctgg gggcccagga
     3601 gggctgggct ctaagccttc ccaggttctg cagcccctgt gggcagccag ccacacccca
     3661 gacttctttg gctctgccct tcgaggaggc cgagacctgg atggcaatgg atatcctgat
     3721 ctgattgtgg ggtcctttgg tgtggacaag gctgtggtat acaggggccg ccccatcgtg
     3781 tccgctagtg cctccctcac catcttcccc gccatgttca acccagagga gcggagctgc
     3841 agcttagagg ggaaccctgt ggcctgcatc aaccttagct tctgcctcaa tgcttctgga
     3901 aaacacgttg ctgactccat tggtttcaca gtggaacttc agctggactg gcagaagcag
     3961 aagggagggg tacggcgggc actgttcctg gcctccaggc aggcaaccct gacccagacc
     4021 ctgctcatcc agaatggggc tcgagaggat tgcagagaga tgaagatcta cctcaggaac
     4081 gagtcagaat ttcgagacaa actctcgccg attcacatcg ctctcaactt ctccttggac
     4141 ccccaagccc cagtggacag ccacggcctc aggccagccc tacattatca gagcaagagc
     4201 cggatagagg acaaggctca gatcttgctg gactgtggag aagacaacat ctgtgtgcct
     4261 gacctgcagc tggaagtgtt tggggagcag aaccatgtgt acctgggtga caagaatgcc
     4321 ctgaacctca ctttccatgc ccagaatgtg ggtgagggtg gcgcctatga ggctgagctt
     4381 cgggtcaccg cccctccaga ggctgagtac tcaggactcg tcagacaccc agggaacttc
     4441 tccagcctga gctgtgacta ctttgccgtg aaccagagcc gcctgctggt gtgtgacctg
     4501 ggcaacccca tgaaggcagg agccagtctg tggggtggcc ttcggtttac agtccctcat
     4561 ctccgggaca ctaagaaaac catccagttt gacttccaga tcctcagcaa gaatctcaac
     4621 aactcgcaaa gcgacgtggt ttcctttcgg ctctccgtgg aggctcaggc ccaggtcacc
     4681 ctgaacggtg tctccaagcc tgaggcagtg ctattcccag taagcgactg gcatccccga
     4741 gaccagcctc agaaggagga ggacctggga cctgctgtcc accatgtcta tgagctcatc
     4801 aaccaaggcc ccagctccat tagccagggt gtgctggaac tcagctgtcc ccaggctctg
     4861 gaaggtcagc agctcctata tgtgaccaga gttacgggac tcaactgcac caccaatcac
     4921 cccattaacc caaagggcct ggagttggat cccgagggtt ccctgcacca ccagcaaaaa
     4981 cgggaagctc caagccgcag ctctgcttcc tcgggacctc agatcctgaa atgcccggag
     5041 gctgagtgtt tcaggctgcg ctgtgagctc gggcccctgc accaacaaga gagccaaagt
     5101 ctgcagttgc atttccgagt ctgggccaag actttcttgc agcgggagca ccagccattt
     5161 agcctgcagt gtgaggctgt gtacaaagcc ctgaagatgc cctaccgaat cctgcctcgg
     5221 cagctgcccc aaaaagagcg tcaggtggcc acagctgtgc aatggaccaa ggcagaaggc
     5281 agctatggcg tcccactgtg gatcatcatc ctagccatcc tgtttggcct cctgctccta
     5341 ggtctactca tctacatcct ctacaagctt ggattcttca aacgctccct cccatatggc
     5401 accgccatgg aaaaagctca gctcaagcct ccagccacct ctgatgccgc ggccgcaggt
     5461 ggaggcggat caggaggcgg tggatctatg gtgagcaagg gcgaggagga taacatggcc
     5521 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     5581 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     5641 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     5701 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     5761 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     5821 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     5881 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     5941 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     6001 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     6061 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     6121 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     6181 gacgagctgt acaagtaggg ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg
     6241 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg
     6301 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     6361 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     6421 ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt
     6481 cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc
     6541 ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt
     6601 cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca
     6661 cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc
     6721 gttacagatc caagctgtga ccggcgccta cgctaggcca ccatggctga acaagatgga
     6781 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     6841 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     6901 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     6961 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     7021 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     7081 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     7141 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     7201 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     7261 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     7321 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     7381 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     7441 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     7501 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagtc
     7561 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7621 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7681 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7741 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7801 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7861 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7921 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     7981 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     8041 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     8101 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc
     8161 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8221 gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg
     8281 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8341 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8401 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8461 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     8521 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     8581 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8641 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc
     8701 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8761 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga
     8821 gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     8881 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     8941 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     9001 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     9061 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9121 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     9181 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9241 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9301 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9361 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9421 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9481 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9541 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9601 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9661 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     9721 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9781 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9841 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9901 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9961 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10021 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10081 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10141 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10201 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10261 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10321 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10381 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10441 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10501 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10561 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10621 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10681 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10741 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10801 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10861 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10921 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10981 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
    11041 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
    11101 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
    11161 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
    11221 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
    11281 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
    11341 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
    11401 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
    11461 gccaagctg
//
