LOCUS V046378 9845 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V046378 VERSION V046378 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..4866 /label="DDR2(NM_006182)" /note="DDR2(NM_006182)" /gene="DDR2" CDS 4873..4899 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4903..4929 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4933..4959 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 5010..5221 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 5234..5502 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 5535..5933 /codon_start=1 /gene="Aspergillus terreus BSD" /note="confers resistance to blasticidin" /product="blasticidin S deaminase" /transl_table=1 /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG*" /label="BSD" misc_feature 5940..6528 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6602..6835 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6907..7028 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7047..7182 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7220..7236) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7244..7260 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7268..7298) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7313..7334 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7622..8210) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8381..9241) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9242..9346) /gene="bla" /label="AmpR promoter" primer_bind 9820..9836 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catgatcctg attcccagaa tgctcttggt gctgttcctg 2341 ctgctgccta tcttgagttc tgcaaaagct caggttaatc cagctatatg ccgctatcct 2401 ctgggcatgt caggaggcca gattccagat gaggacatca cagcttccag tcagtggtca 2461 gagtccacag ctgccaaata tggaaggctg gactcagaag aaggggatgg agcctggtgc 2521 cctgagattc cagtggaacc tgatgacctg aaggagtttc tgcagattga cttgcacacc 2581 ctccatttta tcactctggt ggggacccag gggcgccatg caggaggtca tggcatcgag 2641 tttgccccca tgtacaagat caattacagt cgggatggca ctcgctggat ctcttggcgg 2701 aaccgtcatg ggaaacaggt gctggatgga aatagtaacc cctatgacat tttcctaaag 2761 gacttggagc cgcccattgt agccagattt gtccggttca ttccagtcac cgaccactcc 2821 atgaatgtgt gtatgagagt ggagctttac ggctgtgtct ggctagatgg cttggtgtct 2881 tacaatgctc cagctgggca gcagtttgta ctccctggag gttccatcat ttatctgaat 2941 gattctgtct atgatggagc tgttggatac agcatgacag aagggctagg ccaattgacc 3001 gatggtgtgt ctggcctgga cgatttcacc cagacccatg aataccacgt gtggcccggc 3061 tatgactatg tgggctggcg gaacgagagt gccaccaatg gctacattga gatcatgttt 3121 gaatttgacc gcatcaggaa tttcactacc atgaaggtcc actgcaacaa catgtttgct 3181 aaaggtgtga agatctttaa ggaggtacag tgctacttcc gctctgaagc cagtgagtgg 3241 gaacctaatg ccatttcctt cccccttgtc ctggatgacg tcaaccccag tgctcggttt 3301 gtcacggtgc ctctccacca ccgaatggcc agtgccatca agtgtcaata ccattttgca 3361 gatacctgga tgatgttcag tgagatcacc ttccaatcag atgctgcaat gtacaacaac 3421 tctgaagccc tgcccacctc tcctatggca cccacaacct atgatccaat gcttaaagtt 3481 gatgacagca acactcggat cctgattggc tgcttggtgg ccatcatctt tatcctcctg 3541 gccatcattg tcatcatcct ctggaggcag ttctggcaga aaatgctgga gaaggcttct 3601 cggaggatgc tggatgatga aatgacagtc agcctttccc tgccaagtga ttctagcatg 3661 ttcaacaata accgctcctc atcacctagt gaacaagggt ccaactcgac ttacgatcgc 3721 atctttcccc ttcgccctga ctaccaggag ccatccaggc tgatacgaaa actcccagaa 3781 tttgctccag gggaggagga gtcaggctgc agcggtgttg tgaagccagt ccagcccagt 3841 ggccctgagg gggtgcccca ctatgcagag gctgacatag tgaacctcca aggagtgaca 3901 ggaggcaaca catactcagt gcctgccgtc accatggacc tgctctcagg aaaagatgtg 3961 gctgtggagg agttccccag gaaactccta actttcaaag agaagctggg agaaggacag 4021 tttggggagg ttcatctctg tgaagtggag ggaatggaaa aattcaaaga caaagatttt 4081 gccctagatg tcagtgccaa ccagcctgtc ctggtggctg tgaaaatgct ccgagcagat 4141 gccaacaaga atgccaggaa tgattttctt aaggagataa agatcatgtc tcggctcaag 4201 gacccaaaca tcatccatct attagctgtg tgtatcactg atgaccctct ctgtatgatc 4261 actgaataca tggagaatgg agatctcaat cagtttcttt cccgccacga gccccctaat 4321 tcttcctcca gcgatgtacg cactgtcagt tacaccaatc tgaagtttat ggctacccaa 4381 attgcctctg gcatgaagta cctttcctct cttaattttg ttcaccgaga tctggccaca 4441 cgaaactgtt tagtgggtaa gaactacaca atcaagatag ctgactttgg aatgagcagg 4501 aacctgtaca gtggtgacta ttaccggatc cagggccggg cagtgctccc tatccgctgg 4561 atgtcttggg agagtatctt gctgggcaag ttcactacag caagtgatgt gtgggccttt 4621 ggggttactt tgtgggagac tttcaccttt tgtcaagaac agccctattc ccagctgtca 4681 gatgaacagg ttattgagaa tactggagag ttcttccgag accaagggag gcagacttac 4741 ctccctcaac cagccatttg tcctgactct gtgtataagc tgatgctcag ctgctggaga 4801 agagatacga agaaccgtcc ctcattccaa gaaatccacc ttctgctcct tcaacaaggc 4861 gacgagctcg agtacccata cgacgtacct gattatgcaa cttatcctta tgatgtacct 4921 gattatgcta gctatcctta tgatgtccca gactacgcat agggatccgc ggccgcgaag 4981 gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga 5041 gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa 5101 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta 5161 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca 5221 gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg 5281 ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc 5341 gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc 5401 ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact 5461 ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc 5521 tacgctaggc caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag 5581 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc 5641 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt 5701 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta 5761 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg 5821 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga 5881 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagtcgaca 5941 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 6001 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 6061 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 6121 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 6181 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 6241 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 6301 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 6361 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 6421 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 6481 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta 6541 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 6601 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 6661 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 6721 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 6781 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 6841 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 6901 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 6961 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 7021 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 7081 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 7141 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 7201 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 7261 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 7321 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 7381 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 7441 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 7501 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 7561 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 7621 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 7681 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 7741 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 7801 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 7861 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 7921 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 7981 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 8041 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 8101 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 8161 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 8221 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 8281 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 8341 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 8401 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 8461 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 8521 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 8581 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 8641 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 8701 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 8761 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 8821 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 8881 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 8941 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 9001 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 9061 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 9121 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 9181 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 9241 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 9301 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 9361 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 9421 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 9481 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 9541 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 9601 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 9661 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 9721 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 9781 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 9841 agctg //