LOCUS       V037317                10855 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037317
VERSION     V037317
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4881
                     /label="DNAAF5(NM_017802)"
                     /note="DNAAF5(NM_017802)"
                     /gene="DNAAF5"
     CDS             4888..4953
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4997..5208
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5221..5489
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5518..5527
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5548..6210
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6280..6333
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6334..6933
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6934..7522
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7596..7829
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7901..8022
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8062..8197
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8230..8246)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8254..8270
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8278..8308)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8323..8344
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8632..9220)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9391..10251)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10252..10356)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10830..10846
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cggcgctggg ggtggcggag
     2341 gccgtggcgg ccccacaccc ggctgagggg gccgagacgg ctgaggcggt ggagctgagc
     2401 cgcgccctga gccgcctgct gccggggctg gaggccgaca gcaagccggg ccggcggcgc
     2461 gccttggagg ccctgcggcg cgcgctggag gagccaggcc ctgccgccga ccccaccgct
     2521 ttccagggcc cctgggcgcg cctactgctg ccgcgcttgc tgcgctgcct gagcgacccc
     2581 gccgagggct gccgcgcgct ggcagtgcac ctgctggatc tgggcctgcg ccgcgccgcg
     2641 cggccccgcg atgccctgcc gcgcctgctg cccgcgctcg ccgcgcgctt ggccggcccc
     2701 gtgcccgcgc gccgcccgcc cgaggcctgt gaggagctgc gcctggcgct tgtgcagctg
     2761 ctgggcctgg ccgtggacct gtgcggcgcc gcgctcgcgc cccacctgga cgacgctctg
     2821 cgcgcgctgc gctgctccct gctcgacccc ttcgccgccg tgcgccgcga gagctgcagc
     2881 tgcgccgccg ccctggcgca ggccacgccc gaccacttcc acatgcagtc ggagtctctg
     2941 atcgggcccc tgatgcagac catctcccac cagcactgga aggtccgtgt ggccgccatt
     3001 gaagccacag gcgcagtgat ccattttggc aacgggaagt ccgtggacga cgtgctttcc
     3061 cattttgctc agcgactgtt tgatgacgtc ccgcaggtcc ggcgggcggt ggcctccgtg
     3121 gtgggcggct ggctgctgtg tctgcgtgac cgttactcct tcttccacaa gctcatccct
     3181 ctgctgctca gtagcctcaa cgacgaggtg cctgaggtca ggcagctggc tgccagcctc
     3241 tgggaggacg ttggcctgca gtggcagaag gagaatgagg aggacctgaa ggacaagctg
     3301 gactttgccc ctcccacccc accccattac cctccacatg agcgccgccc tgtgctgggc
     3361 tgccgggagc tcgtcttcag gaacctctcc aagatcctcc ctgccctgtg ccacgacatc
     3421 accgactggg tggtggggac ccgagtgaag tcggcacagc tgctcccagt gctgctgctg
     3481 catgccgagg accacgccac gcagcacctg gaggtcgtcc tccggaccct gttccaggcc
     3541 tgcaccgacg aggaggcagc cgtggtccaa agttgtacca gatccgcaga gctcgtcggg
     3601 acgtttgtca gccctgaggt gtttctgaag ctgatcttat cgacgctgaa gaagacgccc
     3661 tctgcctccg gcctcctggt gctggcctcc gccatgcggg gttgcccccg agaagccctc
     3721 cagccgcacc tggcagccat cgccacagag ctggcacagg cccacatctg ccaggcatct
     3781 gaaaacgacc tctacctgga gcgcctgctg ctgtgtgtgc aggctctggt gtctgtgtgt
     3841 catgaggact gtggcgtggc cagcctgcag ctcttggacg tgctgctgac aatagtggcc
     3901 ctcgcaggtg ctaccggcct gagggacaag gcacaggaga cgatggactc actggccatg
     3961 gtggagggtg tcagcagctg ccaggacctc taccgcaagc acattggtcc cctcctggag
     4021 cgggtgaccg cgtcgcacct tgactggacc gcacactcgc cggagctcct gcagttcagt
     4081 gtcatcgtcg cacagtcagg ccctgccctg ggagaagccc tgccacacgt cgtgcccacg
     4141 ctgagggcct gtctgcagcc ctcccaagac ccgcagatgc gcctgaagct gttctccatc
     4201 ctgtccaccg tgctgctcag agccacggac accatcaact cccaggggca gtttcccagc
     4261 tacctcgaga cggtgacaaa ggacatcctg gcccccaatc tgcagtggca tgcggggagg
     4321 acagccgcgg ccatccgcac ggctgccgtg tcctgcctct gggcgctcac cagcagcgag
     4381 gtcctgtcgg cagagcagat acgggacgtg caggaaacac tgatgcccca ggtcctgacc
     4441 accctggagg aggattcgaa gatgacgcga ctgatctcat gccgtattat caacacgttc
     4501 ttaaaaacct cgggcggcat gacggatcca gagaaactca tcaggattta tcctgaactc
     4561 ttaaaacgcc tagatgacgt gtccaacgat gtgaggatgg cagccgcctc caccttggtc
     4621 acctggctgc agtgtgtcaa gggtgccaac gcaaaatcct actatcagag cagtgtccag
     4681 tacctgtacc gagagttgct ggttcacctt gacgatccag agagggccat ccaggatgca
     4741 attttagagg tcctcaaaga gggcagcggg ctgttcccag atctcctggt gagggagacg
     4801 gaggccgtca tccacaagca ccgctcggcc acctactgcg agcagctcct gcagcatgtg
     4861 caggccgtgc cagccacaca gggatccgac tacaaagacc atgacggtga ttataaagat
     4921 catgacatcg actacaagga tgacgatgac aagtagcggc cgcgaaggat ctgcgatcgc
     4981 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     5041 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     5101 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     5161 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga
     5221 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg
     5281 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag
     5341 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct
     5401 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt
     5461 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc
     5521 accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc
     5581 accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc
     5641 cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg
     5701 agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac
     5761 cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag
     5821 gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc
     5881 gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc
     5941 atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc
     6001 agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc
     6061 cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc
     6121 gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag
     6181 cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc
     6241 gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta
     6301 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc
     6361 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     6421 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     6481 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     6541 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     6601 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     6661 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     6721 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     6781 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     6841 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     6901 acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt
     6961 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     7021 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     7081 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     7141 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     7201 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7261 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7321 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     7381 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7441 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7501 tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt
     7561 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7621 aagataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7681 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7741 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7801 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     7861 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     7921 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7981 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     8041 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     8101 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     8161 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt
     8221 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8281 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8341 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8401 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8461 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8521 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8581 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8641 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8701 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8761 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8821 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8881 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8941 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9001 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9061 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9121 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9181 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9241 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9301 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9361 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9421 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9481 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9541 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9601 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9661 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9721 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9781 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9841 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9901 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9961 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    10021 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10081 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10141 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10201 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10261 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10321 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10381 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    10441 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    10501 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    10561 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
    10621 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
    10681 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    10741 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
    10801 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//
