LOCUS V042473 10138 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V042473 VERSION V042473 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..4839 /label="EXO1(NM_006027)" /note="EXO1(NM_006027)" /gene="EXO1" CDS 4840..4863 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" promoter 4907..5118 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 5131..5399 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 5432..6226 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" misc_feature 6233..6821 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6895..7128 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7200..7321 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7340..7475 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7513..7529) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7537..7553 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7561..7591) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7606..7627 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7915..8503) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8674..9534) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9535..9639) /gene="bla" /label="AmpR promoter" primer_bind 10113..10129 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggggata cagggattgc tacaatttat caaagaagct 2341 tcagaaccca tccatgtgag gaagtataaa gggcaggtag tagctgtgga tacatattgc 2401 tggcttcaca aaggagctat tgcttgtgct gaaaaactag ccaaaggtga acctactgat 2461 aggtatgtag gattttgtat gaaatttgta aatatgttac tatctcatgg gatcaagcct 2521 attctcgtat ttgatggatg tactttacct tctaaaaagg aagtagagag atctagaaga 2581 gaaagacgac aagccaatct tcttaaggga aagcaacttc ttcgtgaggg gaaagtctcg 2641 gaagctcgag agtgtttcac ccggtctatc aatatcacac atgccatggc ccacaaagta 2701 attaaagctg cccggtctca gggggtagat tgcctcgtgg ctccctatga agctgatgcg 2761 cagttggcct atcttaacaa agcgggaatt gtgcaagcca taattacaga ggactcggat 2821 ctcctagctt ttggctgtaa aaaggtaatt ttaaagatgg accagtttgg aaatggactt 2881 gaaattgatc aagctcggct aggaatgtgc agacagcttg gggatgtatt cacggaagag 2941 aagtttcgtt acatgtgtat tctttcaggt tgtgactacc tgtcatcact gcgtgggatt 3001 ggattagcaa aggcatgcaa agtcctaaga ctagccaata atccagatat agtaaaggtt 3061 atcaagaaaa ttggacatta tctcaagatg aatatcacgg taccagagga ttacatcaac 3121 gggtttattc gggccaacaa taccttcctc tatcagctag tttttgatcc catcaaaagg 3181 aaacttattc ctctgaacgc ctatgaagat gatgttgatc ctgaaacact aagctacgct 3241 gggcaatatg ttgatgattc catagctctt caaatagcac ttggaaataa agatataaat 3301 acttttgaac agatcgatga ctacaatcca gacactgcta tgcctgccca ttcaagaagt 3361 catagttggg atgacaaaac atgtcaaaag tcagctaatg ttagcagcat ttggcatagg 3421 aattactctc ccagaccaga gtcgggtact gtttcagatg ccccacaatt gaaggaaaat 3481 ccaagtactg tgggagtgga acgagtgatt agtactaaag ggttaaatct cccaaggaaa 3541 tcatccattg tgaaaagacc aagaagtgca gagctgtcag aagatgacct gttgagtcag 3601 tattctcttt catttacgaa gaagaccaag aaaaatagct ctgaaggcaa taaatcattg 3661 agcttttctg aagtgtttgt gcctgacctg gtaaatggac ctactaacaa aaagagtgta 3721 agcactccac ctaggacgag aaataaattt gcaacatttt tacaaaggaa aaatgaagaa 3781 agtggtgcag ttgtggttcc agggaccaga agcaggtttt tttgcagttc agattctact 3841 gactgtgtat caaacaaagt gagcatccag cctctggatg aaactgctgt cacagataaa 3901 gagaacaatc tgcatgaatc agagtatgga gaccaagaag gcaagagact ggttgacaca 3961 gatgtagcac gtaattcaag tgatgacatt ccgaataatc atattccagg tgatcatatt 4021 ccagacaagg caacagtgtt tacagatgaa gagtcctact cttttgagag cagcaaattt 4081 acaaggacca tttcaccacc cactttggga acactaagaa gttgttttag ttggtctgga 4141 ggtcttggag atttttcaag aacgccgagc ccctctccaa gcacagcatt gcagcagttc 4201 cgaagaaaga gcgattcccc cacctctttg cctgagaata atatgtctga tgtgtcgcag 4261 ttaaagagcg aggagtccag tgacgatgag tctcatccct tacgagaaga ggcatgttct 4321 tcacagtccc aggaaagtgg agaattctca ctgcagagtt caaatgcatc aaagctttct 4381 cagtgctcta gtaaggactc tgattcagag gaatctgatt gcaatattaa gttacttgac 4441 agtcaaagtg accagacctc caagctacgt ttatctcatt tctcaaaaaa agacacacct 4501 ctaaggaaca aggttcctgg gctatataag tccagttctg cagactctct ttctacaacc 4561 aagatcaaac ctctaggacc tgccagagcc agtgggctga gcaagaagcc ggcaagcatc 4621 cagaagagaa agcatcataa tgccgagaac aagccggggt tacagatcaa actcaatgag 4681 ctctggaaaa actttggatt taaaaaagat tctgaaaagc ttcctccttg taagaaaccc 4741 ctgtccccag tcagagataa catccaacta actccagaag cggaagagga tatatttaac 4801 aaacctgaat gtggccgtgt tcaaagagca atattccagg attacaagga cgacgatgac 4861 aagtagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca 4921 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga 4981 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag 5041 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg 5101 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc 5161 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt 5221 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc 5281 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg 5341 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc 5401 aagctgtgac cggcgcctac gctaggccac catggctgaa caagatggat tgcacgcagg 5461 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 5521 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 5581 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct 5641 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 5701 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 5761 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 5821 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 5881 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 5941 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 6001 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 6061 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 6121 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 6181 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagtcg acaatcaacc 6241 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 6301 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 6361 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 6421 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 6481 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 6541 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 6601 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt 6661 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 6721 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 6781 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa 6841 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag 6901 ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt 6961 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 7021 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 7081 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat 7141 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga 7201 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 7261 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 7321 atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc 7381 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 7441 attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa 7501 ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 7561 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 7621 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 7681 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 7741 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 7801 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 7861 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 7921 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 7981 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 8041 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 8101 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 8161 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 8221 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 8281 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 8341 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 8401 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 8461 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 8521 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 8581 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 8641 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 8701 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 8761 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 8821 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 8881 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 8941 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 9001 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 9061 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 9121 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 9181 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 9241 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 9301 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 9361 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 9421 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 9481 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 9541 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 9601 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 9661 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 9721 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 9781 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 9841 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 9901 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 9961 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 10021 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 10081 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg //