LOCUS V022976 12602 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V022976 VERSION V022976 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 3970..4023 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 4927..4980 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 4987..7527 /label="CD22(NM_001771)" /note="CD22(NM_001771)" /gene="CD22" promoter 7572..7783 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 7796..8064 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 8091..8690 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 8697..9285 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 9359..9592 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 9664..9785 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 9804..9939 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(9977..9993) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 10001..10017 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(10025..10055) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 10070..10091 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(10379..10967) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(11138..11998) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(11999..12103) /gene="bla" /label="AmpR promoter" primer_bind 12577..12593 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg ccaccatgcc acctcctcgc ctcctcttct tcctcctctt cctcaccccc 2341 atggaagtca ggcccgagga acctctagtg gtgaaggtgg aagagggaga taacgctgtg 2401 ctgcagtgcc tcaaggggac ctcagatggc cccactcagc agctgacctg gtctcgggag 2461 tccccgctta aacccttctt aaaactcagc ctggggctgc caggcctggg aatccacatg 2521 aggcccctgg ccatctggct tttcatcttc aacgtctctc aacagatggg gggcttctac 2581 ctgtgccagc cggggccccc ctctgagaag gcctggcagc ctggctggac agtcaatgtg 2641 gagggcagcg gggagctgtt ccggtggaat gtttcggacc taggtggcct gggctgtggc 2701 ctgaagaaca ggtcctcaga gggccccagc tccccttccg ggaagctcat gagccccaag 2761 ctgtatgtgt gggccaaaga ccgccctgag atctgggagg gagagcctcc gtgtctccca 2821 ccgagggaca gcctgaacca gagcctcagc caggacctca ccatggcccc tggctccaca 2881 ctctggctgt cctgtggggt accccctgac tctgtgtcca ggggccccct ctcctggacc 2941 catgtgcacc ccaaggggcc taagtcattg ctgagcctag agctgaagga cgatcgcccg 3001 gccagagata tgtgggtaat ggagacgggt ctgttgttgc cccgggccac agctcaagac 3061 gctggaaagt attattgtca ccgtggcaac ctgaccatgt cattccacct ggagatcact 3121 gctcggccag tactatggca ctggctgctg aggactggtg gctggaaggt ctcagctgtg 3181 actttggctt atctgatctt ctgcctgtgt tcccttgtgg gcattcttca tcttcaaaga 3241 gccctggtcc tgaggaggaa aagaaagcga atgactgacc ccaccaggag attcttcaaa 3301 gtgacgcctc ccccaggaag cgggccccag aaccagtacg ggaacgtgct gtctctcccc 3361 acacccacct caggcctcgg acgcgcccag cgttgggccg caggcctggg gggcactgcc 3421 ccgtcttatg gaaacccgag cagcgacgtc caggcggatg gagccttggg gtcccggagc 3481 ccgccgggag tgggcccaga agaagaggaa ggggagggct atgaggaacc tgacagtgag 3541 gaggactccg agttctatga gaacgactcc aaccttgggc aggaccagct ctcccaggat 3601 ggcagcggct acgagaaccc tgaggatgag cccctgggtc ctgaggatga agactccttc 3661 tccaacgctg agtcttatga gaacgaggat gaagagctga cccagccggt cgccaggaca 3721 atggacttcc tgagccctca tgggtcagcc tgggacccca gccgggaagc aacctccctg 3781 gggtcccagt cctatgagga tatgagagga atcctgtatg cagcccccca gctccgctcc 3841 attcggggcc agcctggacc caatcatgag gaagatgcag actcttatga gaacatggat 3901 aatcccgatg ggccagaccc agcctgggga ggagggggcc gcatgggcac ctggagcacc 3961 aggagtggag agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc 4021 ccagaattca tgacaacacc cagaaattca gtaaatggga ctttcccggc agagccaatg 4081 aaaggcccta ttgctatgca atctggtcca aaaccactct tcaggaggat gtcttcactg 4141 gtgggcccca cgcaaagctt cttcatgagg gaatctaaga ctttgggggc tgtccagatt 4201 atgaatgggc tcttccacat tgccctgggg ggtcttctga tgatcccagc agggatctat 4261 gcacccatct gtgtgactgt gtggtaccct ctctggggag gcattatgta tattatttcc 4321 ggatcactcc tggcagcaac ggagaaaaac tccaggaagt gtttggtcaa aggaaaaatg 4381 ataatgaatt cattgagcct ctttgctgcc atttctggaa tgattctttc aatcatggac 4441 atacttaata ttaaaatttc ccatttttta aaaatggaga gtctgaattt tattagagct 4501 cacacaccat atattaacat atacaactgt gaaccagcta atccctctga gaaaaactcc 4561 ccatctaccc aatactgtta cagcatacaa tctctgttct tgggcatttt gtcagtgatg 4621 ctgatctttg ccttcttcca ggaacttgta atagctggca tcgttgagaa tgaatggaaa 4681 agaacgtgct ccagacccaa atctaacata gttctcctgt cagcagaaga aaaaaaagaa 4741 cagactattg aaataaaaga agaagtggtt gggctaactg aaacatcttc ccaaccaaag 4801 aatgaagaag acattgaaat tattccaatc caagaagagg aagaagaaga aacagagacg 4861 aactttccag aacctcccca agatcaggaa tcctcaccaa tagaaaatga cagctctcct 4921 agtggagagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa tcctggccca 4981 gaatttatgc atctcctcgg cccctggctc ctgctcctgg ttctagaata cttggctttc 5041 tctgactcaa gtaaatgggt ttttgagcac cctgaaaccc tctacgcctg ggagggggcc 5101 tgcgtctgga tcccctgcac ctacagagcc ctagatggtg acctggaaag cttcatcctg 5161 ttccacaatc ctgagtataa caagaacacc tcgaagtttg atgggacaag actctatgaa 5221 agcacaaagg atgggaaggt tccttctgag cagaaaaggg tgcaattcct gggagacaag 5281 aataagaact gcacactgag tatccacccg gtgcacctca atgacagtgg tcagctgggg 5341 ctgaggatgg agtccaagac tgagaaatgg atggaacgaa tacacctcaa tgtctctgaa 5401 aggccttttc cacctcatat ccagctccct ccagaaattc aagagtccca ggaagtcact 5461 ctgacctgct tgctgaattt ctcctgctat gggtatccga tccaattgca gtggctccta 5521 gagggggttc caatgaggca ggctgctgtc acctcgacct ccttgaccat caagtctgtc 5581 ttcacccgga gcgagctcaa gttctcccca cagtggagtc accatgggaa gattgtgacc 5641 tgccagcttc aggatgcaga tgggaagttc ctctccaatg acacggtgca gctgaacgtg 5701 aagcacaccc cgaagttgga gatcaaggtc actcccagtg atgccatagt gagggagggg 5761 gactctgtga ccatgacctg cgaggtcagc agcagcaacc cggagtacac gacggtatcc 5821 tggctcaagg atgggacctc gctgaagaag cagaatacat tcacgctaaa cctgcgcgaa 5881 gtgaccaagg accagagtgg gaagtactgc tgtcaggtct ccaatgacgt gggcccggga 5941 aggtcggaag aagtgttcct gcaagtgcag tatgccccgg aaccttccac ggttcagatc 6001 ctccactcac cggctgtgga gggaagtcaa gtcgagtttc tttgcatgtc actggccaat 6061 cctcttccaa caaattacac gtggtaccac aatgggaaag aaatgcaggg aaggacagag 6121 gagaaagtcc acatcccaaa gatcctcccc tggcacgctg ggacttattc ctgtgtggca 6181 gaaaacattc ttggtactgg acagaggggc ccgggagctg agctggatgt ccagtatcct 6241 cccaagaagg tgaccacagt gattcaaaac cccatgccga ttcgagaagg agacacagtg 6301 accctttcct gtaactacaa ttccagtaac cccagtgtta cccggtatga atggaaaccc 6361 catggcgcct gggaggagcc atcgcttggg gtgctgaaga tccaaaacgt tggctgggac 6421 aacacaacca tcgcctgcgc agcttgtaat agttggtgct cgtgggcctc ccctgtcgcc 6481 ctgaatgtcc agtatgcccc ccgagacgtg agggtccgga aaatcaagcc cctttccgag 6541 attcactctg gaaactcggt cagcctccaa tgtgacttct caagcagcca ccccaaagaa 6601 gtccagttct tctgggagaa aaatggcagg cttctgggga aagaaagcca gctgaatttt 6661 gactccatct ccccagaaga tgctgggagt tacagctgct gggtgaacaa ctccatagga 6721 cagacagcgt ccaaggcctg gacacttgaa gtgctgtatg cacccaggag gctgcgtgtg 6781 tccatgagcc cgggggacca agtgatggag gggaagagtg caaccctgac ctgtgagagc 6841 gacgccaacc ctcccgtctc ccactacacc tggtttgact ggaataacca aagcctcccc 6901 taccacagcc agaagctgag attggagccg gtgaaggtcc agcactcggg tgcctactgg 6961 tgccagggga ccaacagtgt gggcaagggc cgttcgcctc tcagcaccct caccgtctac 7021 tatagcccgg agaccatcgg caggcgagtg gctgtgggac tcgggtcctg cctcgccatc 7081 ctcatcctgg caatctgtgg gctcaagctc cagcgacgtt ggaagaggac acagagccag 7141 caggggcttc aggagaattc cagcggccag agcttctttg tgaggaataa aaaggttaga 7201 agggcccccc tctctgaagg cccccactcc ctgggatgct acaatccaat gatggaagat 7261 ggcattagct acaccaccct gcgctttccc gagatgaaca taccacgaac tggagatgca 7321 gagtcctcag agatgcagag acctcccccg gactgcgatg acacggtcac ttattcagca 7381 ttgcacaagc gccaagtggg cgactatgag aacgtcattc cagattttcc agaagatgag 7441 gggattcatt actcagagct gatccagttt ggggtcgggg agcggcctca ggcacaagaa 7501 aatgtggact atgtgatcct caaacattga gcggccgcga aggatctgcg atcgctccgg 7561 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 7621 cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 7681 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 7741 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc 7801 tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag 7861 tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag 7921 tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact 7981 cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt 8041 ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag atgaccgagt 8101 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg 8161 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg 8221 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg 8281 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag 8341 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg 8401 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt 8461 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg 8521 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg 8581 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg 8641 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gtcgacaatc 8701 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 8761 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 8821 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 8881 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 8941 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 9001 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 9061 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct 9121 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 9181 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 9241 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga 9301 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 9361 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc 9421 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 9481 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 9541 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 9601 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 9661 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 9721 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 9781 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc 9841 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 9901 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc 9961 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 10021 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 10081 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 10141 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 10201 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 10261 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 10321 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 10381 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 10441 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 10501 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 10561 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 10621 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 10681 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 10741 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 10801 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 10861 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 10921 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 10981 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 11041 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 11101 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 11161 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 11221 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 11281 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 11341 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 11401 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 11461 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 11521 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 11581 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 11641 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 11701 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 11761 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 11821 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 11881 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 11941 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 12001 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 12061 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 12121 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 12181 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 12241 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 12301 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 12361 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 12421 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 12481 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 12541 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc 12601 tg //