LOCUS       V035527                11221 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035527
VERSION     V035527
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2290..5193
                     /label="AARS1(NM_001605)"
                     /note="AARS1(NM_001605)"
                     /gene="AARS1"
     CDS             5230..5949
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        5990..6201
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6214..6482
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6515..7309
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    7316..7904
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7978..8211
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8283..8404
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8423..8558
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8596..8612)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8620..8636
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8644..8674)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8689..8710
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8998..9586)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9757..10617)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10618..10722)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11196..11212
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagccacca tggactctac tctaacagca agtgaaatcc ggcagcgatt tatagatttc
     2341 ttcaagagga acgagcatac gtatgttcac tcgtctgcca ccatcccatt ggatgacccc
     2401 actttgctct ttgccaatgc aggcatgaac cagtttaaac ccattttcct gaacacaatt
     2461 gacccatctc accccatggc aaagctgagc agagctgcca atacccagaa gtgcatccgg
     2521 gctgggggca aacataatga cctggacgat gtgggcaagg atgtctatca tcacaccttc
     2581 ttcgagatgc tgggctcttg gtcttttgga gattacttta aggaattggc atgtaagatg
     2641 gctctggaac tcctcaccca agagtttggc attcccattg aaagacttta tgttacttac
     2701 tttggcgggg atgaagcagc tggcttagaa gcagatctgg aatgcaaaca gatctggcaa
     2761 aatttggggc tggatgacac caaaatcctc ccaggcaaca tgaaggataa cttctgggag
     2821 atgggtgaca cgggcccctg tggtccttgc agtgagatcc actacgaccg gattggtggt
     2881 cgggacgccg cacatcttgt caaccaggac gaccctaatg tgctggagat ctggaacctt
     2941 gtgttcatcc agtataacag ggaagctgat ggcattctga aacctcttcc caagaaaagc
     3001 attgacacag ggatgggcct ggaacgactg gtatctgtgc tgcagaataa gatgtccaac
     3061 tatgacactg acctttttgt cccttacttt gaagccattc agaagggcac aggtgcccga
     3121 ccatacactg ggaaagttgg tgctgaggat gccgatggga ttgacatggc ctaccgggtg
     3181 ctggctgacc acgctcggac catcactgtg gcactggctg atggtggccg gcctgacaac
     3241 acagggcgtg gatatgtgtt gagacggatt ctccgccgag ctgtccgata cgcccatgaa
     3301 aagctcaatg ccagcagggg cttctttgct acgttagtgg atgttgtcgt ccagtccctg
     3361 ggagatgcat ttcctgagct gaagaaggac ccagacatgg tgaaggacat cattaatgaa
     3421 gaagaggtgc agtttctcaa gactctcagc agagggcgtc gcatcctgga caggaaaatt
     3481 cagagcctgg gagacagcaa gaccattccc ggagacactg cttggctcct ctatgacacc
     3541 tatgggtttc cagtggatct gactggactg attgctgaag agaagggcct ggtggtagac
     3601 atggatggct ttgaagagga gaggaaactg gcccagctga aatcacaggg caagggagct
     3661 ggtggggaag acctcattat gctggacatt tacgctatcg aagagctccg ggcacggggt
     3721 ctggaggtca cagatgattc cccaaagtac aattaccatt tggactccag tggtagctat
     3781 gtatttgaga acacagtggc tacggtgatg gctctgcgca gggagaagat gttcgtggaa
     3841 gaggtgtcca caggccagga gtgtggagtg gtgctggaca agacctgttt ctatgctgag
     3901 caaggaggcc agatctatga cgaaggctac ctggtgaagg tggatgacag cagtgaagat
     3961 aaaacagagt ttacagtgaa gaatgctcag gtccgaggag ggtatgtgct acacattgga
     4021 accatctacg gtgacctgaa agtgggggat caggtctggc tgtttattga tgagccccga
     4081 cgaagaccca tcatgagcaa ccacacagct acgcacattc tgaacttcgc cctgcgctca
     4141 gtgcttgggg aagctgacca gaaaggctca ttggttgctc ctgaccgcct cagatttgac
     4201 tttactgcca agggagccat gtccacccaa cagatcaaga aggctgaaga gattgctaat
     4261 gagatgattg aggcagccaa ggccgtctat acccaggatt gccccctggc agcagcgaaa
     4321 gccatccagg gcctacgggc tgtgtttgat gagacctatc ctgaccctgt gcgagtcgtc
     4381 tccattgggg tcccggtgtc cgagttgctg gatgacccct ctgggcctgc tggctccctg
     4441 acttctgttg agttctgtgg gggaacgcac ctgcggaact cgagtcatgc aggagctttt
     4501 gtgatcgtga cggaagaagc cattgccaag ggtatccgga ggattgtggc tgtcacaggt
     4561 gccgaggccc agaaggccct caggaaagca gagagcttga agaaatgtct ctctgtcatg
     4621 gaagccaaag tgaaggctca gactgctcca aacaaggatg tgcagaggga gatcgctgac
     4681 cttggagagg ccctggccac tgcagtcatc ccccagtggc agaaggatga attgcgggag
     4741 actctcaaat ccctaaagaa ggtcatggat gacttggacc gagccagcaa agccgatgtc
     4801 cagaaacgag tgttagagaa gacgaagcag ttcatcgaca gcaaccccaa ccagcctctt
     4861 gtcatcctgg agatggagag cggcgcctca gccaaggccc tgaatgaagc cttgaagctc
     4921 ttcaagatgc actcccctca gacttctgcc atgctcttca cggtggacaa tgaggctggc
     4981 aagatcacgt gcctgtgtca agttccccag aatgcagcca atcggggctt aaaagccagc
     5041 gagtgggtgc agcaggtgtc aggcttgatg gacggtaaag gtggtggcaa ggatgtgtct
     5101 gcacaggcca caggcaagaa cgttggctgc ctgcaggagg cgctgcagct ggccacttcc
     5161 ttcgcccagc tgcgcctcgg ggatgtaaag aacggatccg gtggaggcgg atcaggaggc
     5221 ggtggatcta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     5281 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     5341 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     5401 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     5461 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     5521 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     5581 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     5641 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     5701 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     5761 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     5821 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     5881 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     5941 tacaagtagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     6001 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     6061 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     6121 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     6181 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     6241 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     6301 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     6361 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     6421 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     6481 tccaagctgt gaccggcgcc tacgctaggc caccatggct gaacaagatg gattgcacgc
     6541 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     6601 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     6661 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     6721 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     6781 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     6841 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     6901 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     6961 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     7021 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     7081 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     7141 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     7201 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     7261 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag tcgacaatca
     7321 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7381 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7441 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     7501 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     7561 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     7621 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     7681 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     7741 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     7801 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     7861 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac
     7921 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7981 aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct
     8041 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8101 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8161 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     8221 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     8281 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8341 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8401 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc
     8461 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     8521 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc
     8581 aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8641 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8701 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8761 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8821 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8881 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8941 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     9001 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     9061 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9121 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9181 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9241 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9301 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9361 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9421 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9481 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9541 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9601 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9661 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9721 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9781 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9841 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9901 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9961 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    10021 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    10081 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    10141 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    10201 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10261 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10321 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10381 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10441 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10501 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10561 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10621 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10681 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10741 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    10801 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    10861 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    10921 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    10981 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    11041 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    11101 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    11161 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
    11221 g
//
